Chromosome 9p deletion in clear cell renal cell carcinoma predicts recurrence and survival following surgery

BACKGROUND: Wider clinical applications of 9p status in clear cell renal cell carcinoma (ccRCC) are limited owing to the lack of validation and consensus for interphase fluorescent in situ hybridisation (I-FISH) scoring technique. The aim of this study was to analytically validate the applicability of I-FISH in assessing 9p deletion in ccRCC and to clinically assess its long-term prognostic impact following surgical excision of ccRCC. METHODS: Tissue microarrays were constructed from 108 renal cell carcinoma (RCC) tumour paraffin blocks. Interphase fluorescent in situ hybridisation analysis was undertaken based on preset criteria by two independent observers to assess interobserver variability. 9p status in ccRCC tumours was determined and correlated to clinicopathological variables, recurrence-free survival and disease-specific survival. RESULTS: There were 80 ccRCCs with valid 9p scoring and a median follow-up of 95 months. Kappa statistic for interobserver variability was 0.71 (good agreement). 9p deletion was detected in 44% of ccRCCs. 9p loss was associated with higher stage, larger tumours, necrosis, microvascular and renal vein invasion, and higher SSIGN (stage, size, grade and necrosis) score. Patients with 9p-deleted ccRCC were at a higher risk of recurrence (P=0.008) and RCC-specific mortality (P=0.001). On multivariate analysis, 9p deletion was an independent predictor of recurrence (hazard ratio 4.323; P=0.021) and RCC-specific mortality (hazard ratio 4.603; P=0.007). The predictive accuracy of SSIGN score improved from 87.7% to 93.1% by integrating 9p status to the model (P=0.001). CONCLUSIONS: Loss of 9p is associated with aggressive ccRCC and worse prognosis in patients following surgery. Our findings independently confirm the findings of previous reports relying on I-FISH to detect 9p (CDKN2A) deletion

The importance of Antarctic krill in biogeochemical cycles

Antarctic krill (Euphausia superba) are swarming, oceanic crustaceans, up to two inches long, and best known as prey for whales and penguins – but they have another important role. With their large size, high biomass and daily vertical migrations they transport and transform essential nutrients, stimulate primary productivity and influence the carbon sink. Antarctic krill are also fished by the Southern Ocean’s largest fishery. Yet how krill fishing impacts nutrient fertilisation and the carbon sink in the Southern Ocean is poorly understood. Our synthesis shows fishery management should consider the influential biogeochemical role of both adult and larval Antarctic krill

Comparative genomic analysis of innate immunity reveals novel and conserved components in crustacean food crop species

Abstract Background Growing global demands for crustacean food crop species have driven large investments in aquaculture research worldwide. However, large-scale production is susceptible to pathogen-mediated destruction particularly in developing economies. Thus, a thorough understanding of the immune system components of food crop species is imperative for research to combat pathogens. Results Through a comparative genomics approach utilising extant data from 55 species, we describe the innate immune system of the class Malacostraca, which includes all food crop species. We identify 7407 malacostracan genes from 39 gene families implicated in different aspects of host defence and demonstrate dynamic evolution of innate immunity components within this group. Malacostracans have achieved flexibility in recognising infectious agents through divergent evolution and expansion of pathogen recognition receptors genes. Antiviral RNAi, Toll and JAK-STAT signal transduction pathways have remained conserved within Malacostraca, although the Imd pathway appears to lack several key components. Immune effectors such as the antimicrobial peptides (AMPs) have unique evolutionary profiles, with many malacostracan AMPs not found in other arthropods. Lastly, we describe four putative novel immune gene families, potentially representing important evolutionary novelties of the malacostracan immune system. Conclusion Our analyses across the broader Malacostraca have allowed us to not only draw analogies with other arthropods but also to identify evolutionary novelties in immune modulation components and form strong hypotheses as to when key pathways have evolved or diverged. This will serve as a key resource for future immunology research in crustacean food crops

Information Systems Development Plan for Sta. Teresa Funeral Homes

Nowadays, it's hard to imagine a business without IT infrastructure. Some years ago, most companies wouldn't even have it because they deem it not business-friendly. Fast forward to today, any company that fails to incorporate IT into their business process is unnoticed by their competition.Consequently, our team has decided to apply two systems that can help to improve the business process and to halt traditional routine of the company. Production Monitoring System (PMS) is responsible for controlling and stabilizing the production line to stop over-production and to check business performance and operation.Moreover, in terms of human power, the primary step once an employee entered to the firm is attendance. It would be a waste of time if manual system will still be applied and can greatly affect one's performance. Biometric Time and Attendance System (BTAS) should also be implemented. It gives a high level of accuracy and efficiently maintains employee attendance records.Thus, imposing these systems and other computer-related solution can be a great way to eradicate errors and to increase business productivity and performance

Social Structural Factors on Academic Outcomes and Satisfaction in Online Distance Education Courses Among College Students in Panabo City

This study assessed and determined the significant relationship between two variables: Social Structural Factors on Academic Outcomes and Satisfaction in Online Distance Education Courses. The study participants are college students, and researchers used quota sampling since the respondents are within Panabo City. This study used a  correlational research design with proper statistical tools such as Anova, Shapiro-Wilk Test, and Wilcoxon Signed-Rank Test. The adapted research questions break down into the following parts: Demographic Profile of the Study, Social Structural Factors on Academic Outcomes, and Satisfaction in Online Distance Education Courses, which data hoarded through online Google Forms. In the result, the p-value is 2.2e-16 is less than the 0.05 significance level. Therefore, there is a significant difference between the Level of Social Structural Factors on Academic Outcomes and the Level of Satisfaction in Online Distance Education Courses. Hence, it shows that Social Structural on Academic Performance influences Satisfaction in Online Distance Education Courses

Sustaining aquaculture by developing human capacity and enhancing opportunities for women

People are at the heart of sustaining aquaculture. Development of human capacity and gender, therefore, is an important human dimension. Human capacity development (HCD) was a major thrust of the 2000 Bangkok Declaration and Strategy, but gender was not addressed. The Food and Agriculture Organization of the United Nation's (FAO) Strategic Framework for Human Capacity Development (HCD) emphasized building human capacity in a coherent fashion at four levels - in individuals, organizations, sectors/networks and in the overall enabling environment. Although strategic HCD in aquaculture has not received attention, substantial HCD has occurred in aquaculture education and training. Aquaculture departments in universities, aquaculture research institutes, networks and professional societies all include training as central activities. Women are active participants in aquaculture supply chains, but a dearth of gender-disaggregated information hampers accurate understanding of their contribution. Research results and FAO National Aquaculture Sector Overview (NASO) fact sheets show that female participation rates vary by type and scale of enterprise and country. Women are frequently active in hatcheries and dominate fish processing plant labourers. Women's work in small-scale aquaculture frequently is unrecognized, under or unpaid. Most aquaculture development projects are not gender sensitive, and aquaculture success stories often do not report gender dimensions; projects can fail if their designs do not include gender. Lacking gender-disaggregated data on participation rates and trends in education, we conducted a preliminary survey of aquaculture tertiary institutes in Africa, Asia, Europe and North America. The percentage of female graduates in aquaculture increased considerably over the last four decades, from zero or low numbers in the 1970s to recent rates of around 30-60 percent; rates vary both by country and within countries. No data are available to track whether female graduates are entering successful careers in aquaculture. To accelerate HCD to meet the needs of aquaculture growth, commodity and theme priorities for HCD must be established. Educational institutions should cooperate and harmonize work programmes and overcome language barriers. Aquaculture education needs the best students and should help prepare them for rewarding careers. More social science content is needed in aquaculture curricula to groom graduates for management and leadership roles. The gender balance in aquaculture faculty could be improved by recruiting and retaining more women. Gender should be put firmly on the policy agenda and built into normative instruments, old and new, complemented by the collection of gender-disaggregated data for aquaculture supply chains. Women should be empowered through gender equity in access to financial, natural, training and market resources. Women in aquaculture should not be stereotyped as 'small-scale' and poor. Women are often hampered by systemic barriers such as lack of legal rights. Women should be encouraged to build their management, leadership and entrepreneural skills. In circumstances where rural men have migrated for work, small-scale aquaculture has proven a suitable livelihood option to reduce the pressure on women. Because postharvest processing and fish trade are feminized occupations, gender equity deserves special attention in fair trade and fish certification schemes. HCD and gender are receiving more attention in rehabilitation efforts to assist survivors from disease and natural disasters