Upaya Pencegahan Koksidiosis Dengan Menggunakan Kandidat Vaksin Sporozoit Dan Protein Membran Sporozoit Eimeria Tenella = A prevention of coccidiosis using a vaccine candidate of eimeria tenella\u27s sporozoites and its...

Abstract

Abstract The purpose of the research was to study of the effectivity of Eimeria tenella\u27s sporozites and its protein membrane as a vaccine candidate against coccidiosis. One hundred days old chickens (DOC) of the male layers (Isa Browns strain) were used as experimental animals, when those chickens were 4 days old, they were divided randomly into 5 groups (group of control, group I, II, III, and IV), each group contained of 20 chickens. The chickens of group I and group II were injected 2.1 mg/chicken of protein membrane of E. tenella\u27s sporozoites, group I intravenously and intracutaneously, respectively. The chicken of the group III was given orally with 700 E. tenella\u27s sporozoites and the group IV vaccinated by Coccivac-DR. The control group was not treated. Those chickens were kept according to their groups and given them non coccidiostat feed and drinking water ad libitum. On the 22nd day after treatment, all chickens were infected orally with 1500 infective oocyst of E. tenella as a challenge infection. The clinical signs data was collected on the 3rd day untul the 11th day of treatment. On the 5th day, 50% of chickens were killed to get the data of lesion scores. The number of oocyst of E. tenella per gram faeces were collected on the 4th day until the 11th day of treatment. The clinical signs was analyzed descriptively. The lesion scores was analyzed statistically by the Rank test method, and the number of oocyst per gram faeces was analyzed according to split plot design and orthogonal contrast. The result showed that injection of 2.1 mg protein membrane of E. tenella\u27s sporozoites intravenously and intracutaneously were protected the infection by 1500 infective oocyst of E. tenella. Nevertheless, the E. tenella\u27s sporozoites, as a vaccine candidate, could decrease significantly (P.. Keywords: coccidoisis--sporozoites--protein membrane of sporozoites--E. tenell