7 research outputs found
Gonadotropin-inhibitory hormone in teleosts: New insights from a basal representative, the eel
International audienceSince its discovery in birds, gonadotropin-inhibitory hormone (GnIH) has triggered investigation in the other groups of vertebrates. In the present study, we have identified a single gnih gene in the European eel (Anguilla anguilla), a representative species of a basal group of teleosts (Elopomorphs). We have also retrieved a single gnih gene in Osteoglossomorphs, as well as in more recently emerged teleosts, Clupeocephala. Phylogeny and synteny analyses allowed us to infer that one of the two gnih paralogs emerged from the teleost-specific whole genome duplication (TWGD or 3R), would have been lost shortly after the 3R, before the emergence of the basal groups of teleosts. This led to the presence of a single gnih in extant teleosts as in other vertebrates. Two gnih paralogs were still found in some teleost species, such as in salmonids, but resulting from the additional whole genome duplication that specifically occurred in this lineage (4R). Eel gnih was mostly expressed in the diencephalon part of the brain, as analyzed by quantitative real-time PCR. Cloning of eel gnih cDNA confirmed that the sequence of the GnIH precursor encoded three putative mature GnIH peptides (aaGnIH-1, aaGnIH-2 and aaGnIH-3), which were synthesized and tested for their direct effects on eel pituitary cells in vitro. Eel GnIH peptides inhibited the expression of gonadotropin subunits (lhβ, fshβ, and common a-subunit) as well as of GnRH receptor (gnrh-r2), with no effect on tshβ and gh expression. The inhibitory effect of GnIH peptides on gonadotropic function in a basal teleost is in agreement with an ancestral inhibitory role of GnIH in the neuroendocrine control of reproduction in vertebrates
Tools for designing amphipathic helical antimicrobial peptides
Methods are described for the design of amphipathic helical AMPs, to improve potency and/or increase selectivity with respect to host cells. One method is based on the statistical analysis of known helical AMPs to derive a sequence template and ranges of charge, hydrophobicity, and amphipathicity (hydrophobic moment) values that lead to broad-spectrum activity, but leaves optimization for selectivity to subsequent rounds of SAR determinations. A second method uses a small database of anuran AMPs with known potency (MIC values vs. E. coli) and selectivity (HC50 values vs. human erythrocytes), as well as the concept of longitudinal moment, to suggest sequences or sequence variations that can improve selectivity. These methods can assist in the initial design of novel AMPs with useful properties in vitro, but further development requires knowledge-based decisions and a sound prior understanding of how structural and physical attributes of this class of peptides affect their mechanism of action against bacteria and host cells
Induction of granulopoiesis of mastocytoma cells: Ultrastructural and cytochemical studies on production of serotonin in a cultured mouse mastocytoma cell line
Altered cytoskeletal structures in transformed cells exhibiting obviously metastatic capabilities
Enhanced apoptosis in transformed human lung fibroblasts after exposure to sodium butyrate
Selectivity of antimicrobial peptides: a complex interplay of multiple equilibria
Antimicrobial peptides (AMPs) attack bacterial membranes selectively, killing microbes at concentrations that cause no toxicity to the host cells. This selectivity is not due to interaction with specific receptors, but is determined by the different lipid composition of the membranes of the two cell types, and by the peculiar physico-chemical properties of AMPs, particularly their cationic and amphipathic character. However, the available data, including recent studies of peptide-cell association, indicate that this picture is excessively simplistic, because selectivity is modulated by a complex interplay of several interconnected phenomena. For instance, conformational transitions and self-assembly equilibria modulate the effective peptide hydrophobicity, the electrostatic and hydrophobic contributions to the membrane binding driving force are non-additive, and kinetic processes can play an important role in selective bacterial killing in the presence of host cells. All these phenomena, and their bearing on the final activity and toxicity of AMPs, must be considered in the definition of design principles to optimize peptide selectivity