A note on the use of FTA™ technology for storage of blood samples for DNA analysis and removal of PCR inhibitors

peer-reviewedFTA™ technology is widely used across many molecular disciplines for sample capture, storage and analysis. The use of this technology for the long-term storage of blood samples for DNA analysis was examined as well as its potential to remove inhibitors from DNA samples previously extracted from blood with PCR inhibitors remaining. It was found that blood spots stored on FTA™ cards for 8 years at room temperature gave successful PCR products and that FTA™ cards are a useful tool for removing substances in samples which interfere with or inhibit, the PCR reaction

Investigation of Prolific Sheep from UK and Ireland for Evidence on Origin of the Mutations in BMP15 (FecXG, FecXB) and GDF9 (FecGH) in Belclare and Cambridge Sheep

peer-reviewedThis paper concerns the likely origin of three mutations with large effects on ovulation rate identified in the Belclare and Cambridge sheep breeds; two in the BMP15 gene (FecXG and FecXB) and the third (FecGH) in GDF9. All three mutations segregate in Belclare sheep while one, FecXB, has not been found in the Cambridge. Both Belclare and Cambridge breeds are relatively recently developed composites that have common ancestry through the use of genetic material from the Finnish Landrace and Lleyn breeds. The development of both composites also involved major contributions from exceptionally prolific ewes screened from flocks in Ireland (Belclare) and Britain (Cambridge) during the 1960s. The objective of the current study was to establish the likely origin of the mutations (FecXG, FecXB and FecGH) through analysis of DNA from Finnish Landrace and Lleyn sheep, and Galway and Texel breeds which contributed to the development of the Belclare breed. Ewes with exceptionally high prolificacy (hyper-prolific ewes) in current flocks on Irish farms were identified to simulate the screening of ewes from Irish flocks in the 1960s. DNA was obtained from: prolific ewes in extant flocks of Lleyn sheep (n = 44) on the Lleyn peninsula in Wales; hyper-prolific ewes (n = 41); prolific Galway (n = 41) ewes; Finnish Landrace (n = 124) and Texel (n = 19) ewes. The FecXG mutation was identified in Lleyn but not in Finnish Landrace, Galway or Texel sheep; FecXB was only found among the hyper-prolific ewes. The FecGH mutation was identified in the sample of Lleyn sheep. It was concluded from these findings that the Lleyn breed was the most likely source of the FecXG and FecGH mutations in Belclare and Cambridge sheep and that the FecXB mutation came from the High Fertility line that was developed using prolific ewes selected from commercial flocks in Ireland in the 1960′s and subsequently used in the genesis of the Belclare.Financial support through the Teagasc Walsh Fellowship Scheme, Genesis Faraday SPARK award (Lleyn survey) and Science Foundation Ireland (07/SRC/B1156) is gratefully acknowledged

The Proposed 1972 Constitution for the State of Montana

This paid advertising supplement includes the text of the proposed 1972 constitution for the State of Montana as well as introduction and an article-by-article analysis. The supplement was distributed in several Montana newspapers.https://scholarworks.umt.edu/montanaconstitution/1047/thumbnail.jp

Anxiety and motor performance: More evidence for the effectiveness of holistic process goals as a solution to the process goal paradox

Objectives. Recent research has reported the benefits of using holistic rather than part process goals to avoid the negative effects associated with the conscious processing of task relevant information by skilled but anxious athletes. This experiment compared the efficacy of these two goal focus strategies in a neutral condition and a competitive condition in which cognitive state anxiety was elevated. Design. Laboratory-based experimental design using a mixed model with between (process goal groups) and within-subjects (neutral and competitive) conditions. Method. Thirty male and female undergraduate students aged between 19 and 44 years of age completed 896 practice repetitions of a race car driving simulation using discovery learning. Participants were then placed in either a holistic or part process goal group using stratified random assignment. The practice phase was followed by neutral and competitive conditions, during which driving performance and psychophysiological measures were collected. Results. Analysis of variance of lap times and driving errors revealed that the holistic process goal group outperformed the part process goal group in the competition condition. Analysis of psychophysiological measures suggested that the performance of both process goal groups in the competitive condition was associated with the investment of compensatory effort. Conclusions. Compared to part process goals, holistic process goals confer performance benefits for skilled athletes who perform under competitive pressure.The Leverhulme Trus

Integrating environmental awareness and sustainability issues in a beginning agronomy course

Agriculture in the United States in a science- based, highly technical field that has signifi­ cant impact on society and the larger ecosys­ tem, both nationally and internationally. As new technologies and challenges continually emerge, educational and research efforts in agriculture have been and will continue to be key to the success of agronomic systems. At the same time, human labor in U.S. agriculture has decreased rapidly; less than 2% of the population is directly involved in agricultural production. Rapid technological change and declining farm populations suggest that we are becoming a nation largely illiterate in agricul­ tural and sustainable production issues

Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 1. High abundance proteins, acute phase proteins and peptidomics

A peptidomic investigation of milk from an experimental model of Streptococcus uberis mastitis in dairy cows has incorporated a study of milk high abundance and acute phase (APP) proteins as well as analysis of low molecular weight peptide biomarkers. Intramammary infection (IMI) with S. uberis caused a shift in abundance from caseins, β-lactoglobulin and α-lactalbumin to albumin, lactoferrin and IgG with the increase in lactoferrin occurring last. The APP response of haptoglobin, mammary associated serum amyloid A3 and C-reactive protein occurred between 30–48 hours post challenge with peak concentrations of APPs at 72–96 hours post challenge and declined thereafter at a rate resembling the fall in bacterial count rather than the somatic cell count. A peptide biomarker panel for IMI based on capillary electrophoresis and mass spectrometry was developed. It comprised 77 identified peptides (IMI77) composed mainly of casein derived peptides but also including peptides of glycosylation dependent cell adhesion molecule and serum amyloid A. The panel had a biomarker classification score that increased from 36 hour to 81 hour post challenge, significantly differentiating infected from non-infected milk, thus suggesting potential as a peptide biomarker panel of bovine mastitis and specifically that of S. uberis origin. The use of omic technology has shown a multifactorial cross system reaction in high and low abundance proteins and their peptide derivatives with changes of over a thousand fold in analyte levels in response to S. uberis infection

Management of steers with various frame sizes on different nutritional levels

Call number: LD2668 .T4 ASI 1988 M84Master of ScienceAnimal Sciences and Industr