Molekularno otkrivanje i genomska svojstva psećeg virusa Torque teno u Turskoj

Torque teno virus (TTV) was first detected in humans, and since then it has been reported in many host species, such as monkeys, cats, pigs, seagulls and dogs. The aim of this study was to investigate the presence of Torque teno canis virus (TTCaV) in shelter dogs housed in Sivas Municipal Animal Shelter, Turkey. Faecal specimens, including diarrheic and non-diarrheic (n=202), were collected from dogs of various age groups. In total, 32.18% (65/202) of samples were found positive for TTCaV. Out of the 65 positive samples, 34.64% (44/127) samples were from adult diarrheic dogs and 26.09% (6/23) belonged to diarrheic puppies. On the other hand, 28.84% (15/52) positive samples were detected from clinically healthy dogs. Eight sequences showed close homology among themselves, however, the sequences of two samples (CANEL130 and CANEL140) were genetically distinct from other published sequences. This is the first report on the detection of TTCaV in adult dogs and puppies in Turkey, and provides evidence that TTCaV cannot be considered as the sole cause of diarrhea.Virus Torque teno (TTV) otkriven je najprije u ljudi, no otada je zabilježen i u mnogim drugim domaćinima kao što su majmuni, mačke, svinje, galebovi i psi. Cilj ovoga rada bio je istražiti prisutnost psećeg virusa Torque teno (TTCaV) u pasa u azilu Sivas Municipal Animal Shelter u Turskoj. Uzorci izmeta (n = 202), uključujući one s proljevom i bez proljeva, prikupljeni su od pasa različitih dobnih skupina. Ukupno je 32,18 % (65/202) uzoraka bilo pozitivno na TTCaV. Od 65 pozitivnih uzoraka njih 34,64 % (44/127) potjecalo je od odraslih pasa s proljevom, a 26,09 % (6/23) od štenadi s proljevom. S druge strane, 28,84 % (15/52) pozitivnih uzoraka utvrđeno je u klinički zdravih pasa. Osam je sekvencija pokazalo međusobnu blisku srodnost, no sekvencije dvaju uzoraka (CANEL130 i CANEL140) genetski su se razlikovale od drugih sekvencija. Ovo je prvi nalaz TTCaV u odraslih pasa i štenadi u Turskoj i dokazuje da se TTCaV ne može smatrati jedinim uzročnikom proljeva

GENETIC DIVERSITY of OVINE HERPESVIRUS 2 STRAINS OBTAINED FROM MALIGNANT CATARRHAL FEVER CASES in EASTERN TURKEY

Malignant Catarrhal Fever (MCF) is a generalized, definitive lethal disease affecting the epithelial and lymphoid tissues of the respiratory and digestive tract, mainly cattle and some wild ruminants such as deer, buffalo or antelope. The sheep-related form of MCF is known to be present in Turkey and is caused by ovine herpesvirus 2 (OvHV-2). The aim of this study was to reveal the genetic diversity of OvHV-2 strains obtained from MCF cases in Eastern Turkey where the livestock industry has an important impact on economic activities. For this purpose, RTA (Replication and transcription activator), FGARAT (formylglycineamide ribotide amidotransferase) and some of glycoprotein genes (0v7, Ov8 ex2, ORF27 and Ov9.5) were investigated in blood samples from 24 cattles, clinically diagnosed with MCF. Genomic data of chosen samples were furthermore used to characterize and undergo combined phylogenetic analysis to determine possible alleles and subvariants. The results showed that high level of OvHV-2 diversity existed in selected genes and strains carrying allelic variants might circulate both in two geographically distinct regions and in a region itself. Moreover, three different OvHV-2 types and various subtypes were identified based on multi locus approach. This study provides important data to epidemiological research and thereby helps to determine the source of the virus and understand the spread of the disease

Case Report: Lupoid cutaneous leishmaniasis mimicking verruca plana

Cutaneous leishmaniasis (CL) is an infectious disease caused by various species of leishmania protozoan parasites. Lupoid CL is a rare form of CL that has a stunning similarity to other granulomatous cutaneous conditions of infectious or inflammatory origin. Verruca plana, also known as a "flat wart", is a benign proliferation of the skin resulting from infection with human papilloma virus (HPV). Herein, we presented a case of lupoid CL mimicking verruca plana on the face. © 2017 Ozlu E et al

The rapid effect of pulsed dye laser on demodex density of facial skin

WOS: 000465327400001PubMed: 29883220Background: Recently, treatment with acaricides, which is aimed at reducing excessive proliferation of demodex mites, has gained popularity due to its providing a significant improvement in the symptoms of diseases, such as rosacea, seborrhoeic dermatitis, and perioral dermatitis. The effect of IPL on demodex mites was reported in skin biopsy specimens in three patients; however, to the best of our knowledge, no study exists to date, which evaluates the effect of pulsed dye laser (PDL) on demodex density (Dd) in larger patient group. We aim here in to observe the Dd before and after PDL therapy with two different skin biopsy techniques. Material and methods: Thirty-one patients diagnosed with rosacea were included in the study who received PDL treatment. Dds which were measured by using both the SSSB (standardized skin surface biopsy) and CTM (cellophane tape method) techniques before and after 3 weeks of PDL therapy were evaluated. Results and discussion: The Dd of patients before PDL treatment was 13.0 (interquartile range (IQR): 5.0-28.0) and after 3 weeks of PDL treatment it was 6.0 (IQR: 3.0-12.0) with SSSB. After PDL treatment, the Dd was significantly lower than pretreatment the Dd (p = 0.002). The present study shows that PDL significantly reduced Dd in facial skin with one session