Introduction to an agenda in the laboratory course for applied food science : Preparation and development of sports drinks

At Musashigaoka College, the "Preparation and Development of Sports Drinks" class is conducted in the laboratory course for applied food science. This paper introduces our approach in this course, and reports student awareness regarding sports drinks, together with the educational effectiveness as well as student\u27s evaluation of the laboratory course

A nutritional evaluation of some soft drinks as supply sources for vitamin C

A novel index (designated PAIM), in which nutritional losses as a result of cooking and practical intake in a typical meal were considered, was applied to the evaluation of ascorbic acid content in several commercially available soft drinks. This index successfully enabled a comparative examination between various foods and drinks as supply sources of ascorbic acids. Employing this index, in the seven soft drinks tested, two products were assessed as effective sources for ascorbic acid affording values nearly satisfying the daily recommended dietary allowance; furthermore, the remaining five products were superior to some foods after cooking. These results suggested that soft drinks might be regarded as an off-handed optional source of ascorbic acid

Screening for biological activity of coffee extracts

An investigation regarding the biological activity of coffee extracts was conducted based on their antibacterial, antifungal, antiviral, TNF-a production promoting, neurite outgrowth promoting, melanin synthesis inhibiting, telomerase inhibiting and human hair outer root sheath cell outgrowth promoting actions. As a result, methanol (MeOH) and hot water extracts of Toraja coffee (sour taste) and Mandarin coffee (bitter taste) demonstrated anti-IHNV, anti- human influenza virus and melanin synthesis inhibiting activities. In particular, melanin synthesis inhibiting activity of MeOH and hot water extracts was comparable to that of arbutin, an active substance widely used to whitening cosmetics. This finding is promising with respect to the development of new natural whitening cosmetics supplemented with coffee extracts

Anti-Infectious Hematopoietic Necrosis Virus (IHNV) Substances Produced by Bacteria from Aquatic Environment

Attempts were made to classify the antiviral properties of bacteria which has been isolated from various environmental waters and had the anti-infectious effects on infectious hematopoietic necrosis virus (IHNV). The majority of these bacterial strains were considered to produce the substances that cause direct inactivation of virus or inhibition of viral invasion into CHSE-214 cells. While a few of the isolates were considered to produce the substances that cause inhibition of viral replication. Among the four strains selected, two strains of Pseudomonas produced heat-stable and relatively low molecular weight substances; one strain of Alteromonas produced a high molecular weight, heat-labile substance; and one strain of Pseudomonas produced at least two kinds of substances unstable against heating

Purification of an Antiviral Substance Produced by Alteromonas sp. and Its Virucidal Activity against Fish Viruses

An antiviral substance of a high molecular weight, low cytotoxity and potent virucidal activity was purified from the culture supernatant of a marine Alteromonas sp. 48HS-27. Maximum production of this antiviral substance by the strain in MCYG broth was attained by 72 h-incubation at 25℃. By the purification procedure involving ultrafiltration, precipitation with ammonium sulfate and acetone, gel filtration and native-polyacrylamide gel electrophoresis (PAGE), a polypeptide (48HS-27A) with antiviral activity was obtained at a 270-fold purification with 6.20% yield from the culture supernatant. Molecular weight of the purified 48HS-27A was estimated as approximately 52kDa by both native and sodium dodecyl sulfate (SDS) PAGE. The 50% infection inhibitory concentrations of this substance were from 0.09 to 2.51 μg/ml against one herpesvirus and five rhabdoviruses, whereas the minimal cytotoxic concentration of the substance was 144μg/ml against FHM and CHSE-214 cells. The purified 48HS-27A had proteolytic activity against casein and bovine serum albumin