Physiological studies of Sclerotium rolfsii Sacc. causing collar rot of peppermint

In vitro studies were conducted on the effect of temperature, pH levels, carbon, nitrogen and amino acids on the mycelial growth and biomass production of Sclerotium rofsii Sacc. causing collar rot of mint. The results reveal that the growth of S. rolfsii was maximum at 30°C which was reduced significantly below 20°C and above 35°C. Of the pH levels tested, acetic pH (5.0) produced maximum mycelial dry weight which was followed by exposing the pathogen to pH 6.0. Among the nine carbon sources tested, sucrose recorded the maximum mycelial growth and dry weight of S. rolfsii, while peptone was the best among the nitrogen sources and tryptophane and phenylalanine was the best amino acids on the mycelial growth and biomass production of S. rolfsii.Key words: Sclerotium rolfsii, pH, mint, collar rot, nutritional studies, temperature

Polymers pushing Polymers: Polymer Mixtures in Thermodynamic Equilibrium with a Pore

We investigate polymer partitioning from polymer mixtures into nanometer size cavities by formulating an equation of state for a binary polymer mixture assuming that only one (smaller) of the two polymer components can penetrate the cavity. Deriving the partitioning equilibrium equations and solving them numerically allows us to introduce the concept of "polymers-pushing-polymers" for the action of non-penetrating polymers on the partitioning of the penetrating polymers. Polymer partitioning into a pore even within a very simple model of a binary polymer mixture is shown to depend in a complicated way on the composition of the polymer mixture and/or the pore-penetration penalty. This can lead to enhanced as well as diminished partitioning, due to two separate energy scales that we analyse in detail.Comment: 10 pages, 6 figure

Assessing the in vitro efficacy of biocontrol agents and oil cakes against basal rot of onion incited by Fusarium oxysporum f.sp. cepae

Onions are an important vegetable crop, which is infected by many soils and foliar pathogens. Among them, Fusarium Basal Rot (FBR) causes yield losses of up to 50 per cent in the field and 30 to 40 per cent during post-harvest storage of bulbs.  For management of basal rot of onion, the efficacy of native antagonists such as six different Trichoderma sp. (T1-T6), five different Bacillus sp. (B1-B5) and five different oil cakes was assessed against the Fusarium oxysporum f.sp. cepae under in vitro condition. Among them, T3 collected from Kulithalai recorded maximum virulence as well as dark green sporulation with conidia length of 2.68–3.25 and breadth of 2.54-3.46µ. Among the tested isolates, In the case of  Bacillus sp., isolate B4 recorded the maximum inhibition zone (66.16%), followed by B. subtilis (B5), which recorded a (59.03%) inhibition on the mycelial growth. Among the five different oil cakes, the filtrates of neem cake showed a maximum inhibition zone against F. oxysporum f.sp. cepae of 1.29 cm @ 15% concentration, followed by groundnut cake at 1.36 cm @ 30% concentration. Hence the different control measures, Trichoderma sp. showed critically acclaimed performance under in vitro than others. The combined application of Trichoderma sp, Bacillus sp and neem oilcake significantly inhibited the growth of basal rot of onion due to the presence of the antimicrobial property.   

Anomalous Dynamics of Forced Translocation

We consider the passage of long polymers of length N through a hole in a membrane. If the process is slow, it is in principle possible to focus on the dynamics of the number of monomers s on one side of the membrane, assuming that the two segments are in equilibrium. The dynamics of s(t) in such a limit would be diffusive, with a mean translocation time scaling as N^2 in the absence of a force, and proportional to N when a force is applied. We demonstrate that the assumption of equilibrium must break down for sufficiently long polymers (more easily when forced), and provide lower bounds for the translocation time by comparison to unimpeded motion of the polymer. These lower bounds exceed the time scales calculated on the basis of equilibrium, and point to anomalous (sub-diffusive) character of translocation dynamics. This is explicitly verified by numerical simulations of the unforced translocation of a self-avoiding polymer. Forced translocation times are shown to strongly depend on the method by which the force is applied. In particular, pulling the polymer by the end leads to much longer times than when a chemical potential difference is applied across the membrane. The bounds in these cases grow as N^2 and N^{1+\nu}, respectively, where \nu is the exponent that relates the scaling of the radius of gyration to N. Our simulations demonstrate that the actual translocation times scale in the same manner as the bounds, although influenced by strong finite size effects which persist even for the longest polymers that we considered (N=512).Comment: 13 pages, RevTeX4, 16 eps figure

Quasiparticles as composite objects in the RVB superconductor

We study the nature of the superconducting state, the origin of d-wave pairing, and elementary excitations of a resonating valence bond (RVB) superconductor. We show that the phase string formulation of the t-J model leads to confinement of bare spinon and holon excitations in the superconducting state, though the vacuum is described by the RVB state. Nodal quasiparticles are obtained as composite excitations of spinon and holon excitations. The d-wave pairing symmetry is shown to arise from short range antiferromagnetic correlations

Anomalous Dynamics of Translocation

We study the dynamics of the passage of a polymer through a membrane pore (translocation), focusing on the scaling properties with the number of monomers $N$. The natural coordinate for translocation is the number of monomers on one side of the hole at a given time. Commonly used models which assume Brownian dynamics for this variable predict a mean (unforced) passage time $\tau$ that scales as $N^2$, even in the presence of an entropic barrier. However, the time it takes for a free polymer to diffuse a distance of the order of its radius by Rouse dynamics scales with an exponent larger than 2, and this should provide a lower bound to the translocation time. To resolve this discrepancy, we perform numerical simulations with Rouse dynamics for both phantom (in space dimensions $d=1$ and 2), and self-avoiding (in $d=2$) chains. The results indicate that for large $N$, translocation times scale in the same manner as diffusion times, but with a larger prefactor that depends on the size of the hole. Such scaling implies anomalous dynamics for the translocation process. In particular, the fluctuations in the monomer number at the hole are predicted to be non-diffusive at short times, while the average pulling velocity of the polymer in the presence of a chemical potential difference is predicted to depend on $N$.Comment: 9 pages, 9 figures. Submitted to Physical Review