Adrb2 gene polymorphisms and salbutamol responsiveness in Serbian children with asthma

Inhaled beta 2 adrenergic receptor (beta 2-AR) agonists are the mainstay of asthma therapy. The beta 2-AR protein is encoded by the ADRB2 gene and variants within this gene can have significant consequences for modulating the response to asthma therapy. This cross-sectional study performed at the University Children's Hospital in Belgrade, included 54 children with asthma. The subjects were genotyped for ADRB2 +46A gt G (Arg16Gly, rs1042713) and +79C gt G (G1n27G1u, rs1042714) polymorphisms and the association with asthma severity and response to inhaled salbutamol was examined. In Serbian asthmatic children, allele +46A was detected with a frequency of 41.7% and allele +79G was detected with a frequency of 23.1%. Allele +460 was found to be associated with a better response to inhaled salbutamol (p lt 0.05) and with mild form of asthma (p lt 0.05). Polymorphism ADRB2 +46A gt G may be a determinant of asthma severity and response to salbutamol in children with asthma. We did not find any association of +79C gt G polymorphisms with the asthma severity and bronchodilator response to inhaled salbutamol. The results of this study can be potentially useful for personalization of asthma treatment

Influence of maternal dexamethasone treatment on morphometric characteristics of pituitary GH cells and body weight in near-term rat fetuses.

Growth hormone (GH) and glucocorticoids have a powerful influence on controlling fetal growth, differentiation and maturation of numerous tissues. In the present study, the effect of maternal dexamethasone (Dx) treatment on GH cells and body weight in 19- and 21-day-old rat fetuses was investigated using immunocytochemical and morphometric methods. Pregnant female rats received daily injections of 1.0-0.5-0.5 mg Dx/kg b.w. on days 16-18 of pregnancy (experimental group), while the control group received an equal volume of saline. Dx treatment of pregnant rats enhanced immunostaining intensity and significantly increased (

Effect of phytase supplementation on growing pigs performance

Dietary concentrations of phytate are crucial for its anti-nutritive properties and its negative impact on P availability. The increase of dietary phytate level is shown to increase endogenous losses of amino acids and minerals in pigs. The partial availability of the P component of phytate to simple-stomached species attains importance as the world's rock phosphate reserves are not renewable, which could lead to a P supply crisis in the future. Supplementing phytase is becoming increasingly common as a method to improve the availability of P in plant ingredients containing high levels of phytate P. Fourty-eight pigs (Swedish Landrace boars × Dutch landrace sows) weaned at day 35 with an initial BW of 8.72±0.28 kg were used for a 40-day weaner performance study. The study was structured as a complete randomized design to evaluate the response of weaner pigs to four concentrations of microbial phytase produced by Aspergillus niger: (T1) basal diet; (T2) basal diet + 1000 FTU/kg; (T3) diet with decreased dicalcium phosphate + 1000 FTU/kg; and (T4) diet with no dicalcium phosphate + 1000 FTU/kg. Control group of piglets (T1) has achieved standard body mass while addition of phytase in meal increased body mass by 6.59% in T2 and 7.52% in T3. Phytase supplementation prevented decreased body weight gain diets where available phosphorous level was reduced by 50. The amount of consumed feed per day was not significantly different. Feed efficiency of T2 and T3 groups was by 3.23% better and of T4 for 11.29% lower compared to the control group of piglets (T1). Lower production results achieved by the use of low phosphorous diets can be avoided to a certain level by the use of microbial phytase. The use of phytase in pig diet significantly improved phosphorous availability, as well as of other mineral substances from the phytate complex. [Projekat Ministarstva nauke Republike Srbije, br. III46002

The effect of ovariectomy on thyroid c cells of adult rats

The structure and function of C cells of adult female rats after ovariectomy (Ovx) were investigated. Intact control and Ovx rats were i.p. treated with sterile olive oil for 4 weeks. Peroxidase-antiperoxidase (PAP) immunohistochemical procedure was applied to localize calcitonin (CT) in thyroid C cells while its serum content was determined by RIA method. Morphometric analyses of the C cells volume, that of their nuclei and relative volume density included stereological method with the multipurpose test system M42. Also, the average number of C cells number per mm2 was calculated. Ovx led to a significant increase in body weight (21%; p<0.005). At the same time the C cells of Ovx rats had a significantly decreased cell volume (13%; p<0.005) and their number per mm2 was increased by 59% (p<0.001) in comparison with the controls. Ovx resulted in reduction of serum CT level by 45% comparing to the corresponding controls. Based on these data it can be concluded that Ovx inhibits both the structure and function of the C cells.U ovom radu ispitivana je struktura i funkcija C ćelija adultnih ženki pacova posle ovarijektomije (Ovx). Intaktne kontrolne i Ovx životinje su tretirane i.p. sterilnim maslinovim uljem u trajanju od četiri nedelje. Za lokalizaciju kalcitonina (CT) u C ćelijama štitaste žlezde korišćena je imunohistohemijska metoda peroksidaze- antiperoksidaze (PAP). Nivo CT-a u serumu određen je RIA metodom. Morfometrijska ispitivanja volumena C ćelija, njihovih jedara i relativne volumenske gustine ćelija vršena su višenamenskim testnim sistemom M42. Takođe je izračunat broj C ćelija po mm2. Statistička obrada podataka vršena je Studentovim t-testom. Posle Ovx-a telesna masa životinja značajno je povećana za 21% (p<0.005). Volumen C ćelija Ovx pacova značajno je smanjen za 13% (p<0.005), njihov broj po mm2 povećan za 59% (p<0.001), a nivo CT-a u serumu smanjen za 45% u poređenju sa intaktnom kontrolom. Na osnovu dobijenih rezultata možemo zaključiti da Ovx deluje inhibitorno na strukturu i funkciju C ćelija štitaste žlezde kod pacova

Supplementary data for article: Mišić, D.; Šiler, B.; Gašić, U.; Avramov, S.; Živković, S.; Živković, J. N.; Milutinović, M.; Tešić, Ž. Simultaneous UHPLC/DAD/(+/-)HESI-MS/MS Analysis of Phenolic Acids and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible Application in Chemotaxonomic Studies. Phytochemical Analysis 2015, 26 (1), 72–85. https://doi.org/10.1002/pca.2538

Supporting information for: [https://doi.org/10.1002/pca.2538]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1663

Effects of intracerebroventricularly administered octreotide on gonadotrophic cells in female rats

Effects of intracerbroventricularly (ICV) administered octreotide on gonadotrophic cells (FSH and LH) of adult Wistar female rats were examined by immunocytochemical and morphometric methods. The animals received ICV three 1.0 mg doses of octreotide dissolved in 10 mL saline every second day. The controls were treated with equivalent volume of physiological saline by the same schedule. FSH- and LH-producing cells were examined using peroxidase-antiperoxidase immunocytochemical procedure. Morphometric and stereologic examinations were performed to evaluate changes in the number, volume and volume densities of gonadotrophic cells. In females treated with octreotide, the gonadotrophic cells were smaller and often pycnotic, while the number of FSH- and LH-immunopositive cells per unit area (mm2) was significantly reduced. Octreotide also induced a significant reduction of the FSH- and LH-immunoreactive cells volume, as well as of their volume densities. On the basis of these results it can be concluded that octeotride, centrally administered to adult female rats provokes changes in immunocytochemical and morphometric features of both types of gonadotrophic cells.Ispitivani su efekti oktreotida davanog intracerebroventrikularno (ICV) odraslim ženkama pacova na gonadotropne ćelije (FSH i LH). Životinje su tretirane sa ukupno tri doze od 1 mg oktreotida rastvorenog u 10 mL fiziološkog rastvora svakog drugog dana. Kontrole su na isti način primile odgovarajuću zapreminu fiziološ kog rastvora. FSH i LH ćelije su ispitivane imunocitohemijskim postupkom peroksidaza-antiperoksidaza. Da bi se ustanovile promene u broju, zapremini i zapreminskoj gustini gonadotropnih ćelija primenjen je morfometrijski kao i stereološki pristup. Kod ženki tretiranih oktreotidom, gonadotropne ćelije su bile manje i često piknotič ne. Broj imunopozitivnih FSH i LH ćelija po jedinici površine (mm2) je bio statistički značajno smanjen. Oktreotid je, takodje, izazvao značajno smanjenje zapremine FSH i LH imunoreaktivnih ćelija, kao i njihove zapreminske gustine. Na osnovu ovih rezultata, može se zaključiti da je oktreotid, dat ICV odraslim ženkama pacova doveo do promena imunocitohemijskih i morfometrijskih osobina oba ispitivana tipa gonadotropnih ćelija

Supplementary data for article: Mišić, D.; Šiler, B.; Gašić, U.; Avramov, S.; Živković, S.; Živković, J. N.; Milutinović, M.; Tešić, Ž. Simultaneous UHPLC/DAD/(+/-)HESI-MS/MS Analysis of Phenolic Acids and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible Application in Chemotaxonomic Studies. Phytochemical Analysis 2015, 26 (1), 72–85. https://doi.org/10.1002/pca.2538

Supporting information for: [https://doi.org/10.1002/pca.2538]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1663

The effect of ovariectomy on thyroid c cells of adult rats

The structure and function of C cells of adult female rats after ovariectomy (Ovx) were investigated. Intact control and Ovx rats were i.p. treated with sterile olive oil for 4 weeks. Peroxidase-antiperoxidase (PAP) immunohistochemical procedure was applied to localize calcitonin (CT) in thyroid C cells while its serum content was determined by RIA method. Morphometric analyses of the C cells volume, that of their nuclei and relative volume density included stereological method with the multipurpose test system M42. Also, the average number of C cells number per mm2 was calculated. Ovx led to a significant increase in body weight (21%; p<0.005). At the same time the C cells of Ovx rats had a significantly decreased cell volume (13%; p<0.005) and their number per mm2 was increased by 59% (p<0.001) in comparison with the controls. Ovx resulted in reduction of serum CT level by 45% comparing to the corresponding controls. Based on these data it can be concluded that Ovx inhibits both the structure and function of the C cells.U ovom radu ispitivana je struktura i funkcija C ćelija adultnih ženki pacova posle ovarijektomije (Ovx). Intaktne kontrolne i Ovx životinje su tretirane i.p. sterilnim maslinovim uljem u trajanju od četiri nedelje. Za lokalizaciju kalcitonina (CT) u C ćelijama štitaste žlezde korišćena je imunohistohemijska metoda peroksidaze- antiperoksidaze (PAP). Nivo CT-a u serumu određen je RIA metodom. Morfometrijska ispitivanja volumena C ćelija, njihovih jedara i relativne volumenske gustine ćelija vršena su višenamenskim testnim sistemom M42. Takođe je izračunat broj C ćelija po mm2. Statistička obrada podataka vršena je Studentovim t-testom. Posle Ovx-a telesna masa životinja značajno je povećana za 21% (p<0.005). Volumen C ćelija Ovx pacova značajno je smanjen za 13% (p<0.005), njihov broj po mm2 povećan za 59% (p<0.001), a nivo CT-a u serumu smanjen za 45% u poređenju sa intaktnom kontrolom. Na osnovu dobijenih rezultata možemo zaključiti da Ovx deluje inhibitorno na strukturu i funkciju C ćelija štitaste žlezde kod pacova

Effects of intracerebroventricularly administered octreotide on gonadotrophic cells in female rats

Effects of intracerbroventricularly (ICV) administered octreotide on gonadotrophic cells (FSH and LH) of adult Wistar female rats were examined by immunocytochemical and morphometric methods. The animals received ICV three 1.0 mg doses of octreotide dissolved in 10 mL saline every second day. The controls were treated with equivalent volume of physiological saline by the same schedule. FSH- and LH-producing cells were examined using peroxidase-antiperoxidase immunocytochemical procedure. Morphometric and stereologic examinations were performed to evaluate changes in the number, volume and volume densities of gonadotrophic cells. In females treated with octreotide, the gonadotrophic cells were smaller and often pycnotic, while the number of FSH- and LH-immunopositive cells per unit area (mm2) was significantly reduced. Octreotide also induced a significant reduction of the FSH- and LH-immunoreactive cells volume, as well as of their volume densities. On the basis of these results it can be concluded that octeotride, centrally administered to adult female rats provokes changes in immunocytochemical and morphometric features of both types of gonadotrophic cells.Ispitivani su efekti oktreotida davanog intracerebroventrikularno (ICV) odraslim ženkama pacova na gonadotropne ćelije (FSH i LH). Životinje su tretirane sa ukupno tri doze od 1 mg oktreotida rastvorenog u 10 mL fiziološkog rastvora svakog drugog dana. Kontrole su na isti način primile odgovarajuću zapreminu fiziološ kog rastvora. FSH i LH ćelije su ispitivane imunocitohemijskim postupkom peroksidaza-antiperoksidaza. Da bi se ustanovile promene u broju, zapremini i zapreminskoj gustini gonadotropnih ćelija primenjen je morfometrijski kao i stereološki pristup. Kod ženki tretiranih oktreotidom, gonadotropne ćelije su bile manje i često piknotič ne. Broj imunopozitivnih FSH i LH ćelija po jedinici površine (mm2) je bio statistički značajno smanjen. Oktreotid je, takodje, izazvao značajno smanjenje zapremine FSH i LH imunoreaktivnih ćelija, kao i njihove zapreminske gustine. Na osnovu ovih rezultata, može se zaključiti da je oktreotid, dat ICV odraslim ženkama pacova doveo do promena imunocitohemijskih i morfometrijskih osobina oba ispitivana tipa gonadotropnih ćelija

Active control of mechanical structures in research and education

Several crucial phases of the overall approach to development and design of smart structures are outlined in this paper. They are focused on control of lightweight mechanical structures with respect to active vibration and noise attenuation using piezoelectric actuators and sensors. The research experience and growing interest in development of smart structures have motivated introduction of courses on smart structures at universities, which are being studied extensively and with great interest by young researchers and students. Some of the author’s experiences regarding education in this field will be addressed as well