Biochemical and Genetic Characterization of the Enterococcus faecalis Oxaloacetate Decarboxylase Complex

<p>Enterococcus faecalis encodes a biotin-dependent oxaloacetate decarboxylase (OAD), which is constituted by four subunits: E. faecalis carboxyltransferase subunit OadA (termed Ef-A), membrane pump Ef-B, biotin acceptor protein Ef-D, and the novel subunit Ef-H. Our results show that in E. faecalis, subunits Ef-A, Ef-D, and Ef-H form a cytoplasmic soluble complex (termed Ef-AHD) which is also associated with the membrane. In order to characterize the role of the novel Ef-H subunit, coexpression of oad genes was performed in Escherichia coli, showing that this subunit is vital for Ef-A and Ef-D interaction. Diminished growth of the oadA and oadD single deletion mutants in citrate-supplemented medium indicated that the activity of the complex is essential for citrate utilization. Remarkably, the oadB-deficient strain was still capable of growing to wild-type levels but with a delay during the citrate-consuming phase, suggesting that the soluble Ef-AHD complex is functional in E. faecalis. These results suggest that the Ef-AHD complex is active in its soluble form, and that it is capable of interacting in a dynamic way with the membrane-bound Ef-B subunit to achieve its maximal alkalinization capacity during citrate fermentation.</p>

Feasibility Studies for Single Transverse-Spin Asymmetry Measurements at a Fixed-Target Experiment Using the LHC Proton and Lead Beams (AFTER@LHC)

International audienceThe measurement of Single Transverse-Spin Asymmetries, $A_N$ , for various quarkonium states and Drell–Yan lepton pairs can shed light on the orbital angular momentum of quarks and gluons, a fundamental ingredient of the proton-spin puzzle. The AFTER@LHC proposal combines a unique kinematic coverage and large luminosities thanks to the Large Hadron Collider beams to deliver precise measurements, complementary to the knowledge provided by collider experiments such as at RHIC. In this paper, we report on sensitivity studies for $J/\psi$ , $\varUpsilon$ and Drell–Yan $A_N$ done using the performance of LHCb-like or ALICE-like detectors, combined with polarised gaseous hydrogen and helium-3 targets. In particular, such analyses will provide us with new insights and knowledge about transverse-momentum-dependent parton distribution functions for quarks and gluons and on twist-3 collinear matrix elements in the proton and the neutron