Cysticercosis in experimentally and naturally infected pigs: parasitological and immunological diagnosis

Our objective was to evaluate the diagnosis of swine cysticercosis by examining "ante mortem" (inspection of the tongue), "post mortem" (inspection and detailed necropsy) and ELISA for research in serum of antibodies (Ab-ELISA) and antigens (Ag-ELISA). Seven (7) pigs were experimentally infected orally with eggs of Taenia solium and another 10 were naturally infected. In the pigs experimentally infected, inspection of the tongue was negative in all animals, in the routine inspection detailed necropsy and cysticercis were identified in all of them. In pigs with heavy natural infection, inspection of the tongue identified cysticerci in two (20%), while at inspection with necropsy the parasites were identified in large quantities in all animals. In ELISA for antibody search (Ab-ELISA) TS-14 recombinant protein was used, and in search for antigen (Ag-ELISA) a monoclonal antibody against this protein. In animals experimentally infected, blood was collected weekly for 140 days. The Ab-ELISA identified an increase in titers of antibody to cysticerci 21 days after infection, and at the end of the experimental period six animals (86%) were positive to the test. The search for circulating antigens (Ag-ELISA) was positive in two pigs 28 to 91 days after infection. All naturally infected pigs were positive for Ag-ELISA and Ab-ELISA. The search for antibodies and antigens by ELISA in serum from 30 pigs of a local farm and without history of cysticercosis was negative. Thus, the use of TS-14 antigen in ELISA test (Ab-ELISA) can be useful for the diagnosis of cysticercosis in pigs with low infection

International Social Survey Programme: Family and Changing Gender Roles III - ISSP 2002

The International Social Survey Programme (ISSP) is a continuous programme of cross-national collaboration running annual surveys on topics important for the social sciences. The programme started in 1984 with four founding members - Australia, Germany, Great Britain, and the United States – and has now grown to almost 50 member countries from all over the world. As the surveys are designed for replication, they can be used for both, cross-national and cross-time comparisons. Each ISSP module focuses on a specific topic, which is repeated in regular time intervals. Please, consult the documentation for details on how the national ISSP surveys are fielded. The present study focuses on questions about family and changing gender roles.Attitude towards employment of mothers and married women; role distribution of man and woman in occupation and household; preferred extent of employment for women during different stages of child raising; attitudes towards marriage, single-parenting, cohabitation before marriage, and divorce; views on the significance of children in life; views on paid maternity leave and on financial aid for working parents; management of income in marriage or partnership; allocation of duties in the household and in family matters; time budget for housekeeping and sharing of housekeeping for both partners; frequency of disagreement about the sharing of housekeeping; decision making in matters of child raising, weekend activities and buying major things for home; principal earner (partner with higher income); stress caused by family, work and household duties (scale); estimation of general personal happiness; satisfaction with employment situation and family life; employment of mother during childhood of respondent; employment in various phases of child raising. Demography: sex; age, marital status; living together with a partner; years of school education and highest education level (degree); country specific education; current employment status; working hours per week; occupation (ISCO-88); working in private or public sector; occupational self-employment and number of employees; supervising function at work; size of household; composition of household; highest education level (degree) of spouse / partner; current employment status of spouse / partner; occupation of spouse (ISCO-88); working hours per week of spouse; spouse employed in public service; union membership; respondent`s earnings; family income; party affiliation (left-right); party affiliation (country specific) and election behaviour; religious denomination; attendance of religious services; self-placement on a top-bottom-scale. Also encoded was: region (country specific); urban-rural self-assessment; size of community (country specific); ethnic identity; mode of data collection; weighting factor.Das International Social Survey Programme (ISSP) ist ein länderübergreifendes, fortlaufendes Umfrageprogramm, das jährlich Erhebungen zu Themen durchführt, die für die Sozialwissenschaften wichtig sind. Das Programm begann 1984 mit vier Gründungsmitgliedern - Australien, Deutschland, Großbritannien und den Vereinigten Staaten - und ist inzwischen auf fast 50 Mitgliedsländer aus aller Welt angewachsen. Da die Umfragen auf Replikationen ausgelegt sind, können die Daten sowohl für länder- als auch für zeitübergreifende Vergleiche genutzt werden. Jedes ISSP-Modul konzentriert sich auf ein bestimmtes Thema, das in regelmäßigen Zeitabständen wiederholt wird. Details zur Durchführung der nationalen ISSP-Umfragen entnehmen Sie bitte der Dokumentation. Die vorliegende Studie konzentriert sich auf Fragen zu Familie und dem Wandel von Geschlechterrollen.Einstellung zur Berufstätigkeit von Müttern und verheirateten Frauen; Rollenverteilung von Mann und Frau in Beruf und Haushalt; präferierter Umfang der Berufsausübung von Frauen während verschiedener Phasen der Kindererziehung; Einstellungen zu Ehe, Alleinerziehenden, Zusammenleben vor der Ehe und Scheidung; Ansichten zur Bedeutung von Kindern für das Leben; Einstellung zu Mutterschaftsurlaub und Kindergeld; Einkommensverwaltung in der Ehe bzw. Partnerschaft; Aufgabenteilung im Haushalt und in der Familie; wöchentliches Zeitbudget für Arbeiten im Haushalt für beide Partner; Häufigkeit von Meinungsverschiedenheiten wegen der Arbeiten im Haushalt; Entscheidungsgewalt in der Kindererziehung; Entscheidungsgewalt bei Freizeitaktivitäten und Anschaffungen für den Haushalt; Hauptverdiener (Partner mit höherem Einkommen); Belastung durch Familie, Arbeit, Hausarbeit (Skala); persönliche Glückseinschätzung; Zufriedenheit mit Beruf und Familie; Berufstätigkeit der Mutter während der Kindheit des Befragten; Erwerbstätigkeit in verschiedenen Phasen der Kindererziehung. Demographie: Geschlecht; Alter; Familienstand; Zusammenleben mit einem Partner; Dauer der Schulbildung; höchster Bildungsabschluss; Bildung (länderspezifisch); Erwerbstätigkeit; wöchentliche Arbeitszeit; Beruf (ISCO-88); Beschäftigung im öffentlichen Dienst; Anzahl der Angestellten (bei Selbstständigen); Vorgesetztenfunktion; Haushaltsgröße, Zusammensetzung des Haushaltes; Erwerbstätigkeit des Partners; Beruf des Partners (ISCO-88); Wochenarbeitszeit des Partners; höchster Bildungsabschluss des Partners; Beschäftigung des Partners im öffentlichen Dienst; Gewerkschaftsmitglied; Einkommen des Befragten; Haushaltseinkommen; Parteipräferenz (links-rechts); Parteipräferenz (länderspezifisch) und Wahlverhalten bei der letzten Wahl; Religionszugehörigkeit; Kirchgangshäufigkeit; Selbsteinschätzung auf einer Oben-Unten-Skala. Zusätzlich verkodet wurde: Ortsgröße (länderspezifisch); Urbanisierungsgrad; Ortsgröße (länderspezifisch); Nationalität, ethnische Zugehörigkeit; Erhebungsmethode; Gewichtungsfaktor

Identification of putative regulatory regions and transcription factors associated with intramuscular fat content traits.

Background: Integration of high throughput DNA genotyping and RNA-sequencing data allows for the identification of genomic regions that control gene expression, known as expression quantitative trait loci (eQTL), on a whole genome scale. Intramuscular fat (IMF) content and carcass composition play important roles in metabolic and physiological processes in mammals because they influence insulin sensitivity and consequently prevalence of metabolic diseases such as obesity and type 2 diabetes. However, limited information is available on the genetic variants and mechanisms associated with IMF deposition in mammals. Thus, our hypothesis was that eQTL analyses could identify putative regulatory regions and transcription factors (TFs) associated with intramuscular fat (IMF) content traits. Results: We performed an integrative eQTL study in skeletal muscle to identify putative regulatory regions and factors associated with intramuscular fat content traits. Data obtained from skeletal muscle samples of 192 animals was used for association analysis between 461,466 SNPs and the transcription level of 11,808 genes. This yielded 1268 cis- and 10,334 trans-eQTLs, among which we identified nine hotspot regions that each affected the expression of > 119 genes. These putative regulatory regions overlapped with previously identified QTLs for IMF content. Three of the hotspots respectively harbored the transcription factors USF1, EGR4 and RUNX1T1, which are known to play important roles in lipid metabolism. From co-expression network analysis, we further identified modules significantly correlated with IMF content and associated with relevant processes such as fatty acid metabolism, carbohydrate metabolism and lipid metabolism. Conclusion: This study provides novel insights into the link between genotype and IMF content as evident from the expression level. It thereby identifies genomic regions of particular importance and associated regulatory factors. These new findings provide new knowledge about the biological processes associated with genetic variants and mechanisms associated with IMF deposition in mammals.Made available in DSpace on 2018-12-22T23:35:36Z (GMT). No. of bitstreams: 1 APIdentificationCesaretal.pdf: 9576475 bytes, checksum: 54bfa0c637b06996ccf50a3c023171bd (MD5) Previous issue date: 2018-12-20bitstream/item/189052/1/AP-Identification-Cesar-etal.pdfArticle number: 499. Na publicação: Luciana C. A. Regitano, Maurício A. Mudadu, Adhemar Zerlotini