Phytochemical and antimicrobial screening of fruits and leaves of Zizyphus lotus L. collected in North West of Algeria

The present study was conducted on medicinal plant, called Zizyphus lotus L. (Rhamnaceae) which is known as Sedra in Mascara city (North West of Algeria). This plant is very well-known in the traditional medicine to cure gastro-intestinal tract, liver and other different respiratory infections. It is communally used for its anti-inflammatory, analgesic, anti-ulcer and antidiabetic properties. In this context, this study was aimed at investigating the in vitro antimicrobial activity of ethereal extract fruits and leaves of Zizyphus lotus, collected in the locality of Bouhanifia (Mascara city). Phytochemical screening of ethereal extract revealed the presence of free quinons, entraquinons, flavonoids and tannins, alkaloids. Fruits and leaves polyphenolic amount was 563.17 and 542.93μgEGA/mgMS respectively, and 47.62 and 45.19 μgEGA/mg MS for flavonoids and 213.74 μgEQ/gdm  and 170.66 μgEQ/dm. Evaluation of the antimicrobial activity of the ethereal extract showed significant activity on the different tested bacteria.

EFFECT OF Salsola vermiculata

Algerian aride zone is rich with medicinal plant, Salsola vermiculata is one of the mosttraditional plant remedies in Bechar region. in this work, we tried to test the inhibitory effect on eight storage soft wheat fungi of various extracts of Salsola vermiculata in vitro and vivo by using grain storage by the coating method. The phytochemical screening of plants showed the detection of tannins, saponisides and flavonoids. The results show a remarkable antifungal effect by most of the extracts tested. The  decrease in the level of fungi observed in wheat samples demonstrates the effectiveness of grain storage by the coating method.Keywords: Salsola vermiculata; Antifungal activity; Coating method; Fungi; soft wheat;Storage

Microscopy of bacterial translocation during small bowel obstruction and ischemia in vivo – a new animal model

BACKGROUND: Existing animal models provide only indirect information about the pathogenesis of infections caused by indigenous gastrointestinal microflora and the kinetics of bacterial translocation. The aim of this study was to develop a novel animal model to assess bacterial translocation and intestinal barrier function in vivo. METHODS: In anaesthetized male Wistar rats, 0.5 ml of a suspension of green fluorescent protein-transfected E. coli was administered by intraluminal injection in a model of small bowel obstruction. Animals were randomly subjected to non-ischemic or ischemic bowel obstruction. Ischemia was induced by selective clamping of the terminal mesenteric vessels feeding the obstructed bowel loop. Time intervals necessary for translocation of E. coli into the submucosal stroma and the muscularis propria was assessed using intravital microscopy. RESULTS: Bacterial translocation into the submucosa and muscularis propria took a mean of 36 ± 8 min and 80 ± 10 min, respectively, in small bowel obstruction. Intestinal ischemia significantly accelerated bacterial translocation into the submucosa (11 ± 5 min, p < 0.0001) and muscularis (66 ± 7 min; p = 0.004). Green fluorescent protein-transfected E. coli were visible in frozen sections of small bowel, mesentery, liver and spleen taken two hours after E. coli administration. CONCLUSIONS: Intravital microscopy of fluorescent bacteria is a novel approach to study bacterial translocation in vivo. We have applied this technique to define minimal bacterial transit time as a functional parameter of intestinal barrier function

Phytochemical screening and antibacterial Zizyphus lotus methanolic extract against oral pathogenic bacteria

No Abstract

A New Efficient Authenticated and Key Agreement Scheme for SIP Using Digital Signature Algorithm on Elliptic Curves

Voice over Internet Protocol (VoIP) has been recently one of the more popular applications in Internet technology. It benefits lower cost of equipment, operation, and better integration with data applications than voice communications over telephone networks. However, the voice packets delivered over the Internet are not protected. The session initiation protocol (SIP) is widely used signaling protocol that controls communications on the Internet, typically using hypertext transport protocol (HTTP) digest authentication, which is vulnerable to many forms of attacks. This paper proposes a new secure authentication and key agreement scheme based on Digital Signature Algorithm (DSA) and Elliptic Curve Cryptography (ECC) named (ECDSA). Security analysis demonstrates that the proposed scheme can resist various attacks and it can be applied to authenticate the users with different SIP domains