Metabolite profiling characterises chemotypes of Musa diploids and triploids at juvenile and preflowering growth stages

Open Access Journal; Published online: 15 March 2019Bananas (Musa spp.) are consumed worldwide as dessert and cooking types. Edible banana varieties are for the most part seedless and sterile and therefore vegetatively propagated. This confers difficulties for breeding approaches against pressing biotic and abiotic threats and for the nutritional enhancement of banana pulp. A panel of banana accessions, representative of the diversity of wild and cultivated bananas, was analysed to assess the range of chemotypes available globally. The focus of this assessment was banana leaves at two growth stages (juvenile and pre-flowering), to see when during the plant growth metabolic differences can be established. The metabolic data corresponded to genomic trends reported in previous studies and demonstrated a link between metabolites/pathways and the genomes of M. acuminata and M. balbisiana. Furthermore, the vigour and resistance traits of M. balbisiana was connected to the phenolic composition and showed differences with the number of B genes in the hybrid accessions. Differences in the juvenile and pre-flowering data led to low correlation between the growth stages for prediction purposes

Molecular cloning and transcriptional regulation of ompT , a ToxR-repressed gene in Vibrio cholerae

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72189/1/j.1365-2958.2000.01699.x.pd

A metabolomics approach to the assessment of banana diversity and traits

Banana (Musa) is one of the most important economic and staple crops in the world. The majority of edible cultivated banana species arises from two species of the Eumusa group, Musa acuminata (A genome) and Musa balbisiana(B genome). In order to assess the biochemical diversity that exists in our banana germplasm collections multi-platform metabolomics platforms has been established for banana. These include LC-MS in untargeted and targeted mode, GC-MS based metabolite profiling and targeted UPLC-PDA for compounds such as carotenoids where MS ionisation is poor. Metabolomic finger printing and complementary targeted analysis has been performed on in vitro vegetative material for 20 diverse Musa accessions, including diploid varieties, wild Musa acuminata and Musa balbisiana as well as different triploids and distant wild species (Musa ornata). The data allowed the separation of the genotypes on the basis of genotypes and differentiating metabolites identified between accessions. Comparisons with field grown material was carried out in selected cases and clear correlation was observed including the potential to predict fruit phenotypes on vegetative profiles. These robust techniques can now be utilised in combination with of omic approaches to characterise consumer and agronomic traits within breeding populations