17 research outputs found
Epidemiology of Human Parvovirus 4 Infection in Sub-Saharan Africa
Human parvovirus 4 infections are primarily associated with parenteral exposure in western countries. By ELISA, we demonstrate frequent seropositivity for antibody to parvovirus 4 viral protein 2 among adult populations throughout sub-Saharan Africa (Burkina Faso, 37%; Cameroon, 25%; Democratic Republic of the Congo, 35%; South Africa, 20%), which implies existence of alternative transmission routes
Seroprevalence of fecal-oral transmitted hepatitis A and E virus antibodies in Burkina Faso.
Hepatitis A virus (HAV) and hepatitis E virus (HEV) infections occur chiefly as a result of unhygienic conditions. The purpose of this study was to assess the seroprevalence of antibodies to both viruses in central Burkina Faso in the absence of a recorded hepatitis epidemic. Serum samples from 178 blood donors (131 males and 47 females) and from 189 pregnant women were collected from November 2010 to March 2012, at blood banks and medical centers in Burkina Faso. An immunochromatography test was used to screen for Anti-HAV IgM and IgG in a subgroup of 91 blood donors and 100 pregnant women. The seroprevalence of anti-HAV IgG was 14.3% [CI95, 7.1-21.4%] for all blood donors and 23% [CI95, 14.8-31.2%] for pregnant women. Anti-HEV IgG were detected using the ELISA kits Dia.pro and Wantai and were found in 19.1% [CI95, 13.3-24.9%] of the blood donors and 11.6% [CI95, 7.1-16.2%] of the pregnant women. The seroprevalences of anti-HAV and anti-HEV IgGs did not differ significantly between men and women blood donors. Anti-HAV IgM was detected in 3.3% of the blood donors and in 2% of the pregnant women. These findings for asymptomatic individuals indicate that the HAV and HEV circulate at low but significant levels. This is the first evaluation of the acute hepatitis virus burden in Burkina Faso and the underlying epidemiologic status of the population
Prevalence of anti-HAV IgG and IgM in pregnant women and blood donors.
<p>Prevalence of anti-HAV IgG and IgM in pregnant women and blood donors.</p
Evaluation of relation-ship between Wantai and Dia.Pro test results in positive samples.
<p>Crosses indicate blood donor samples and red squares samples from pregnant women. Linear correlations are significant within the two group but with significantly less dispersion of the blood donor samples than of the samples from pregnant women. The blue line indicates the cut-off level for the Wantai kit, and the red line the cut-off for the Dia.Pro kit.</p
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Transfusion safety on the African continent: an international quality control of virus testing in blood banks.
BackgroundFollowing World Health Organization recommendations that a quality control (QC) system be implemented in African blood centers, a pilot study of the performance of human immunodeficiency virus antibody (anti-HIV), hepatitis B surface antigen (HBsAg), and hepatitis C virus antibody (anti-HCV) testing by several Sub-Saharan African blood centers was initiated.Study design and methodsA reference laboratory sent a panel of 25 samples to six African blood center laboratories. The panel included eight negative samples; four anti-HIV-1–, one anti-HIV-2–, four anti-HCV–, and five HBsAg-positive samples; and three samples consisting of mixtures of two sera to mimic coinfections. Sensitivity, specificity, and overall quality (correct positive or negative status) scores were calculated.ResultsFrom the 21 sets of results obtained (seven for each virus), eight were from rapid tests (two for HIV, three for HBV, and three for HCV) and 13 were from enzyme immunoassays (EIAs; all HIV EIAs were antigen/antibody combination assays). Overall assay sensitivity was 98% for HIV, 75% for HBV, and 88% for HCV; agreement between blood centers using the same assay was good. Sensitivity of rapid tests was notably poorer than EIAs, with overall sensitivity quality scores of 64.5% for rapid tests (20% for HBsAg rapid tests) compared to 100% for EIAs. The overall specificity quality scores were 98.3 and 94.5% for EIAs and rapid tests, respectively.ConclusionsThis pilot QC study organized for blood centers of Sub-Saharan Africa showed the feasibility of the approach despite some logistic constraints. Although interlaboratory variability was small, the poor performance of rapid tests, especially for HBsAg, raises policy questions about their use as the only screening assay
Prevalence of anti-HEV IgG in pregnant women and blood donors per sampling period (Dia.Pro test).
<p>Prevalence of anti-HEV IgG in pregnant women and blood donors per sampling period (Dia.Pro test).</p
Distribution of anti-HAV IgG and IgM in blood donors according to age group.
<p>Distribution of anti-HAV IgG and IgM in blood donors according to age group.</p
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Characteristics of blood donors and donated blood in sub-Saharan Francophone Africa.
BackgroundThe importance of blood safety in public health was recognized long ago, and data are essential to plan strategies to improve the status. This study aims to obtain data on blood donor and blood donation characteristics that would complement blood safety data from national and international organizations.Materials and methodsA questionnaire was sent to seven Francophone countries (Burkina Faso, Cameroon, Congo, Ivory Coast, Mali, Niger, and Rwanda) and was structured to obtain objective data on blood donors and donated blood and in administrative and technical organization.ResultsThe results reflect a poor level of organization of blood transfusion centers in large regions of the African continent, insufficient supply of blood products, high prevalence of transfusion-transmitted infections, limited financial resources, a lack of well-trained personnel, and cultural obstacles. Six countries had less than 50% of their personnel trained in transfusion medicine. Only one country had the entire standard operating procedure written. Female donors represented less than 30% of the donors and the range of percentage of hepatitis B found in donors was 2.76% to 18.96%.ConclusionThe inclusion of these regions in future blood safety surveys and in the development of national blood transfusion programs is essential and will undoubtedly require the assistance of international organizations