Adjustable securing base

An adjustable securing base had a surface radius of curvature which was adjusted to support an adjacent part on the base surface. The securing base comprised of a flexible thin plate with an adjustment mechanism connected on opposite edges of the base for adjusting the plate curvature. An opening in the plate was coupled to a vacuum device for securing the part adjacent to the plate surface

H-NS Regulation of IraD and IraM Antiadaptors for Control of RpoS Degradation

International audienceRpoS, the master sigma factor during stationary phase and under a variety of stress conditions, is regulated at multiple levels, including regulated degradation. Degradation is dependent upon ClpXP and the RssB adaptor protein. H-NS, a nucleoid-associated protein, affects the regulated degradation of RpoS; in the absence of H-NS, RpoS is stable. The mechanisms involved in this regulation were not known. We have found that H-NS inhibits the expression of iraD and iraM, the genes coding for two antiadaptor proteins that stabilize RpoS when overexpressed. The regulation by H-NS of iraM is independent from the previously demonstrated regulation by the PhoP/PhoQ two-component system. Moreover, differences in the behavior of several hns alleles are explained by a role for StpA, an H-NS-like protein, in the regulation of RpoS stability. This finding parallels recent observations for a role of StpA in regulation of RpoS stability in Salmonella

Demography and disorders of the French Bulldog population under primary veterinary care in the UK in 2013

Abstract Background Despite its Gallic name, the French Bulldog is a breed of both British and French origin that was first recognised by The Kennel Club in 1906. The French Bulldog has demonstrated recent rapid rises in Kennel Club registrations and is now (2017) the second most commonly registered pedigree breed in the UK. However, the breed has been reported to be predisposed to several disorders including ocular, respiratory, neurological and dermatological problems. The VetCompass™ Programme collates de-identified clinical data from primary-care veterinary practices in the UK for epidemiological research. Using VetCompass™ clinical data, this study aimed to characterise the demography and common disorders of the general population of French Bulldogs under veterinary care in the UK. Results French Bulldogs comprised 2228 (0.49%) of 445,557 study dogs under veterinary care during 2013. Annual proportional birth rates showed that the proportional ownership of French Bulldog puppies rose steeply from 0.02% of the annual birth cohort attending VetCompass™ practices in 2003 to 1.46% in 2013. The median age of the French Bulldogs overall was 1.3 years (IQR 0.6–2.5, range 0.0–13.0). The most common colours of French Bulldogs were brindle (solid or main) (32.36%) and fawn (solid or main) (29.9%). Of the 2228 French Bulldogs under veterinary care during 2013, 1612 (72.4%) had at least one disorder recorded. The most prevalent fine-level precision disorders recorded were otitis externa (14.0%, 95% CI: 12.6–15.5), diarrhoea (7.5%, 95% CI: 6.4–8.7), conjunctivitis (3.2%, 95% CI: 2.5–4.0), nails overlong (3.1%, 95% CI% 2.4–3.9) and skin fold dermatitis (3.0%, 95% CI% 2.3–3.8). The most prevalent disorder groups were cutaneous (17.9%, 95% CI: 16.3–19.6), enteropathy (16.7%, 95% CI: 15.2–18.3), aural (16.3%, 95% CI: 14.8–17.9), upper respiratory tract (12.7%, 95% CI: 11.3–14.1) and ophthalmological (10.5%, 95% CI: 9.3–11.9). Conclusions Ownership of French Bulldogs in the UK is rising steeply. This means that the disorder profiles reported in this study reflect a current young UK population and are likely to shift as this cohort ages. Otitis externa, diarrhoea and conjunctivitis were the most common disorders in French Bulldogs. Identification of health priorities based on VetCompass™ data can support evidence–based reforms to improve health and welfare within the breed

Moving from information and collaboration to action: report from the 3rd International Dog Health Workshop, Paris in April 2017

Abstract Background Breed-related health problems in dogs have received increased focus over the last decade. Responsibility for causing and/or solving these problems has been variously directed towards dog breeders and kennel clubs, the veterinary profession, welfare scientists, owners, regulators, insurance companies and the media. In reality, all these stakeholders are likely to share some responsibility and optimal progress on resolving these challenges requires all key stakeholders to work together. The International Partnership for Dogs (IPFD), together with an alternating host organization, holds biennial meetings called the International Dog Health Workshops (IDHW). The Société Centrale Canine (French Kennel Club) hosted the 3rd IDHW, in Paris, in April, 2017. These meetings bring together a wide range of stakeholders in dog health, science and welfare to improve international sharing of information and resources, to provide a forum for ongoing collaboration, and to identify specific needs and actions to improve health, well-being and welfare in dogs. Results The workshop included 140 participants from 23 countries and was structured around six important issues facing those who work to improve dog health. These included individualized breed-specific strategies for health and breeding, extreme conformations, education and communication in relation to antimicrobial resistance, behavior and welfare, genetic testing and population-based evidence. A number of exciting actions were agreed during the meeting. These included setting up working groups to create tools to help breed clubs accelerate the implementation of breed-health strategies, review aspects of extreme conformation and share useful information on behavior. The meeting also heralded the development of an online resource of relevant information describing quality measures for DNA testing. A demand for more and better data and evidence was a recurring message stressed across all themes. Conclusions The meeting confirmed the benefits from inclusion of a diverse range of stakeholders who all play relevant and collaborative parts to improve future canine health. Firm actions were set for progress towards improving breed-related welfare. The next international workshop will be in the UK in 2019 and will be organized by the UK Kennel Club

Visualizing neuroinflammation with fluorescence and luminescent lanthanide-based in situ hybridization

© 2019 The Author(s). Background: Neurokine signaling via the release of neurally active cytokines arises from glial reactivity and is mechanistically implicated in central nervous system (CNS) pathologies such as chronic pain, trauma, neurodegenerative diseases, and complex psychiatric illnesses. Despite significant advancements in the methodologies used to conjugate, incorporate, and visualize fluorescent molecules, imaging of rare yet high potency events within the CNS is restricted by the low signal to noise ratio experienced within the CNS. The brain and spinal cord have high cellular autofluorescence, making the imaging of critical neurokine signaling and permissive transcriptional cellular events unreliable and difficult in many cases. Methods: In this manuscript, we developed a method for background-free imaging of the transcriptional events that precede neurokine signaling using targeted mRNA transcripts labeled with luminescent lanthanide chelates and imaged via time-gated microscopy. To provide examples of the usefulness this method can offer to the field, the mRNA expression of toll-like receptor 4 (TLR4) was visualized with traditional fluorescent in situ hybridization (FISH) or luminescent lanthanide chelate-based in situ hybridization (LISH) in mouse BV2 microglia or J774 macrophage phenotype cells following lipopolysaccharide stimulation. TLR4 mRNA staining using LISH- and FISH-based methods was also visualized in fixed spinal cord tissues from BALB/c mice with a chronic constriction model of neuropathic pain or a surgical sham model in order to demonstrate the application of this new methodology in CNS tissue samples. Results: Significant increases in TLR4 mRNA expression and autofluorescence were visualized over time in mouse BV2 microglia or mouse J774 macrophage phenotype cells following lipopolysaccharide (LPS) stimulation. When imaged in a background-free environment with LISH-based detection and time-gated microscopy, increased TLR4 mRNA was observed in BV2 microglia cells 4 h following LPS stimulation, which returned to near baseline levels by 24 h. Background-free imaging of mouse spinal cord tissues with LISH-based detection and time-gated microscopy demonstrated a high degree of regional TLR4 mRNA expression in BALB/c mice with a chronic constriction model of neuropathic pain compared to the surgical sham model. Conclusions: Advantages offered by adopting this novel methodology for visualizing neurokine signaling with time-gated microscopy compared to traditional fluorescent microscopy are provided

Decreasing Time to Initiation of Chemotherapy for Patients Electively Admitted to a Hematologic Malignancy Service.

Background: Delays in initiating elective inpatient chemotherapy can decrease patient satisfaction and increase length of stay. At our institution, we observed that 86% of patients admitted for elective chemotherapy experienced a delay (greater than 6 hours) with a median time to chemotherapy of 18.9 hours. We developed a process improvement initiative to improve time to chemotherapy for elective chemotherapy admissions. Methods: Our outcome measure was time from admission to chemotherapy administration in patients admitted for elective chemotherapy. Process measures were identified and monitored. We collected baseline data and utilized performance improvement tools to identify key drivers. We focused on these key drivers to develop multiple plan-do-study-act (PDSA) cycles to improve our outcome measure. Once we started an intervention we collected data every two weeks to assess our intervention. Results: At the time of interim analysis, we observed a median decrease in time to chemotherapy administration from 18.9 hours to 8.85 hours (p value:0.005). Median time to lab resulted decreased from 3.17 hours to 0.00 hours. There was no change in time from signature to nurse releasing the chemotherapy. We noticed more providers were signing the chemotherapy prior to patient admission. Conclusions: By implementing new admission workflows, optimizing our use of the Electronic Medical Record to communicate among providers, and improving pre-admission planning we were able to reduce our median time to chemotherapy for elective admissions by 53.2%. Improvement still needed to meet our goals and to ensure sustainability of these ongoing efforts

Identificação dos fatores de riscos do ecossistema na maternidade por meio de análise de correspondência. I. Diarréias.

Na suinocultura intensiva as doenças são causa de prejuízo. Estas doenças tem origem multifatoriais inter-relacionadas e de etiologia complexa, podendo no entanto serem compreendidas por meios de estudos ectopatológicos.Resumo

Manutenção em campo de níveis variados de palhada de cana-de-açúcar: efeitos sobre o acúmulo de nitrogênio nas plantas.

Resumo: O objetivo do trabalho foi estudar a contribuição de diferentes níveis de palhada sobre o acumulo de nitrogênio na cana-de-açúcar. O ensaio foi instalado em Araras-SP, durante as safras de 2011-12 e 2012-13 (variedade RB-845210). Foram aplicados cinco tratamentos, com níveis de 0%, 25%, 50%, 75% e 100% (0 - 2,8- 5,7- 8,5 -11,3 t ha-1 safra 2011-12) (0 - 3 - 6,2 - 9,4 - 12,8 t ha-1 safra 2012-13) dispostos em blocos ao acaso com quatro repetições. Para acompanhar o acumulo de N nos diferentes compartimentos da planta (folhas verdes, folhas secas e colmo) de cana-de-açúcar foram utilizados os dados de massa seca (kg ha-1) e concentrações de N (g kg-1) de cada um destes componentes, obtidos em biometrias realizadas ao longo dos dois ciclos de cultivos estudados. Utilizou-se a análise de variância e regressão para o tratamento estatístico dos resultados. Foi possível concluir que: a manutenção de quantidades de palhada entre 5-12 t ha-1 promovem o mesmo acúmulo de nitrogênio no colmo em relação à ausência deste resíduo (sem palhada) somente após dois ciclos de cultivo de cana-de-açúcar; sendo que no primeiro ciclo há superioridade do tratamento sem palhada apenas sobre o maior nível deste resíduo; não há uma tendência consistente para o acúmulo de nitrogênio para as folhas verdes e secas em função de diferentes aportes de palhada sobre o solo. Abstract: The aim of this study was to evaluate the contribution of different levels of straw on the accumulation of nitrogen in sugarcane. The trial was conducted at the harvests of 2011-12 and 2012-13 in Araras-SP, using the RB-845210 variety. There were tested five levels of straw (0% - 25% - 50% - 75% - 100%), related with 2,8 - 5,7 - 8,5 -11,3 t ha -1 ( 2011-12) and 3,0 ? 6,2 ? 9,4 ? 12,8 t ha -1 (2012-13), that were arranged in a randomized block design with four replications. To determine N accumulation in sugarcane there was used dry mass (kg ha -1 ) and N concentrations (g kg -1 ) of stalk, green and dry leaves, obtained by biometry evaluations realized during the two ratoons. Variance analyses and regressions were used as statistic tool. It is possible to conclude that after two cycles of sugarcane the inputs of 5-12 t ha -1 of straw by harvest has the same contribution for nitrogen stalk accumulation than traits without straw; although in the first cycle the trait without straw is superior than the higher level of this residue. About green and dry leaves there is no tendency for the nitrogen accumulation for the input of different levels of straw in soil

On twisted Fourier analysis and convergence of Fourier series on discrete groups

We study norm convergence and summability of Fourier series in the setting of reduced twisted group $C^*$-algebras of discrete groups. For amenable groups, F{\o}lner nets give the key to Fej\'er summation. We show that Abel-Poisson summation holds for a large class of groups, including e.g. all Coxeter groups and all Gromov hyperbolic groups. As a tool in our presentation, we introduce notions of polynomial and subexponential H-growth for countable groups w.r.t. proper scale functions, usually chosen as length functions. These coincide with the classical notions of growth in the case of amenable groups.Comment: 35 pages; abridged, revised and update