296 research outputs found
Integrative analysis of the colorectal cancer proteome : potential clinical impact
Peer reviewedPostprin
A Comprehensive Study of Extramural Venous Invasion in Colorectal Cancer
Peer reviewedPublisher PD
Proteomics for early detection of colorectal cancer : recent updates
Funding: This manuscript was not funded.Peer reviewedPostprin
Recent advances in understanding the roles of matrix metalloproteinases in tumour invasion and metastasis
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The expression and prognostic significance of retinoic acid metabolising enzymes in colorectal cancer
Peer reviewedPublisher PD
Increased lymph node yield in colorectal cancer is not necessarily associated with a greater number of lymph node positive cancers
Peer reviewedPublisher PD
Metabolically active cytochrome P450 CYP1B1 in solid tumours: a novel target for chemotherapeutic intervention.
Cytochrome P450 CYP1B1 is a member of a superfamily of haemoproteins that are central to the oxidative metabolism of a wide variety of endogenous and exogenous compounds. Several of these enzymes have an established role in the metabolic bio-transformation of a variety of anti-cancer drugs. In this study, we demonstrated both CYP1B1 and CPR activity in the microsomal fraction of ovarian and kidney tumours
Lack of association between the rs2294008 polymorphism in the prostate stem cell antigen gene and colorectal neoplasia : a case-control and immunohistochemical study
PMID: 22824379 [PubMed - indexed for MEDLINE] PMCID: PMC3500224 Free PMC ArticlePeer reviewedPublisher PD
The Proteomics of Colorectal Cancer: Identification of a Protein Signature Associated with Prognosis
Colorectal cancer is one of the commonest types of cancer and there is requirement for the identification of prognostic biomarkers. In this study protein expression profiles have been established for colorectal cancer and normal colonic mucosa by proteomics using a combination of two dimensional gel electrophoresis with fresh frozen sections of paired Dukes B colorectal cancer and normal colorectal mucosa (nβ=β28), gel image analysis and high performance liquid chromatographyβtandem mass spectrometry. Hierarchical cluster analysis and principal components analysis showed that the protein expression profiles of colorectal cancer and normal colonic mucosa clustered into distinct patterns of protein expression. Forty-five proteins were identified as showing at least 1.5 times increased expression in colorectal cancer and the identity of these proteins was confirmed by liquid chromatographyβtandem mass spectrometry. Fifteen proteins that showed increased expression were validated by immunohistochemistry using a well characterised colorectal cancer tissue microarray containing 515 primary colorectal cancer, 224 lymph node metastasis and 50 normal colonic mucosal samples. The proteins that showed the greatest degree of overexpression in primary colorectal cancer compared with normal colonic mucosa were heat shock protein 60 (p<0.001), S100A9 (p<0.001) and translationally controlled tumour protein (p<0.001). Analysis of proteins individually identified 14-3-3Ξ² as a prognostic biomarker (Ο2β=β6.218, pβ=β0.013, HRβ=β0.639, 95%CI 0.448β0.913). Hierarchical cluster analysis identified distinct phenotypes associated with survival and a two-protein signature consisting of 14-3-3Ξ² and aldehyde dehydrogenase 1 was identified as showing prognostic significance (Ο2β=β7.306, pβ=β0.007, HRβ=β0.504, 95%CI 0.303β0.838) and that remained independently prognostic (pβ=β0.01, HRβ=β0.416, 95%CI 0.208β0.829) in a multivariate model
Characterisation of the oxysterol metabolising enzyme pathway in mismatch repair proficient and deficient colorectal cancer
ACKNOWLEDGMENTS The immunohistochemistry was performed with the support of the Grampian Biorepository. GRANT SUPPORT Rebecca Swan was supported by the Jean Shanks Foundation. This study was supported by funding from Friends of Anchor and the Encompass kick start and SMART:Scotland award schemes of Scottish Enterprise.Peer reviewedPublisher PD
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