2014 Dahlberg Award Winner: The effects of dietary toughness on occlusopalatal variation in savanna baboons

This study investigates the relationship between dietary toughness and craniofacial variation in two groups of savanna baboons. Standard craniofacial and malocclusion data were collected from a captive, soft-diet experiment group (n=24) and a sample of wild-captured baboons, raised on tougher, natural foods (n=19). We tested the hypothesis that in the absence of normal masticatory stress experienced during the consumption of wild foods, the captive baboons would exhibit higher levels of facial and dental structural irregularities. Principal component analysis indicates separation of the two samples. The soft-diet sample exhibits significantly shorter palates, greater variability in palate position, and higher frequencies of occlusal irregularities that correlate with the shorter palates. Results offer further support that long-term dietary chewing stresses have a measurable effect on adult craniofacial variation

BURIAL AND SOCIAL ORGANIZATION IN ITALIAN IRON AGE NECROPOLES: TESTING A BIODISTANCE APPROACH

This doctoral thesis examines complex burial behaviors as ritualized responses to changing sociopolitical landscapes just prior to a warring-states period and emergence of Rome as world power. A multivariate statistical approach investigates skeletal estimations of biological kinship (“biodistance”) and its role in the burial and social organizational practices of two central Italian Iron Age (1000-27 BC) groups: Pentri Samnites from Alfedena Campo Consolino (600-400 BC, L’Aquila, Abruzzo) and Pretuzi from Campovalano (750-100 BC, Teramo, Abruzzo). Despite missing data and sample imbalances, these are two of the largest, best-preserved, and generally contemporaneous Iron Age series spanning prehistoric, protohistoric, and historic periods. Alfedena Campo Consolino is a special subsection of a broader burial area and Campovalano represents a nearly complete necropolis. Most data from these Iron Age semi-transhumant agropastoralists comes from mortuary rather than settlement contexts. Thus, burial location is a central archaeological theme because of its potential to indicate corporate land ownership, group permanence, and identity. However, burial areas tended to be structured by family lineages and the similar material cultures they contain confound detailed discernment of the social identities encoded within the graves. I test the hypothesis that the mountainous and economically less-incorporated Pentri Samnites at Alfedena Campo Consolino will have stronger associations between biological and burial distances due to greater emphasis on biological kinship organization of the deceased. On the other hand, I expect that the Pretuzi from Campovalano will be more phenotypically variable as a result of broader ideas of kinship due to further economic and social reaches. To test these hypotheses, Mantel tests were used to examine the strength of association between biological similarity and spatial proximity of burials. Also, multidimensional scaling and univariate and multivariate analyses of variance were performed on data subgrouped by burial location, sex, time period, head position, and clothes brooch frequencies. Distribution of widely found funerary items, brooches, were examined in-depth for the potential that they varied spatially with biological patterns of variation as a marker of biological group membership. In general, I think brooches were well-made, distinctive, and highly visible indicators of wearers’ social position and identity. Male faces and cranial bases at Alfedena Campo Consolino and female multivariate tooth row measures at Campovalano produce the most noticeable signals. Because samples differ so greatly in their compositions and sizes, results of this study cannot specify if ACC was organized by biological kinship to a greater degree than CMV. Instead, results are interpreted in terms of the idea that a greater diversity of burial and social organization existed in Iron Age central Italy than previously thought. This research constitutes an important advance in evaluation of the spatial dimension of mortuary practices and social identity formation during an unstable time, and novel biodistance approaches such as those developed in this thesis should be considered as additional lines of evidence for comprehensive mortuary analyses

A Novel Ensemble Machine Learning Approach for Bioarchaeological Sex Prediction

I present a novel machine learning approach to predict sex in the bioarchaeological record. Eighteen cranial interlandmark distances and five maxillary dental metric distances were recorded from n = 420 human skeletons from the necropolises at Alfedena (600–400 BCE) and Campovalano (750–200 BCE and 9–11th Centuries CE) in central Italy. A generalized low rank model (GLRM) was used to impute missing data and Area under the Curve—Receiver Operating Characteristic (AUC-ROC) with 20-fold stratified cross-validation was used to evaluate predictive performance of eight machine learning algorithms on different subsets of the data. Additional perspectives such as this one show strong potential for sex prediction in bioarchaeological and forensic anthropological contexts. Furthermore, GLRMs have the potential to handle missing data in ways previously unexplored in the discipline. Although results of this study look promising (highest AUC-ROC = 0.9722 for predicting binary male/female sex), the main limitation is that the sexes of the individuals included were not known but were estimated using standard macroscopic bioarchaeological methods. However, future research should apply this machine learning approach to known-sex reference samples in order to better understand its value, along with the more general contributions that machine learning can make to the reconstruction of past human lifeways

Overview of attacks against civilian infrastructure during the Syrian civil war, 2012–2018

Hundreds of thousands of people have been killed during the Syrian civil war and millions more displaced along with an unconscionable amount of destroyed civilian infrastructure. We aggregate attack data from Airwars, Physicians for Human Rights and the Safeguarding Health in Conflict Coalition/Insecurity Insight to provide a summary of attacks against civilian infrastructure during the years 2012-2018. Specifically, we explore relationships between date of attack, governorate, perpetrator and weapon for 2689 attacks against five civilian infrastructure classes: healthcare, private, public, school and unknown. Multiple correspondence analysis (MCA) via squared cosine distance, k-means clustering of the MCA row coordinates, binomial lasso classification and Cramer's V coefficients are used to produce and investigate these correlations. Frequencies and proportions of attacks against the civilian infrastructure classes by year, governorate, perpetrator and weapon are presented. MCA results identify variation along the first two dimensions for the variables year, governorate, perpetrator and healthcare infrastructure in four topics of interest: (1) Syrian government attacks against healthcare infrastructure, (2) US-led Coalition offensives in Raqqa in 2017, (3) Russian violence in Aleppo in 2016 and (4) airstrikes on non-healthcare infrastructure. These topics of interest are supported by results of the k-means clustering, binomial lasso classification and Cramer's V coefficients. Findings suggest that violence against healthcare infrastructure correlates strongly with specific perpetrators. We hope that the results of this study provide researchers with valuable data and insights that can be used in future analyses to better understand the Syrian conflict

Potential clinical utility of ERC-2 yeast phase lysate antigen for antibody detection in dogs with blastomycosis

Four Blastomyces antigens ERC-2 (B. gilchristii, dog, Wisconsin), B5929 (human, Minnesota), 597 (human, Wisconsin), and T-27 (polar bear, Tennessee) were tested against 31 serum specimens from dogs with blastomycosis and 19 from healthy dogs. All antigens detected antibody; efficacy varied. ERC-2 showed the highest ELISA mean absorbance value of 3.00 followed by T-27. Test performance varied by sample geographic origin. Further study is needed to determine if ERC-2 antigens may be clinically useful, and whether the combination of the particular fungal species as antigen source, host animal, and the species and geographic location of the patient being tested is important for optimum test characteristics

Water and war: The effect of functioning chlorinated water stations in reducing waterborne diseases during conflict in northwest Syria, 2017-2021.

Since 2011, Syria has been engulfed in a complex conflict marked by both targeted and indiscriminate attacks on civilians and civilian infrastructure. Water infrastructure has been continuously targeted, exacerbating problems with contamination of and access to clean adequate drinking water, and increasing the risk of waterborne diseases. We aimed to determine whether having access to more functional and chlorinated water stations is associated with a reduced risk of waterborne disease in northwest Syria. We examined the effect of functioning chlorinated water stations on the incidence of waterborne disease in 10 districts of Northwest Syria between January 1, 2017, and June 30, 2021, using weekly reported disease surveillance data and data from a water, sanitation, and hygiene (WASH) system evaluation program of the Assistance Coordination Unit (ACU). We ran eight negative binomial models to examine the association between functioning chlorinated water stations and the incidence of four of the five waterborne diseases: acute bloody diarrhea (ABD), acute other diarrhea (AOD), acute jaundice syndrome (AJS), and severe typhoid fever (STF). Dose-response models were used to investigate how the incidence of disease can theoretically be reduced as functioning and chlorinated water stations strategically increase. Compared to areas with lower quintiles of functioning and chlorinated water stations, the rates of the four waterborne diseases were lower in areas with higher quintiles of functioning and chlorinated water stations. Exposure to functioning water stations had a stronger association with lower rates of waterborne diseases than exposure to chlorinated water stations. Dose-response models demonstrate a potential for curbing the incidence of acute diarrhea and acute jaundice syndrome. The results of this study provide an understanding of the effects of water station functionality and chlorination in conflict settings. These findings support greater prioritization of WASH activities in countries experiencing violence against civilian infrastructure

Water and war: The effect of functioning chlorinated water stations in reducing waterborne diseases during conflict in northwest Syria, 2017-2021.

Since 2011, Syria has been engulfed in a complex conflict marked by both targeted and indiscriminate attacks on civilians and civilian infrastructure. Water infrastructure has been continuously targeted, exacerbating problems with contamination of and access to clean adequate drinking water, and increasing the risk of waterborne diseases. We aimed to determine whether having access to more functional and chlorinated water stations is associated with a reduced risk of waterborne disease in northwest Syria. We examined the effect of functioning chlorinated water stations on the incidence of waterborne disease in 10 districts of Northwest Syria between January 1, 2017, and June 30, 2021, using weekly reported disease surveillance data and data from a water, sanitation, and hygiene (WASH) system evaluation program of the Assistance Coordination Unit (ACU). We ran eight negative binomial models to examine the association between functioning chlorinated water stations and the incidence of four of the five waterborne diseases: acute bloody diarrhea (ABD), acute other diarrhea (AOD), acute jaundice syndrome (AJS), and severe typhoid fever (STF). Dose-response models were used to investigate how the incidence of disease can theoretically be reduced as functioning and chlorinated water stations strategically increase. Compared to areas with lower quintiles of functioning and chlorinated water stations, the rates of the four waterborne diseases were lower in areas with higher quintiles of functioning and chlorinated water stations. Exposure to functioning water stations had a stronger association with lower rates of waterborne diseases than exposure to chlorinated water stations. Dose-response models demonstrate a potential for curbing the incidence of acute diarrhea and acute jaundice syndrome. The results of this study provide an understanding of the effects of water station functionality and chlorination in conflict settings. These findings support greater prioritization of WASH activities in countries experiencing violence against civilian infrastructure