Editorial

No AbstractPeer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/56178/1/7_ftp.pd

Toward better measurement of sustainable development and wellbeing: a small number of SDG indicators reliably predict life satisfaction

In 2015, all 193 member states of the United Nations (UN) adopted the Sustainable Development Goals (SDGs). These 17 goals, 169 targets, and 232 indicators (including over 650 indicators if all the subdivisions are included) are intended to guide and improve sustainable wellbeing and life satisfaction for everyone on earth. Challenges include the fact that many indicators are not measured or reliably tracked in many countries, the cost of tracking is unclear, and no explicit overarching goal exists. To highlight some of the problems with this approach, we model life satisfaction(LS) survey scores by country, as a proxy for overall wellbeing, as the dependent variable against the official 232 SDG indicators. Using a constrained linear regression approach (LASSO), we identify a model that includes only 8 of the 232 indicators and explains 84% of the variation in LS. These eight indicators are proxies for economic, social, and environmental variables. We also cluster countries according to these indicators and LS showing correlation within geographical and cultural regions. We discuss these results with regard to the meaning and measurement of sustainable development vs. sustainable wellbeing and its relationship with LS and the SDGs. We recommend how these results can be used to prioritize goals and measurement efforts to create more meaningful and useful measures of sustainable wellbein

The global value of coastal wetlands for storm protection

Coastal wetlands provide a range of valuable ecosystem services, including protecting coastal communities from storms. We estimated for the first time the global value of these storm protection services for all coastal wetlands for both damages avoided and lives saved. We used the historical tracks of 1,014 tropical cyclones since 1902 that recorded property damage and/or human casualties in 71 countries/regions. We used Bayesian and OLS statistical techniques to relate storm damages and lives lost to: wind speed, storm forward speed, the year of the storm, the volume of ocean water proximal to landfall, and GDP, population, and coastal wetlands in the swath of the storm. Based on current storm probabilities, we estimate the median annual global value of coastal wetlands for storm protection at $447 billion/yr (2015$US) ($213 -$837 billion/yr, 90% CI) and 4,620 lives saved per year (3,320 – 6,550, 90% CI). The 40 million hectares of coastal wetlands in storm prone areas provided an average of $11,000/ha/yr in avoided storm damages. The frequency and intensity of tropical cyclones has been increasing in recent decades and is projected to further increase with climate change. Consequently, the already significant benefits from protecting and restoring coastal wetlands will become increasingly important and valuable in the future. These results justify much larger investments in conservation and restoration of coastal wetlands

Polarized Signatures of a Habitable World: Comparing Models of an Exoplanet Earth with Visible and Near-infrared Earthshine Spectra

In the JWST, Extremely Large Telescopes, and LUVOIR era, we expect to characterize a number of potentially habitable Earth-like exoplanets. However, the characterization of these worlds depends crucially on the accuracy of theoretical models. Validating these models against observations of planets with known properties will be key for the future characterization of terrestrial exoplanets. Due to its sensitivity to the micro- and macro-physical properties of an atmosphere, polarimetry will be an important tool that, in tandem with traditional flux-only observations, will enhance the capabilities of characterizing Earth-like planets. In this paper we benchmark two different polarization-enabled radiative-transfer codes against each other and against unique linear spectropolarimetric observations of the earthshine that cover wavelengths from $\sim$0.4 to $\sim$2.3 $\mu$m. We find that while the results from the two codes generally agree with each other, there is a phase dependency between the compared models. Additionally, with our current assumptions, the models from both codes underestimate the level of polarization of the earthshine. We also report an interesting discrepancy between our models and the observed 1.27 $\mu$m $O_2$ feature in the earthshine, and provide an analysis of potential methods for matching this feature. Our results suggest that only having access to the 1.27 $\mu$m $O_2$ feature coupled with a lack of observations of the $O_2$ A and B bands could result in a mischaracterization of an Earth-like atmosphere. Providing these assessments is vital to aid the community in the search for life beyond the solar system.Comment: 24 pages, 18 figure

Covid-19 pandemic impact on colonoscopy service and suggestions for managing recovery

Abstract Background and aim As the post-peak phase of the epidemic is approaching, there is an urgent need of an action plan to help resume endoscopy activity. To manage the Covid-19 pandemic-imposed backlog of postponed colonoscopy examinations, an efficient approach is needed. The practice of on-demand sedation with benzodiazepines and/or opiates will allow most patients to complete a water-aided examination with minimal or no sedation. Other methods reported to minimize patient discomfort during colonoscopy can be used, in addition to water-aided techniques. Unsedated or minimally sedated patients who do not require recovery or require a shorter one allow rapid turnaround. The practice obviates the need for assistance with deep sedation from anesthesiologists, who may be in short supply. Trainee education in water-aided colonoscopy has been demonstrated to confer benefits. This review provides some insights into the impact of Covid-19 on endoscopy services, challenges ahead, and possible solutions to help recovery of colonoscopy work and training

Far Ultraviolet Observations of Molecular Hydrogen in the Diffuse Interstellar Medium of Starburst Galaxies

(Abridged) We have used the Far Ultraviolet Spectroscopic Explorer (FUSE) to search for H_2 absorption in five starburst galaxies: NGC 1705, NGC 3310, NGC 4214, M83 (NGC 5236), and NGC 5253. We tentatively detect weak absorption by H_2 in M83 and NGC 5253, and set upper limits on the H_2 column density in the other galaxies. Conservative upper limits on the mass of molecular gas detected with FUSE are many orders of magnitude lower than the H_2 mass inferred from CO emission. This indicates that almost all of the H_2 is in the form of clouds with N(H_2)>10^20 cm^-2 that are opaque to far-UV light and therefore cannot be probed with far-UV absorption measurements. The far-UV continuum visible in the FUSE spectra passes between the dense clouds, which have a covering factor <1. The complex observational biases related to varying extinction across the extended UV emission in the FUSE apertures prevent an unambiguous characterization of the diffuse H_2 in these starbursts. However, the evidence is suggestive that there is less H_2 in the diffuse interstellar medium between the dense clouds compared to similarly reddened sight lines in the Milky Way. This holds with the expectation that the destructive UV radiation field is stronger in starbursts. However, previous UV observations of these starbursts have shown that there is reddening caused by the diffuse interstellar medium. This suggests that while diffuse H_2 may be destroyed in the starburst, dust still exists.Comment: 10 pages, 8 figures. Accepted by ApJ. For higher resolution versions of figures 1-6 please contact the primary autho

Complete genome sequence of methicillin-sensitive Staphylococcus aureus containing a heterogeneic staphylococcal cassette chromosome element

Staphylococcus aureus is a common human bacterium that sometimes becomes pathogenic, causing serious infections. A key feature of S. aureus is its ability to acquire resistance to antibiotics. The presence of the staphylococcal cassette chromosome (SCC) element in serotypes of S. aureus has been confirmed using multiplex PCR assays. The SCC element is the only vector known to carry the mecA gene, which encodes methicillin resistance in S. aureus infections. Here, we report the genome sequence of a novel methicillin-sensitive S. aureus (MSSA) strain: SCC-like MSSA463. This strain was originally erroneously serotyped as methicillin-resistant S. aureus in a clinical laboratory using multiplex PCR methods. We sequenced the genome of SCC-like MSSA463 using pyrosequencing techniques and compared it with known genome sequences of other S. aureus isolates. An open reading frame (CZ049; AB037671) was identified downstream of attL and attR inverted repeat sequences. Our results suggest that a lateral gene transfer occurred between S. aureus and other organisms, partially changing S. aureus infectivity. We propose that attL and attR inverted repeats in S. aureus serve as frequent insertion sites for exogenous genes.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000316747000011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701BiologySCI(E)PubMed0ARTICLE3268-2745

World regional differences in outcomes for patients with peripheral artery disease: Insights from the EUCLID trial

Regional variations exist in the epidemiology of peripheral artery disease (PAD), in comorbidities, use of secondary prevention, and outcomes. Large studies of these variations in worldwide populations are rare. The EUCLID (Examining Use of tiCagreLor In peripheral artery Disease) trial included 13,885 patients with PAD from four geographical regions (Central/South America, Europe, Asia, North America) and compared monotherapy with ticagrelor and clopidogrel. Inclusion criteria were either an ankle-brachial index < 0.80 or a prior revascularization. The primary efficacy endpoint was time to first occurrence of any event in the composite of cardiovascular death, myocardial infarction, or ischemic stroke and did not differ between the study arms. This post hoc analysis of EUCLID confirmed that regional differences occurred in the inclusion criteria with more prior revascularization in North America (73.9%) and Asia (72.5%) compared with Central/South America (34.0%) and Europe (51.6%). The characteristics of patients also differed. Prior amputation at baseline was most frequent in Central/South America (6.3%) compared with other regions (1.6-2.8%). A history of stroke was most common in Asia, coronary heart disease in North America, and diabetes in Central/South America compared with other regions. The incidence of outcomes in patients with PAD varied by region. North America had the highest rate of the primary combined endpoint (5.97 events/100 patient-years). Corresponding rates were 4.80, 3.95, and 3.87 for Asia, Europe, and Central/South America, respectively. Hospitalization for acute limb ischemia (events/100 patient-years) was most frequent in Europe (0.75) and North America (0.74) compared with Asia (0.60) and Central/South America (0.33). Adjustment for inclusion criteria and relevant PAD characteristics did not have a major impact on these regional differences. Further adjustment for concomitant disease, risk factors, and preventive medication modified the regional differences only marginally. In conclusion, substantial regional differences were found in cardiovascular and limb outcomes in patients with PAD and were not explained by variation in the category of included patients, concomitant disease, risk factors, and prevention. Such differences, which may be due to variation in other factors such as background population rates or clinical care, need to be considered when designing and interpreting large international studies (ClinicalTrials.gov Identifier: NCT01732822)

Quantitative Assessment of the Sensitivity of Various Commercial Reverse Transcriptases Based on Armored HIV RNA

The in-vitro reverse transcription of RNA to its complementary DNA, catalyzed by the enzyme reverse transcriptase, is the most fundamental step in the quantitative RNA detection in genomic studies. As such, this step should be as analytically sensitive, efficient and reproducible as possible, especially when dealing with degraded or low copy RNA samples. While there are many reverse transcriptases in the market, all claiming to be highly sensitive, there is need for a systematic independent comparison of their applicability in quantification of rare RNA transcripts or low copy RNA, such as those obtained from archival tissues.We performed RT-qPCR to assess the sensitivity and reproducibility of 11 commercially available reverse transcriptases in cDNA synthesis from low copy number RNA levels. As target RNA, we used a serially known number of Armored HIV RNA molecules, and observed that 9 enzymes we tested were consistently sensitive to ∼1,000 copies, seven of which were sensitive to ∼100 copies, while only 5 were sensitive to ∼10 RNA template copies across all replicates tested. Despite their demonstrated sensitivity, these five best performing enzymes (Accuscript, HIV-RT, M-MLV, Superscript III and Thermoscript) showed considerable variation in their reproducibility as well as their overall amplification efficiency. Accuscript and Superscript III were the most sensitive and consistent within runs, with Accuscript and Superscript II ranking as the most reproducible enzymes between assays.We therefore recommend the use of Accuscript or Superscript III when dealing with low copy number RNA levels, and suggest purification of the RT reactions prior to downstream applications (eg qPCR) to augment detection. Although the results presented in this study were based on a viral RNA surrogate, and applied to nucleic acid lysates derived from archival formalin-fixed paraffin embedded tissue, their relative performance on RNA obtained from other tissue types may vary, and needs future evaluation