Evaluating Primary Blast Effects In Vitro

Exposure to blast events can cause severe trauma to vital organs such as the lungs, ears, and brain. Understanding the mechanisms behind such blast-induced injuries is of great importance considering the recent trend towards the use of explosives in modern warfare and terrorist related incidents. To fully understand blast-induced injury, we must first be able to replicate such blast events in a controlled environment using a reproducible method. In this technique using shock tube equipment, shock waves at a range of pressures can be propagated over live cells grown in 2D, and markers of cell viability can be immediately analyzed using a redox indicator assay and the fluorescent imaging of live and dead cells. This method demonstrated that increasing the peak blast overpressure to 127 kPa can stimulate a significant drop in cell viability when compared to untreated controls. Test samples are not limited to adherent cells, but can include cell suspensions, whole-body and tissue samples, through minor modifications to the shock tube setup. Replicating the exact conditions that tissues and cells experience when exposed to a genuine blast event is difficult. Techniques such as the one presented in this article can help to define damage thresholds and identify the transcriptional and epigenetic changes within cells that arise from shock wave exposure

Gapped tunneling spectra in the normal state of Pr2−x_{2-x}Cex_xCuO4_4

We present tunneling data in the normal state of the electron doped cuprate superconductor Pr$_{2-x}$Ce$_x$CuO$_4$ for three different values of the doping $x$. The normal state is obtained by applying a magnetic field greater than the upper critical field, $H_{c2}$ for $T < T_c$. We observe an anomalous normal state gap near the Fermi level. From our analysis of the tunneling data we conclude that this is a feature of the normal state density of states. We discuss possible reasons for the formation of this gap and its implications for the nature of the charge carriers in the normal and the superconducting states of cuprate superconductors.Comment: 7 pages ReVTeX, 11 figures files included, submitted to PR

Structure factor and thermodynamics of rigid dendrimers in solution

The ''polymer reference interaction site model'' (PRISM) integral equation theory is used to determine the structure factor of rigid dendrimers in solution. The theory is quite successful in reproducing experimental structure factors for various dendrimer concentrations. In addition, the structure factor at vanishing scattering vector is calculated via the compressibility equation using scaled particle theory and fundamental measure theory. The results as predicted by both theories are systematically smaller than the experimental and PRISM data for platelike dendrimers.Comment: 7 pages, 5 figures, submitte

Demethylation of ITGAV accelerates osteogenic differentiation in a blast-induced heterotopic ossification in vitro cell culture model

Trauma-induced heterotopic ossification is an intriguing phenomenon involving the inappropriate ossification of soft tissues within the body such as the muscle and ligaments. This inappropriate formation of bone is highly prevalent in those affected by blast injuries. Here, we developed a simplified cell culture model to evaluate the molecular events involved in heterotopic ossification onset that arise from the shock wave component of the disease. We exposed three subtypes of human mesenchymal cells in vitro to a single, high-energy shock wave and observed increased transcription in the osteogenic master regulators, Runx2 and Dlx5, and significantly accelerated cell mineralisation. Reduced representation bisulfite sequencing revealed that the shock wave altered methylation of gene promoters, leading to opposing changes in gene expression. Using a drug to target ITGAV, whose expression was perturbed by the shock wave, we found that we could abrogate the deposition of mineral in our model. These findings show how new therapeutics for the treatment of heterotopic ossification can be identified using cell culture models

Stick-slip instability for viscous fingering in a gel

The growth dynamics of an air finger injected in a visco-elastic gel (a PVA/borax aqueous solution) is studied in a linear Hele-Shaw cell. Besides the standard Saffmann-Taylor instability, we observe - with increasing finger velocities - the existence of two new regimes: (a) a stick-slip regime for which the finger tip velocity oscillates between 2 different values, producing local pinching of the finger at regular intervals, (b) a ``tadpole'' regime where a fracture-type propagation is observed. A scaling argument is proposed to interpret the dependence of the stick-slip frequency with the measured rheological properties of the gel.Comment: 7 pages, 4 figures. Submitted to Europhysics Letter

Dynamical Jahn-Teller Effect and Berry Phase in Positively Charged Fullerene I. Basic Considerations

We study the Jahn-Teller effect of positive fullerene ions $^2$C$_{60}^{+}$ and $^1$C$_{60}^{2+}$. The aim is to discover if this case, in analogy with the negative ion, possesses a Berry phase or not, and what are the consequences on dynamical Jahn-Teller quantization. Working in the linear and spherical approximation, we find no Berry phase in $^1$C$_{60}^{2+}$, and presence/absence of Berry phase for coupling of one $L=2$ hole to an $L=4$/$L=2$ vibration. We study in particular the special equal-coupling case ($g_2=g_4$), which is reduced to the motion of a particle on a 5-dimensional sphere. In the icosahedral molecule, the final outcome assesses the presence/absence of a Berry phase of $\pi$ for the $h_u$ hole coupled to $G_g$/$H_h$ vibrations. Some qualitative consequences on ground-state symmetry, low-lying excitations, and electron emission from C$_{60}$ are spelled out.Comment: 31 pages (RevTeX), 3 Postscript figures (uuencoded

Processing of strong flux trapping high T(subc) oxide superconductors: Center director's discretionary fund

Magnetic suspension effect was first observed in samples of YBa2Cu3O7/AgO(Y-123/AgO) composites. Magnetization measurements of these samples show a much larger hysteresis which corresponds to a large critical current density. In addition to the Y-123AgO composites, recently similar suspension effects in other RE-123/AgO, where RE stands for rare-Earth elements, were also observed. Some samples exhibit even stronger flux pinning than that of the Y-123/AgO sample. An interesting observation was that in order to form the composite which exhibits strong flux trapping effect the sintering temperature depends on the particular RE-123 compound used. The paper presents the detailed processing conditions for the formation of these RE-123/AgO composites, as well as the magnetization and critical field data

Cancer mortality and morbidity in employees of the United Kingdom Atomic Energy Authority, 1946-86.

In further analyses of a cohort of 39,718 United Kingdom Atomic Energy Authority employees after 7 more years follow-up, cancer mortality, based on 1,506 deaths in 1946-86, was 20% below the national average. Prostatic cancer mortality showed a statistically significant association with external radiation exposure, largely confined to men who were also monitored for internal contamination by radionuclides other than plutonium. Prostatic cancer mortality was highest in radiation workers at Winfrith. In women monitored for radiation exposure, mortality from cancer of the uterus (including the cervix uteri) was increased relative to other employees, and, showed a statistically significant association with external radiation exposure. While there were some other statistically significant results, as would be expected by chance alone when multiple comparisons are made, there were no other cancer sites with consistently exceptional findings. Point estimates for risk associated with increasing exposure to radiation suggest a decrease of four deaths per 10(4) person-years per Sv for leukaemia and an increase of 20 deaths for all cancers except leukaemia, but confidence intervals indicate that a wide range of values are compatible with the data. Cancer morbidity based on 1,699 registrations in 1971-84 was 12% below the national average. Findings from site-specific analyses largely replicated those of the mortality analyses

Research on the Geography of Agricultural Change: Redundant or Revitalized?

Future research directions for agricultural geography were the subject of debate in Area in the late 1980s. The subsequent application of political economy ideas undoubtedly revived interest in agricultural research. This paper argues that agricultural geography contains greater diversity than the dominant political economy discourse would suggest. It reviews ‘other’ areas of agricultural research on policy, post-productivism, people, culture and animals, presenting future suggestions for research. They should ensure that agricultural research continues revitalized rather than redundant into the next millennium

The hydrogen clock to infer the upper stellar mass

The most massive stars dominate the chemical enrichment, mechanical and radiative feedback, and energy budget of their host environments. Yet how massive stars initially form and how they evolve throughout their lives is ambiguous. The mass loss of the most massive stars remains a key unknown in stellar physics, with consequences for stellar feedback and populations. In this work, we compare grids of very massive star (VMS) models with masses ranging from 80-1000Msun, for a range of input physics. We include enhanced winds close to the Eddington limit as a comparison to standard O-star winds, with consequences for present-day observations of ~50-100Msun stars. We probe the relevant surface H abundances (Xs) to determine the key traits of VMS evolution compared to O stars. We find fundamental differences in the behaviour of our models with the enhanced-wind prescription, with a convergence on the stellar mass at 1.6 Myr, regardless of the initial mass. It turns out that Xs is an important tool in deciphering the initial mass due to the chemically homogeneous nature of VMS above a mass threshold. We use Xs to break the degeneracy of the initial masses of both components of a detached binary, and a sample of WNh stars in the Tarantula nebula. We find that for some objects, the initial masses are unrestricted and, as such, even initial masses of the order 1000Msun are not excluded. Coupled with the mass turnover at 1.6 Myr, Xs can be used as a 'clock' to determine the upper stellar mass.Comment: Accepted for publication in MNRAS, 14 figure