KAHRP dynamically relocalizes to remodeled actin junctions and associates with knob spirals in Plasmodium falciparum-infected erythrocytes

The knob-associated histidine-rich protein (KAHRP) plays a pivotal role in the pathophysiology of Plasmodium falciparum malaria by forming membrane protrusions in infected erythrocytes, which anchor parasite-encoded adhesins to the membrane skeleton. The resulting sequestration of parasitized erythrocytes in the microvasculature leads to severe disease. Despite KAHRP being an important virulence factor, its physical location within the membrane skeleton is still debated, as is its function in knob formation. Here, we show by super-resolution microscopy that KAHRP initially associates with various skeletal components, including ankyrin bridges, but eventually colocalizes with remnant actin junctions. We further present a 35 Å map of the spiral scaffold underlying knobs and show that a KAHRP-targeting nanoprobe binds close to the spiral scaffold. Single-molecule localization microscopy detected ~60 KAHRP molecules/knob. We propose a dynamic model of KAHRP organization and a function of KAHRP in attaching other factors to the spiral scaffold

Spherical harmonic results for the 3D Kobayashi Benchmark suite

Spherical harmonic solutions are presented for the Kobayashi benchmark suite. The results were obtained with Ardra, a scalable, parallel neutron transport code developed at Lawrence Livermore National Laboratory (LLNL). The calculations were performed on the IBM ASCI Blue-Pacific computer at LLNL

On the performance of a survivability architecture for networked computing systems

Abstract- This research focuses on the performance and timing behavior of a two level survivability architecture. The lower level of the architecture involves attack analysis based on kernel attack signatures and survivability handlers. Higher level survivability mechanisms are implemented us-ing migratory autonomous agents. The potential for fast re-sponse to, and recovery from, malicious attacks is the main motivation to implement attack detection and survivability mechanisms at the kernel level. A timing analysis is pre-sented that suggests the real-time feasibility of the two level approach. The limits to real-time response are identified from the host and network point of view. The experimental data derived is important for risk management and analysis in the presence of malicious network and computer attacks. I

Performing three-dimensional neutral particle transport calculations on tera scale computers

A scalable, parallel code system to perform neutral particle transport calculations in three dimensions is presented. To utilize the hyper-cluster architecture of emerging tera scale computers, the parallel code successfully combines the MPI message passing and paradigms. The code's capabilities are demonstrated by a shielding calculation containing over 14 billion unknowns. This calculation was accomplished on the IBM SP ''ASCI-Blue-Pacific computer located at Lawrence Livermore National Laboratory (LLNL)