Antifungal susceptibilities, varieties, and electrophoretic karyotypes of clinical isolates of Cryptococcus neoformans from Brazil, Chile, and Venezuela

One hundred clinical isolates of Cryptococcus neoformans from human immunodeficiency virus (HIV)-infected and non-HIV-infected patients from Brazil, Chile, and Venezuela were separated according to varieties and tested for antifungal susceptibility. A high susceptibility to antifungal agents was observed among all the isolates. The electrophoretic karyotyping of 51 strains revealed good discrimination among Cryptococcus neoformans var. neoformans strains.3962348235

Human cryptococcosis: relationship of environmental and clinical strains of Cryptococcus neoformans var. neoformans from urban and rural areas

Forty-five clinical and 55 environmental strains of Cryptococcus neoformans var. neoformans from Sao Paulo, Brazil, were tested for their susceptibilities to amphotericin B, fluconazole, itraconazole, and flucytosine by the broth microdilution method according to the National Committee of Clinical Laboratory Standards guidelines. Electrophoretic karyotypes analysis by counter-clamped homogeneous electrophoresis was used to compare their genetic relatedness. Molecular typing revealed three clinical profiles very similar to two environmental profiles and an identical environmental and clinical pro. le. The results showed that human cryptococcosis can be acquired from environmental strains, which had similar minimum inhibitory concentration values to clinical strains, for antifungal agents.159171

Antifungal susceptibilities, varieties, and electrophoretic karyotypes of clinical isolates of Cryptococcus neoformans from Brazil, Chile and Venezuela

Artigo encontra-se disponível em acesso aberto no site do Editor.Submitted by Repositório Arca ([email protected]) on 2019-04-24T12:23:04Z No. of bitstreams: 1 license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5)Approved for entry into archive by Janaína Nascimento ([email protected]) on 2019-06-20T18:00:20Z (GMT) No. of bitstreams: 2 ve_Calvo_Belinda_etal_INI_2001.pdf: 212025 bytes, checksum: bf16ea470c4361b8dfa39222f8601db0 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5)Made available in DSpace on 2019-06-20T18:00:20Z (GMT). No. of bitstreams: 2 ve_Calvo_Belinda_etal_INI_2001.pdf: 212025 bytes, checksum: bf16ea470c4361b8dfa39222f8601db0 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2001Universidad del Zulia-Luz. Maracaibo, Venezuela.Clínica Santa María. Santiago, Chile.Clínica Santa María. Santiago, Chile.Hospital Clínico de Caracas, Caracas, Venezuela.Clínica Santa María, Santiago, ChileFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Universidade Federal de Paraná. Curitiba, PR, Brasil.Chiba University. Chiba, Japan.Chiba University. Chiba, Japan.Chiba University. Chiba, Japan.Chiba University. Chiba, Japan.Universidade Estadual de Campinas. Campinas, SP, Brasil.One hundred clinical isolates of Cryptococcus neoformans from human immunodeficiency virus (HIV)-infected and non-HIV-infected patients from Brazil, Chile, and Venezuela were separated according to varieties and tested for antifungal susceptibility. A high susceptibility to antifungal agents was observed among all the isolates. The electrophoretic karyotyping of 51 strains revealed good discrimination among Cryptococcus neoformans var. neoformans strains