Spatial Modulation Microscopy for Real-Time Imaging of Plasmonic Nanoparticles and Cells

Spatial modulation microscopy is a technique originally developed for quantitative spectroscopy of individual nano-objects. Here, a parallel implementation of the spatial modulation microscopy technique is demonstrated based on a line detector capable of demodulation at kHz frequencies. The capabilities of the imaging system are shown using an array of plasmonic nanoantennas and dendritic cells incubated with gold nanoparticles.Comment: 3 pages, 4 figure

A Paraconsistent Higher Order Logic

Classical logic predicts that everything (thus nothing useful at all) follows from inconsistency. A paraconsistent logic is a logic where an inconsistency does not lead to such an explosion, and since in practice consistency is difficult to achieve there are many potential applications of paraconsistent logics in knowledge-based systems, logical semantics of natural language, etc. Higher order logics have the advantages of being expressive and with several automated theorem provers available. Also the type system can be helpful. We present a concise description of a paraconsistent higher order logic with countable infinite indeterminacy, where each basic formula can get its own indeterminate truth value (or as we prefer: truth code). The meaning of the logical operators is new and rather different from traditional many-valued logics as well as from logics based on bilattices. The adequacy of the logic is examined by a case study in the domain of medicine. Thus we try to build a bridge between the HOL and MVL communities. A sequent calculus is proposed based on recent work by Muskens.Comment: Originally in the proceedings of PCL 2002, editors Hendrik Decker, Joergen Villadsen, Toshiharu Waragai (http://floc02.diku.dk/PCL/). Correcte

Optimal Concentration of Light in Turbid Materials

In turbid materials it is impossible to concentrate light into a focus with conventional optics. Recently it has been shown that the intensity on a dyed probe inside a turbid material can be enhanced by spatially shaping the wave front of light before it enters a turbid medium. Here we show that this enhancement is due to concentration of light energy to a spot much smaller than a wavelength. We focus light on a dyed probe sphere that is hidden under an opaque layer. The light is optimally concentrated to a focus which does not exceed the smallest focal area physically possible by more than 68%. A comparison between the intensity enhancements of both the emission and excitation light supports the conclusion of optimal light concentration.Comment: We corrected an ambiguous description of the focus size in our abstract and text pointed out by an anonymous refere

Hyperspectral darkfield microscopy of single hollow gold nanoparticles for biomedical applications

Hyperspectral microscopy is a versatile method for simultaneous spatial and spectroscopic characterization of nonfluorescent samples. Here we present a hyperspectral darkfield imaging system for spectral imaging of single nanoparticles over an area of 150 × 150 µm2 and at illumination intensities compatible with live cell imaging. The capabilities of the system are demonstrated using correlated transmission electron microscopy and single-particle optical studies of colloidal hollow gold nanoparticles. The potential of the system for characterizing the interactions between nanoparticles and cells has also been demonstrated. In this case, the spectral information proves a useful improvement to standard darkfield imaging as it enables differentiation between light scattered from nanoparticles and light scattered from other sources in the cellular environment. The combination of low illumination power and fast integration times makes the system highly suitable for nanoparticle tracking and spectroscopy in live-cell experiments

Directional emission of light from a nano-optical Yagi-Uda antenna

The plasmon resonance of metal nanoparticles can enhance and direct light from optical emitters in much the same way that radio frequency (RF) antennas enhance and direct the emission from electrical circuits. In the RF regime, a typical antenna design for high directivity is the Yagi-Uda antenna, which basically consists of a one-dimensional array of antenna elements driven by a single feed element. Here, we present the experimental demonstration of directional light emission from a nano-optical Yagi-Uda antenna composed of an array of appropriately tuned gold nanorods. Our results indicate that nano-optical antenna arrays are a simple but efficient tool for the spatial control of light emission.Comment: 4 pages, including 4 figure

Component-Based Real-Time Operating System for Embedded Applications

Acceptance rate: 37%, Rank (CORE): AInternational audienceAs embedded systems must constantly integrate new functionalities, their developement cycles must be based on high-level abstractions, making the software design more flexible. CBSE provides an approach to these new requirements. However, low-level services provided by operating systems are an integral part of embedded applications, furthermore deployed on resource-limited devices. Therefore, the expected benefits of CBSE must not impact on the constraints imposed by the targetted domain, such as memory footprint, energy consumption, and execution time. In this paper, we present the componentization of a legacy industry-established Real-Time Operating System, and how component-based applications are built on top of it. We use the Think framework that allows to produce flexible systems while paying for flexibility only where desired. Performed experimentions show that the induced overhead is negligeable