Unravelling plant responses to stress—the importance of targeted and untargeted metabolomics

Climate change and an increasing population, present a massive global challenge with respect to environmentally sustainable nutritious food production. Crop yield enhancements, through breeding, are decreasing, whilst agricultural intensification is constrained by emerging, re-emerging, and endemic pests and pathogens, accounting for ~30% of global crop losses, as well as mounting abiotic stress pressures, due to climate change. Metabolomics approaches have previously contributed to our knowledge within the fields of molecular plant pathology and plant–insect interactions. However, these remain incredibly challenging targets, due to the vast diversity in metabolite volatility and polarity, heterogeneous mixtures of pathogen and plant cells, as well as rapid rates of metabolite turn-over. Unravelling the systematic biochemical responses of plants to various individual and combined stresses, involves monitoring signaling compounds, secondary messengers, phytohormones, and defensive and protective chemicals. This demands both targeted and untargeted metabolomics approaches, as well as a range of enzymatic assays, protein assays, and proteomic and transcriptomic technologies. In this review, we focus upon the technical and biological challenges of measuring the metabolome associated with plant stress. We illustrate the challenges, with relevant examples from bacterial and fungal molecular pathologies, plant–insect interactions, and abiotic and combined stress in the environment. We also discuss future prospects from both the perspective of key innovative metabolomic technologies and their deployment in breeding for stress resistance

The molecular genetics of race 2 specificity in Pseudomonas syringae pathovar pisi

SIGLEAvailable from British Library Document Supply Centre- DSC:DX170190 / BLDSC - British Library Document Supply CentreGBUnited Kingdo

Arts Faculty Research Performance Day 2008: Digital Research

Research Performance Day showcased - in a series of ten minute talks - the richness and diversity of research in the Faculty, on the part of young scholars, established researchers, and postgraduates just embarking on an academic career. There were four panels: Digital scholarship in the Faculty; Peace and conflict; Postgraduate showcase and Histories of emotion. This year the Organising Committee invited researchers in the Faculty to present their digital research outputs. The DIU prepared a slide show with snapshots from digital publications to present a range of digital research outputs in the Faculty.Faculty of Arts, Digital Innovation Uni

Nitric oxide in plants: an assessment of the current state of knowledge

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Spectroscopic monitoring of NO traces in plants and human breath: applications and perspectives

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Dampak penerimaan sektor pajak terhadap peningkatan pendapatan asli daerah (PAD) di kabupaten Aceh Besar

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