Upaya Pencegahan Koksidiosis Dengan Menggunakan Kandidat Vaksin Sporozoit Dan Protein Membran Sporozoit Eimeria Tenella = A prevention of coccidiosis using a vaccine candidate of eimeria tenella\u27s sporozoites and its...

Abstract The purpose of the research was to study of the effectivity of Eimeria tenella\u27s sporozites and its protein membrane as a vaccine candidate against coccidiosis. One hundred days old chickens (DOC) of the male layers (Isa Browns strain) were used as experimental animals, when those chickens were 4 days old, they were divided randomly into 5 groups (group of control, group I, II, III, and IV), each group contained of 20 chickens. The chickens of group I and group II were injected 2.1 mg/chicken of protein membrane of E. tenella\u27s sporozoites, group I intravenously and intracutaneously, respectively. The chicken of the group III was given orally with 700 E. tenella\u27s sporozoites and the group IV vaccinated by Coccivac-DR. The control group was not treated. Those chickens were kept according to their groups and given them non coccidiostat feed and drinking water ad libitum. On the 22nd day after treatment, all chickens were infected orally with 1500 infective oocyst of E. tenella as a challenge infection. The clinical signs data was collected on the 3rd day untul the 11th day of treatment. On the 5th day, 50% of chickens were killed to get the data of lesion scores. The number of oocyst of E. tenella per gram faeces were collected on the 4th day until the 11th day of treatment. The clinical signs was analyzed descriptively. The lesion scores was analyzed statistically by the Rank test method, and the number of oocyst per gram faeces was analyzed according to split plot design and orthogonal contrast. The result showed that injection of 2.1 mg protein membrane of E. tenella\u27s sporozoites intravenously and intracutaneously were protected the infection by 1500 infective oocyst of E. tenella. Nevertheless, the E. tenella\u27s sporozoites, as a vaccine candidate, could decrease significantly (P.. Keywords: coccidoisis--sporozoites--protein membrane of sporozoites--E. tenell

PENGARUH INJEKSI PROTEIN MEMBRAN SPOROZOIT Eimeria tenella TERHADAP INDEKS REPRODUKSI OOSISTA PADA AYAM = THE EFFECT OF INJECT/ON OF PROTEIN MEMBRANE SPOROZOITES TO REPRODUCTION INDEX OF Eimeria tenella\u27s OOCYST IN CHICK

Penelitian ini bertujuan untuk mengetahui pengaruh injeksi _protein membran sporozoit Eimeria tenella terhadap Indeks Reproduksi E. tenella pada ayam. Dalam penelitian ini, sebanyak 60 ekor DOC jantan petelur (strain Isa Brown) digunakan sebagai hewan percobaan. Ayam percobaan tersebut setelah berumur 4 hari dibagi acak menjadi tiga kelompok (kelompok kontrol, I dan II), masing-masina kelompok terdiri dari 20 ekor. Ayam-ayam pada kelompok I diinjeksi dengan protein membran sporozoit E tenella dengan dosis 2,1 jig per ekor secara intravena. Ayam-ayam pada kelompok [1. dinjeksi secara intrakutan dengan protein yang sama seperti yang diinjeksikan pada kelompok I, sedang ayam pada kelompok kontrol tidak dilakukan perlakuan. Semua ayam percobaan kemudian dimasukkan ke dalam kelompoknya masing-masing dan diberi pakan non koksidiostat dan air minum secara ad libitum. Pada hari ke-22 setelah perlakuan, semua ayam pereobaan diinfeksi dengan 1500 oosista iafektifE tenella per ekor sebagai infeksi tantangan. Data jumlah oosista per gram feses dikumpulkan mtdai hari ke-4 sarnpai dengan hari ke-11 setelah infeksi ta.ntangan. Hasil perhitungan Indeks Reproduksi dianalisis secara deskriptif Dari hasil penelitian ini dapat ditarilc ksimpulan bahwa injeksi 2,1 pg protein membran sporozoit E. tenella baik secara intravena maupun intrakutan tidak mempengaruhi Indeks Reproduksi El tenella pada ayam-ayam percobaan terhadap infeksi taniangan E. tenellcr. Kata kunci: Indeks Reproduksi, protein membran sporozoit, E tenell