The effect of ovariectomy on thyroid c cells of adult rats

Abstract

The structure and function of C cells of adult female rats after ovariectomy (Ovx) were investigated. Intact control and Ovx rats were i.p. treated with sterile olive oil for 4 weeks. Peroxidase-antiperoxidase (PAP) immunohistochemical procedure was applied to localize calcitonin (CT) in thyroid C cells while its serum content was determined by RIA method. Morphometric analyses of the C cells volume, that of their nuclei and relative volume density included stereological method with the multipurpose test system M42. Also, the average number of C cells number per mm2 was calculated. Ovx led to a significant increase in body weight (21%; p<0.005). At the same time the C cells of Ovx rats had a significantly decreased cell volume (13%; p<0.005) and their number per mm2 was increased by 59% (p<0.001) in comparison with the controls. Ovx resulted in reduction of serum CT level by 45% comparing to the corresponding controls. Based on these data it can be concluded that Ovx inhibits both the structure and function of the C cells.U ovom radu ispitivana je struktura i funkcija C ćelija adultnih ženki pacova posle ovarijektomije (Ovx). Intaktne kontrolne i Ovx životinje su tretirane i.p. sterilnim maslinovim uljem u trajanju od četiri nedelje. Za lokalizaciju kalcitonina (CT) u C ćelijama štitaste žlezde korišćena je imunohistohemijska metoda peroksidaze- antiperoksidaze (PAP). Nivo CT-a u serumu određen je RIA metodom. Morfometrijska ispitivanja volumena C ćelija, njihovih jedara i relativne volumenske gustine ćelija vršena su višenamenskim testnim sistemom M42. Takođe je izračunat broj C ćelija po mm2. Statistička obrada podataka vršena je Studentovim t-testom. Posle Ovx-a telesna masa životinja značajno je povećana za 21% (p<0.005). Volumen C ćelija Ovx pacova značajno je smanjen za 13% (p<0.005), njihov broj po mm2 povećan za 59% (p<0.001), a nivo CT-a u serumu smanjen za 45% u poređenju sa intaktnom kontrolom. Na osnovu dobijenih rezultata možemo zaključiti da Ovx deluje inhibitorno na strukturu i funkciju C ćelija štitaste žlezde kod pacova