Base flipping in nucleotide excision repair

Moleculaire genetic

De praktijk van duurzame landbouw : een overzicht van initiatieven en een analyse van lessen uit de praktijk

Bij de aanvang van het project zijn twee relevante vragen geformuleerd die in deze studie zijn onderzocht: Hoe kan op dit moment de stand van zaken rond de transitie naar een duurzame landbouw worden gekarakteriseerd? Welke lessen kunnen worden geleerd van de wijze waarop de planvorming en uitvoering van systeeminnovatieve praktijkprojecten verlopen? De inventarisatie van projecten heeft een gevarieerd overzicht van in totaal 117 projecten opgeleverd. De projecten zijn gespreid over verschillende schaalniveaus, diverse thema’s en er zijn diverse actoren bij de projecten betrokke

Structural basis for H-NS-mediated trapping of RNA polymerase in the open initiation complex at the rrnB P1

Macromolecular Biochemistr

Catalytic sites for 3'- and 5' incision of Escherichia coli nucleotide excision repair are both located in UvrC.

Medical Biochemistr

Sliding wear investigation of suspension sprayed WC-Co nanocomposite coatings.

Sliding wear evaluation of nanostructured coatings deposited by Suspension High Velocity Oxy-Fuel (S-HVOF) and conventional HVOF (Jet Kote (HVOF-JK) and JP5000 (HVOF-JP)) spraying were evaluated. S-HVOF coatings were nanostructured and deposited via an aqueous based suspension of the WC-Co powder, using modified HVOF (TopGun) spraying. Microstructural evaluations of these hardmetal coatings included X-ray Diffraction (XRD) and Scanning Electron Microscopy (SEM) equipped with Energy Dispersive X-ray Spectroscopy (EDX). Sliding wear tests on coatings were conducted using a ball-on-flat test rig against steel, silicon nitride (Si3N4) ceramic and WC-6Co balls. Results indicated that nanosized particles inherited from the starting powder in S-HVOF spraying were retained in the resulting coatings. Significant changes in the chemical and phase composition were observed in the S-HVOF coatings. Despite decarburization, the hardness and sliding wear resistance of the S-HVOF coatings was comparable to the HVOF-JK and HVOF-JP coatings. The sliding wear performance was dependent on the ball-coating test couple. In general a higher ball wear rate was observed with lower coating wear rate. Comparison of the total (ball and coating) wear rate indicated that for steel and ceramic balls, HVOF-JP coatings performed the best followed by the S-HVOF and HVOF-JK coatings. For the WC-Co ball tests, average performance of S-HVOF was better than that of HVOF-JK and HVOF-JP coatings. Changes in sliding wear behavior were attributed to the support of metal matrix due to relatively higher tungsten content, and uniform distribution of nanoparticles in the S-HVOF coating microstructure. The presence of tribofilm was also observed for all test couples

Large-scale in vitro production, refolding and dimerization of PsbS in different microenvironments

Solid state NMR/Biophysical Organic Chemistr

Tuberculosis research in South Africa over the past 30 years: From bench to bedside

The South African Medical Research Council Centre for Tuberculosis Research has a rich history of high-impact research that has influenced our understating of this hyper-epidemic which is further exacerbated by the emergence and spread of drug-resistant forms of the disease. This review aims to summarise the past 30 years of research conducted in the Centre which has influenced the way that tuberculosis (TB) is diagnosed and treated. The review includes the development of new technologies for rapid screening of people with probable TB and the repurposing of human diagnostics for wildlife conservation

The effect of the DNA flanking the lesion on formation of the UvrB-DNA preincision complex - Mechanism for the UvrA-mediated loading of UvrB onto a DNA damaged site

Bio-organic Synthesi

The role of ATP binding and hydrolysis by UvrB during nucleotide excision repair

Bio-organic Synthesi