Performance of Some Soybean Genotypes (Glycine max L.) to Germination and Seedling Characters as Affected by Planting Dates and Phosphorus Fertilization

To investigate the performance of some sunflower genotypes to phosphorus fertilizer rates and planting dates to germination characters and seedling parameters. A laboratory experiment accompanied in seed lab during April and May 2017.The experiments included six sowing dates at 1th May, 15th May and 31th May, three soybean cultivars namely Crawford, Giza 22 and Giza 111 and three rates of phosphorus fertilizer viz. 0, 37.2 and 74.4 kg P2O5/ha.The tallest shoot, great percentages of germination, the lowermostpercentagesof dead seedand the highestcoefficient of velocitypercentage from sown on mid-May. In addition, the lowest days of germination time was produced from sown on first May. Whereas, the tallest root, the highest weight of fresh shoot and root as well as shoot dry weight from sown on end-May.The results clearly revealed that the highest percentage of germination, soot length and root length obtained from sown cv. Giza 111. The uppermostenergy of germination, shoot and root dry weight were recorded from sown Giza 22 cultivar. In addition, sown Crawford cultivar produced the highestdead seedpercentage and the lowest mean germination time.The results indicated that the lowest mean germination time and maximum percentage of coefficient of velocity, tallest shoot and root, weight of fresh shoot, shoot and dry root were obtained fromfertilizationof phosphate at the rate of31 kgP2O5/fed. It summarized that seed Giza 111 cultivar recorded the best in seed viability when sown on first May and fertilized with phosphorus fertilizer at the rate of 74.4 kg P2O5/ha

Behaviors of Some Soybean Cultivars (Glycine max L.) Yield to Planting Dates and Different Phosphorus Fertilizer Rates

Soybean production components such as planting date, cultivars and phosphorus fertilizer rates affected soybean yield. Two field experiments conducted in extensive field at El-Gahrbia district, Egypt during 2015 and 2016 seasons. The goal of this research was aimed to investigate the influence of sowing date at 1th May, 15th May and 31th May and three phosphorus fertilization rates, i.e. 0, 37.2 and 74.4 kg P2O5/ha on the performance of three soybean (Glycine max (L.) Merrl) cultivars i.e. Crawford, Giza 22 and Giza 111 on growth, yield and seed quality. The tallest plants, the thick stem, highest branches number/plant, pods number/plant, seed number/pod, weight of 1000 seed and seed yield/ha were recorded from sown early on first May in the both seasons. The tallest plants, the thick stems and highest number of branches/plant were recorded from sown Crawford cultivar. Whereas maximum number of pods/plant and number of seed/pod were found from sown Giza 111 cultivar in the both seasons. Increasing phosphorous fertilizer rates significantly increased all studied cultivars of seed yield and yield components. Tallest plant, the thick stem, the highest branches number/plant, pods number/plant, seed number/pod, weight of1000 seed and seed yield/ha were produced from phosphorous fertilizer at a rate of 74.4 kg P2O5/ha in the first and second seasons, respectively

Natural Rolling Responses of a Delta Wing in Transonic and Subsonic Flows

The unsteady, three-dimensional, full Navier-Stokes (NS) equations and the Euler equations of rigid-body dynamics are sequentially solved to simulate the natural rolling response of slender delta wings of zero thickness at moderate to high angles of attack, to transonic and subsonic flows. The governing equations of fluid flow and dynamics of the present multi-disciplinary problem are solved using the time-accurate solution of the NS equations with the implicit, upwind, Roe flux-difference splitting, finite-volume scheme and a four-stage Runge-Kutta scheme, respectively. The main focus is to analyze the effect of Mach number and angle of attack on the leading edge vortices and their breakdown, the resultant rolling motion, and overall aerodynamic response of the wing. Three cases demonstrate the natural response of a 65 deg swept, cropped delta wing in a transonic flow with breakdown of the leading edge vortices and an 80 deg swept delta wing in a subsonic flow undergoing either damped or self-excited limit-cycle rolling oscillations as a function of angle of attack. Comparisons with an experimental investigation completes this study, validating the analysis and illustrating the complex details afforded by computational investigations

Trauma as counter-revolutionary colonisation: narratives from (post)revolutionary Egypt

We argue that multiple levels of trauma were present in Egypt before, during and after the 2011 revolution. Individual, social and political trauma constitute a triangle of traumatisation which was strategically employed by the Egyptian counter-revolutionary forces – primarily the army and the leadership of the Muslim Brotherhood – to maintain their political and economic power over and above the social, economic and political interests of others. Through the destruction of physical bodies, the fragmentation and polarisation of social relations and the violent closure of the newly emerged political public sphere, these actors actively repressed the potential for creative and revolutionary transformation. To better understand this multi-layered notion of trauma, we turn to Habermas’ ‘colonisation of the lifeworld’ thesis which offers a critical lens through which to examine the wider political and economic structures and context in which trauma occurred as well as its effects on the personal, social and political realms. In doing so, we develop a novel conception of trauma that acknowledges individual, social and political dimensions. We apply this conceptual framing to empirical narratives of trauma in Egypt’s pre- and post-revolutionary phases, thus both developing a non-Western application of Habermas’ framework and revealing ethnographic accounts of the revolution by activists in Cairo

Minimally Invasive Video-Assisted Thyroidectomy and Parathyroidectomy with Intraoperative Recurrent Laryngeal Nerve Monitoring

Objective. Our goal is to study the feasibility of using intraoperative neuromonitoring (IONM) in minimally invasive video-assisted thyroidectomy and parathyroidectomy (MIVAT/P) with emphasis given to the identification of recurrent laryngeal nerve (RLN). Methods. Consecutive series of forty-seven patients with seventy-seven recurrent laryngeal nerves at risk undergoing both MIVAT/P and IONM were enrolled in this retrospective, nonrandomized analysis study. All operations were performed by the same surgeon within an academic institution setting. All patients underwent vocal cord evaluation postoperatively. Demographics and intraoperative and postoperative complications following surgery were collected. Results. Out of seventy-seven RLNs, there was one permanent unilateral RLN injury (1.29%) in a patient with advanced papillary thyroid cancer, managed by cord injection. There was another transient RLN paresis that resolved spontaneously (1.29%). There were no instances of equipment malfunction or interference. Conclusions. To our knowledge, this is the first reported MIVAT/P series from the United States of America with a standardized IONM technique. The technical feasibility of IONM seems acceptable and may serve as a meaningful adjunct to the visual identification of nerves. Neuromonitoring during MIVAT/P is effective in providing identification of laryngeal nerves and enables surgeons to feel more comfortable with MIVAT/P. Comparative series are needed for further evaluation

Playing with Fire. The Muslim Brotherhood and the Egyptian Leviathan

After the fall of Mubarak, the Muslim Brotherhood decided to act as a stabilising force, to abandon the street and to lend democratic legitimacy to the political process designed by the army. The outcome of this strategy was that the MB was first ‘burned’ politically and then harshly repressed after having exhausted its stabilising role. The main mistakes the Brothers made were, first, to turn their back on several opportunities to spearhead the revolt by leading popular forces and, second, to keep their strategy for change gradualist and conservative, seeking compromises with parts of the former regime even though the turmoil and expectations in the country required a much bolder strategy

Climbing behavior by mice as an endpoint for preclinical assessment of drug effects in the absence and presence of pain

This study evaluated climbing in mice as a tool to assess the expression and treatment of pain-related behavioral depression in male and female ICR mice. Mice were videotaped during 10-min sessions in a vertical plexiglass cylinder with wire mesh walls, and “Time Climbing” was scored by observers blind to treatments. Initial validation studies demonstrated that baseline climbing was stable across repeated days of testing and depressed by intraperitoneal injection of dilute lactic acid (IP acid) as an acute pain stimulus. Additionally, IP acid-induced depression of climbing was blocked by the positive-control non-steroidal anti-inflammatory drug (NSAID) ketoprofen but not by the negative control kappa opioid receptor agonist U69593. Subsequent studies examined effects of single-molecule opioids (fentanyl, buprenorphine, naltrexone) and of fixed-proportion fentanyl/naltrexone mixtures (10:1, 3.2:1, and 1:1) that vary in their efficacy at the mu opioid receptor (MOR). Opioids administered alone produced a dose- and efficacy-dependent decrease in climbing, and fentanyl/naltrexone-mixture data indicated that climbing in mice is especially sensitive to disruption by even low-efficacy MOR activation. Opioids administered as a pretreatment to IP acid failed to block IP acid-induced depression of climbing. Taken together, these findings support the utility of climbing in mice as an endpoint to evaluate candidate-analgesic effectiveness both to (a) produce undesirable behavioral disruption when the test drug is administered alone, and (b) produce a therapeutic blockade of pain-related behavioral depression. The failure of MOR agonists to block IP acid-induced depression of climbing likely reflects the high sensitivity of climbing to disruption by MOR agonists

Dual EZH2 and EHMT2 histone methyltransferase inhibition increases biological efficacy in breast cancer cells

Background: Many cancers show aberrant silencing of gene expression and overexpression of histone methyltransferases. The histone methyltransferases (HKMT) EZH2 and EHMT2 maintain the repressive chromatin histone methylation marks H3K27me and H3K9me, respectively, which are associated with transcriptional silencing. Although selective HKMT inhibitors reduce levels of individual repressive marks, removal of H3K27me3 by specific EZH2 inhibitors, for instance, may not be sufficient for inducing the expression of genes with multiple repressive marks. Results: We report that gene expression and inhibition of triple negative breast cancer cell growth (MDA-MB-231) are markedly increased when targeting both EZH2 and EHMT2, either by siRNA knockdown or pharmacological inhibition, rather than either enzyme independently. Indeed, expression of certain genes is only induced upon dual inhibition. We sought to identify compounds which showed evidence of dual EZH2 and EHMT2 inhibition. Using a cell-based assay, based on the substrate competitive EHMT2 inhibitor BIX01294, we have identified proof-of-concept compounds that induce re-expression of a subset of genes consistent with dual HKMT inhibition. Chromatin immunoprecipitation verified a decrease in silencing marks and an increase in permissive marks at the promoter and transcription start site of re-expressed genes, while Western analysis showed reduction in global levels of H3K27me3 and H3K9me3. The compounds inhibit growth in a panel of breast cancer and lymphoma cell lines with low to sub-micromolar IC50s. Biochemically, the compounds are substrate competitive inhibitors against both EZH2 and EHMT1/2. Conclusions: We have demonstrated that dual inhibition of EZH2 and EHMT2 is more effective at eliciting biological responses of gene transcription and cancer cell growth inhibition compared to inhibition of single HKMTs, and we report the first dual EZH2-EHMT1/2 substrate competitive inhibitors that are functional in cells

IRS2 mutations linked to invasion in pleomorphic invasive lobular carcinoma

Pleomorphic invasive lobular carcinoma (PILC) is an aggressive variant of invasive lobular breast cancer that is associated with poor clinical outcomes. Limited molecular data are available to explain the mechanistic basis for PILC behavior. To address this issue, targeted sequencing was performed to identify molecular alterations that define PILC. This sequencing analysis identified genes that distinguish PILC from classic ILC and invasive ductal carcinoma by the incidence of their genomic changes. In particular, insulin receptor substrate 2 (IRS2) is recurrently mutated in PILC, and pathway analysis reveals a role for the insulin receptor (IR)/insulin-like growth factor-1 receptor (IGF1R)/IRS2 signaling pathway in PILC. IRS2 mutations identified in PILC enhance invasion, revealing a role for this signaling adaptor in the aggressive nature of PILC