Novel Roles for Hypoxia and Prostaglandin E2 in the Regulation of IL-8 During Endometrial Repair

The endometrium has a remarkable capacity for efficient repair; however, factors involved remain undefined. Premenstrual progesterone withdrawal leads to increased prostaglandin (PG) production and local hypoxia. Here we determined human endometrial expression of interleukin-8 (IL-8) and the roles of PGE2 and hypoxia in its regulation. Endometrial biopsy specimens (n = 51) were collected. Endometrial cells and explants were exposed to 100 nmol/L of PGE2 or 0.5% O2. The endometrial IL-8 concentration peaked during menstruation (P < 0.001) and had a significant proangiogenic effect. IL-8 was increased by PGE2 and hypoxia in secretory but not proliferative explants, which suggests that exposure to progesterone is essential. In vitro progesterone withdrawal induced significant IL-8 up-regulation in proliferative explants primed with progestins, but only in the presence of hypoxia. Epithelial cells treated simultaneously with PGE2 and hypoxia demonstrated synergistic increases in IL-8. Inhibition of HIF-1 by short hairpin RNA abolished hypoxic IL-8 induction, and inhibition of NF-κB by an adenoviral dominant negative inhibitor decreased PGE2-induced IL-8 expression (P > 0.05). Increased menstrual IL-8 is consistent with a role in repair. Progesterone withdrawal, hypoxia, and PGE2 regulate endometrial IL-8 by acting via HIF-1 and NF-κB. Hence, progesterone withdrawal may activate two distinct pathways to initiate endometrial repair

Regulation of human endometrial function: mechanisms relevant to uterine bleeding

This review focuses on the complex events that occur in the endometrium after progesterone is withdrawn (or blocked) and menstrual bleeding ensues. A detailed understanding of these local mechanisms will enhance our knowledge of disturbed endometrial/uterine function – including problems with excessively heavy menstrual bleeding, endometriosis and breakthrough bleeding with progestin only contraception. The development of novel strategies to manage these clinically significant problems depends on such new understanding as does the development of new contraceptives which avoid the endometrial side effect of breakthrough bleeding

Oral history interview with Samuel Postlethwait, 2007 Oct. 11

A retired Purdue faculty member, Department of Biology [Now Biological Sciences], he pioneered the audio tutorial system of instruction in 1961 which permitted students to "pace" themselves while learning a certain topic. He recounts his teaching method and style. "The greatest experience has been the response of students". He mentions MPATI Midwest Program in Airborne television which began in 1959 and continued until early 1960's. The recipient of many honors and awards including the Helping Students Learn Award from The Purdue Alumni Association in 1983 and the Charles F. Bessey Award From the Botanical Society of America in 1989. In retirement active in the local community including talks on wild flowers and birdlore

Oral history interview with Samuel Postlethwait, 2007 Oct. 23

A retired Purdue faculty member, Department of Biology [Now Biological Sciences], he pioneered the audio tutorial system of instruction in 1961 which permitted students to "pace" themselves while learning a certain topic. He recounts his teaching method and style. "The greatest experience has been the response of students". He mentions MPATI Midwest Program in Airborne television which began in 1959 and continued until early 1960's. The recipient of many honors and awards including the Helping Students Learn Award from The Purdue Alumni Association in 1983 and the Charles F. Bessey Award From the Botanical Society of America in 1989. In retirement active in the local community including talks on wild flowers and birdlore

Total Mercury Concentrations in Muscle Tissue from Walleye, Northern Pike, and Muskellunge Collected from Inland Lakes during Spring 2014

Prepared for the Great Lakes Indian Fish and Wildlife Commission

Total Mercury Concentrations in Muscle Tissue from Fish Captured during the Spring – Fall 2013 in Michigan Waters of Lake Superior

Prepared for the Great Lakes Indian Fish and Wildlife Commission

Total Mercury Concentrations in Sea Lamprey Transformers Collected from Rivers in Wisconsin and Michigan during Fall 2013

Prepared for the Great Lakes Indian Fish and Wildlife Commission