Detection Optimization of Disbond in Layered Composites with Varying Thicknesses using an Open-Ended Rectangular Waveguide

The detection of air disbond in layered dielectric composite, which is an important practical issue in many industries, is studied both theoretically and experimentally. Sensitivity of disbond detection depends on certain parameters, like the frequency of operation, the distance between the sensor and the first dielectric layer, and the layered composite geometry (conductor backed or terminated by an infinite half-space of air). The impact of all these parameters is investigated theoretically and then verified experimentally

Calibration and Measurement of Dielectric Properties of Finite Thickness Composite Sheets with Open-Ended Coaxial Sensors

The application of open-ended coaxial sensors for dielectric measurement of finite thickness composite sheets is studied. Expressions for calculation of the complex aperture admittance for two geometries are presented. These expressions are used to calculate the dielectric constant of infinite half-space as well as finite thickness slabs. A more efficient method of such calculations, using a personal computer, for low to medium loss dielectrics is demonstrated. The question of when a dielectric layer may be considered as infinitely thick is also addressed, and examples are presented. A different calibration technique (compared to the conventional ones) is described and successfully implemented. This calibration technique utilizes a dielectric sheet with known dielectric properties and thickness. Measurements for different airgaps between the open-ended coaxial line and the dielectric sheet are used to perform and enhance the calibration. The results of this calibration technique and several subsequent measurements are presented and discussed

Microwave Detection Optimization of Disbond in Layered Dielectrics with Varying Thickness

The detection sensitivity optimization of air disbond in layered dielectric composites, using an open-ended rectangular waveguide, is studied both theoretically and experimentally. The sensitivity of the disbond detection is strongly influenced by the proper choice of parameters such as the operating frequency and the layered composite geometry (conductor backed or terminated by an infinite half-space of air). The capability of optimizing the measurement system parameters to detect and estimate the thickness of a disbonded layer independent of some changes in the thickness of the dielectric coating is also demonstrated. The impact of the parameters influencing detection optimization is theoretically investigated and then experimentally verified

Microwave Noncontact Examination of Disbond and Thickness Variation in Stratified Composite Media

Numerical and experimental results of a microwave noncontact, nondestructive detection and evaluation of disbonds and thickness variations in stratified composite media are presented. The aperture admittance characteristics of a flange mounted rectangular waveguide radiating into a layered, generally lossy dielectric media backed or unbacked by a conducting sheet is modeled. The theoretical implementation is based on a Fourier transform boundary matching technique to construct the field components in each medium, coupled with a stationary form of the terminating aperture admittance of the waveguide. The model can serve as a reliable test bed for real-time examination of layered composite media. Experimental results for several cases are presented which show good agreement with the theoretical findings. This is a versatile technique for near-field in situ interrogation of stratified composite media which provides for high resolution measurements

Microwave Diagnosis of Rubber Compounds

The results of a study investigating the dielectric properties of rubber compounds and their constituents in the frequency range of 5 to 24 GHz are presented. A completely filled short circuited waveguide technique was used to conduct these dielectric measurements. The influence of carbon black content in rubber was investigated for carefully prepared rubber samples. The results showed that for all frequencies, the dielectric constant increases as a function of increasing carbon black content. The variation of dielectric constant values decreases as frequency increases. The presence of curatives in uncured rubber samples was also detected, which indicates the sensitivity of microwaves to the chemical reaction triggered by curatives

Real-Time and On-Line Near-Field Microwave Inspection of Surface Defects in Rolled Steel

The potential and limitations of near-field microwave inspection techniques for detecting various surface defects in rolled steel have been investigated. The focus of this study has been to investigate this potential for tin mill products containing gross and subtle defects including steel induced defects, roll marks, holes, scratches and gouges

Near-Field Analysis of Rectangular Waveguide Probes Used for Imaging

Near-field microwave imaging of composite structures has received considerable attention recently. The success achieved on the experimental level motivated the development of a theoretical model to describe the high quality images obtained using near-field microwave imaging [1–4]. This theoretical model will also help in building an intuitive understanding of the behavior of the fields inside dielectric materials in the near-field of an open-ended rectangular waveguide probe. A near-field microwave image is the result of several factors such as probe type (example rectangular waveguide, circular waveguide or coaxial line), field properties (i.e. main lobe, sidelobes and half power beam width, etc.), geometrical and physical properties of both the defect and the material under inspection. Thus, in order to characterize a defect, the effect of all non-defect factors needs to be taken out of an image. One of the dominant non-defect factors which influences an image significantly is the radiator field properties. Thus, it is essential to formulate the properties of the fields radiating out of an open-ended rectangular waveguide in its near-field. This knowledge will aid in formulating the forward problem when imaging a defect, and will be used to solve the inverse problem for obtaining defect properties. In this paper fields radiating out of an open-ended rectangular waveguide, into an infinite half-space of a dielectric material, are calculated and used to explain some of the features observed in experimental near-field microwave images

Photoluminescent diamond nanoparticles for cell labeling: study of the uptake mechanism in mammalian cells

Diamond nanoparticles (nanodiamonds) have been recently proposed as new labels for cellular imaging. For small nanodiamonds (size <40 nm) resonant laser scattering and Raman scattering cross-sections are too small to allow single nanoparticle observation. Nanodiamonds can however be rendered photoluminescent with a perfect photostability at room temperature. Such a remarkable property allows easier single-particle tracking over long time-scales. In this work we use photoluminescent nanodiamonds of size <50 nm for intracellular labeling and investigate the mechanism of their uptake by living cells . By blocking selectively different uptake processes we show that nanodiamonds enter cells mainly by endocytosis and converging data indicate that it is clathrin mediated. We also examine nanodiamonds intracellular localization in endocytic vesicles using immunofluorescence and transmission electron microscopy. We find a high degree of colocalization between vesicles and the biggest nanoparticles or aggregates, while the smallest particles appear free in the cytosol. Our results pave the way for the use of photoluminescent nanodiamonds in targeted intracellular labeling or biomolecule deliver

MicroRNA expression profiles in pediatric dysembryoplastic neuroepithelial tumors.

© Springer Science+Business Media New York 2015Among noncoding RNAs, microRNAs (miRNAs) have been most extensively studied, and their biology has repeatedly been proven critical for central nervous system pathological conditions. The diagnostic value of several miRNAs was appraised in pediatric dysembryoplastic neuroepithelial tumors (DNETs) using miRNA microarrays and receiving operating characteristic curves analyses. Overall, five pediatric DNETs were studied. As controls, 17 samples were used: the FirstChoice Human Brain Reference RNA and 16 samples from deceased children who underwent autopsy and were not present with any brain malignancy. The miRNA extraction was carried out using the mirVANA miRNA Isolation Kit, while the experimental approach included miRNA microarrays covering 1211 miRNAs. Quantitative real-time polymerase chain reaction was performed to validate the expression profiles of miR-1909* and miR-3138 in all samples initially screened with miRNA microarrays. Our findings indicated that miR-3138 might act as a tumor suppressor gene when down-regulated and miR-1909* as a putative oncogenic molecule when up-regulated in pediatric DNETs compared to the control cohort. Subsequently, both miRNA signatures might serve as putative diagnostic biomarkers for pediatric DNETs.Peer reviewedFinal Accepted Versio

Imaging Changes in Very Young Children with Brain Tumors Treated with Proton Therapy and Chemotherapy

ABSTRACT SUMMARY: PT promises to reduce side effects in children with brain tumors by sparing normal tissue compared with 3D conformal or intensity-modulated radiation therapy. Information is lacking about the combined effects of PT and chemotherapy in young children. We describe imaging changes in 8 very young children with localized brain tumors who received PT after chemotherapy. Mostly transient signal abnormalities and enhancement in brain parenchyma were observed by serial MR imaging, which were consistent with radiation-induced effects on normal-appearing tissue. Correlation with PT planning data revealed that the areas of imaging abnormality were located within or adjacent to the volume that received the highest radiation dose. Radiologists should be aware of these findings in children who receive PT after chemotherapy. In this report, we describe the time course of these PT-related imaging findings and correlate them with treatment and clinical outcomes. ABBREVIATIONS: AT/RT ϭ atypical teratoid rhabdoid tumor; CGE ϭ cobalt gray equivalent; CPC ϭ choroid plexus carcinoma; PNET ϭ primitive neuroectoderma