Potential Biological Control Agents against Soft Rot Diseases Caused by Pectobacteria on Some Sugar Beet Cultivars

Sugar beet is one of the most economically important agricultural crops cultivated in many provinces of Turkey. Especially in recent years, there has been an increase in bacterial tuber rot due to factors related to climate change. In preliminary trials, soft rot disease by Pectobacterium caratovorum subsp. caratovorum (Pcc) and Pectobacterium betavasculorum (Pb) were detected predominantly in sugar beets in Central Anatolia. Today, some cultural measures and copper compounds are used against soft rot agents in sugar beet, but successful results cannot be obtained in preventing the disease. In this study, a total of 270 soil samples were taken from the rhizosphere of 10 different fields in 3 different periods in 3 different ecologically diverse districts (Çumra, Altınekin and Seydişehir) of Konya, one of the provinces with the highest amount of sugar beet production in Turkey. As a result of the isolations, a total of 3064 bacterial isolates were purified and 262 of them showed antibacterial activity against Pcc and Pb in vitro conditions. In addition, 15 antagonist bacteria with the highest inhibitory effect on the development of both pathogens were tested in greenhouse conditions, and according to the results obtained from here, 3 antagonists with the highest effect were tested in field conditions in the cultivation areas of 3 different districts named above. Biochemical, morphological and molecular diagnoses of antagonist bacteria with high efficacy were made. According to the results obtained, it has been concluded that rhizospheric bacteria with antagonistic effect have a success rate of 33-90% against Pcc and Pb pathogens, and that the biological products to be prepared in future studies can be used in ecological, climate friendly and within sustainable agricultural practices in sugar beet production areas

Micropropagation of Adana and around naturally grown carob (ceratonia siliqua l.) genotypes with the classic and new generation tissue culture techniques and determination of genetic stability.

TEZ11373Tez (Yüksek Lisans) -- Çukurova Üniversitesi, Adana, 2018.Kaynakça (s. 79-85) var.xiv, 87 s. : res. (bzs. rnk.), tablo ; 29 cm.Bu çalışmada, Akdeniz Bölgesinde doğal olarak yayılım gösteren üç farklı keçiboynuzu genotipinin Plantform biyoreaktör sistemi ve katı besin ortamlarında karşılaştırmalı olarak mikroçoğaltım ve köklendirme çalışmaları yürütülmüştür. İlk olarak genotiplerin katı kültür mikroçoğaltım denemelerinde, MS, ½ MS ve WPM besin ortamları ile BA (0, 0.5, 1.0, 1.5, 2.0 mg/L) ve GA3 (0.1, 0.5 mg/L) bitki büyüme düzenleyicileri uygulanmıştır. Katı kültür köklenme denemeleri için MS, ½ MS ve WPM besin ortamları ile NAA (0, 1.0, 2.0 mg/L) ve IBA (0, 1.0, 2.0 mg/L) büyüme düzenleyicileri denenmiştir. En yüksek mikroçoğaltım değerleri, MS ve ½ MS besin ortamlarından ve 0.5 ve 2 mg/L BA+0.5 mg/L GA3 bitki büyüme düzenleyici konsantrasyonlarından elde edilmiştir. Mikroçoğaltım için belirlenen en iyi besin ortamı içeriği ile Plantform sisteminde çalışılmıştır. Çalışma neticesinde, Plantform sistemi her üç keçiboynuzu genotipi için bitki boyu, çoğalma katsayısı ve bitki kalitesi bakımından katı kültür sistemine göre daha iyi sonuç vermiştir. SSR markırları ile yapılan tarama sonucunda katı kültür ortam ı vePlantform sisteminde çoğaltılan bitkilerde herhangi bir genetik açılımın olmadığı tespit edilmiştir.In this study, micropropagation and rooting studies were carried out in comparative Plantform bioreactor system and solid media with three different carob genotypes which are growing naturally in the Mediterranean Region. First of all, micropropagation of carob genotyps were evaluated in solid MS, ½ MS and WPM media supplemented with BA (0, 0.5, 1.0, 1.5, 2.0 mg/L) and GA3 (0.1, 0.5 mg/L) for micropropagation, in MS, ½ MS and WPM media supplemented with IBA (0, 1.0, 2.0 mg/L) and NAA (0, 1.0, 2.0 mg/L) for rooting. Based on the solid media, the best results in all three genotypes were obtained from MS and ½ MS medium containing 0.5 and 2 mg/L BA+0.5 mg/L GA3 plant growth regulatory concentrations for micropropagation. Micropropagation studies were carried out in the Plantform system with the best-defined media content. As a result of studies, Plantform system showed better plant growth, multiplication coefficient and plant quality than solid culture system in all three genotypes propagation medium. Genetic stability of plants grown in solid culture and Plantform systems was tested by SSR markers

Investigation of The Antibacterial Effects of Different Plant Extracts Against Pea Bacterial Leaf Blight Disease Caused by Pseudomonas syringae pv. pisi

WOS: 000496182800003Pseudomonas syringae pv. pisi causing pea bacterial leaf blight disease in pea production areas all over the world is a seed-borne pathogen. The initial symptoms of the disease are observed as water-soaked stains on the cotyledon leaves and turn into necrotic stains at a further stage. In this study, the antibacterial properties of plant extracts has tested against Pseudomonas syringae pv. pisi. For this purpose, 34 different medicinal and aromatic plant extracts were investigated in vitro conditions by using diffusion disc method and the degree of inhibition on inoculum was determined through applying plant extracts that found effective to pathogen to seed before planting in the pot and field experiments. In the petri-experiments, nine plant species with antibacterial effects against the pathogen were identified. Three plant extracts (Allium sativum, Cistus creticus and Syzygium aromaticum) which showed the highest antibacterial effect in vitro experiments conducted by applicating of different doses; were investigated in terms of their impact on disease outbreak in pot and field experiments. These three plant extracts suppressed the disease in the pot experiment by %17-95, in the field experiment by %76-98. In this study, the most effective seed application was determined as Syzygium aromaticum. The extract suppressed the disease by 95% in pot experiments and 98% in the field experiments. In addition, Syzygium aromaticum extract had 5% germination enhancing effect. To conclude, the use of plant extracts as seed application in the integrated management of this disease has been considered as one of the successful management strategies