ISOLASI DAN KARAKTERISASI GENUS BAKTERI ASAM LAKTAT DARI FERMENTASI DAGING BUAH PISANG KEPOK (Musa paradisiaca L.)

Lactic Acid Bacteria (LAB) are bacteria that are beneficial for the health of the body by improving the balance of intestinal microflora. Lactic acid bacteria can be isolated from fruits and vegetables, including kepok bananas (Musa paradisiaca L.). Kepok bananas have a higher carbohydrate content than other bananas, which is 27%. This study aims to determine the characteristics of the genus of lactic acid bacteria isolated from fermented kepok bananas (Musa paradisiacal L.). Isolation technique was carried out by pouring method and using MRS Broth and MRS Agar selective media. Identification of lactic acid bacteria isolates macroscopically and microscopically with gram staining and biochemical tests. The results obtained were 5 (five) isolates of lactic acid bacteria, namely BALF1, BALF2, BALF3, BALF4, and BALF5 which had the same morphological and biochemical characters as members of the genus Lactobacillus

PERTUMBUHAN BIJI KAKAO (Theobroma cacao L.) DENGAN PERENDAMAN DALAM BAWANG MERAH (Allium cepa L.) DAN LARUTAN GIBERELIN (GA3)

Cocoa beans (Theobroma cacao L.) are recalcitrant beans. Soaking cocoa beans in a solution containing growth regulators will accelerate the softening of the seed coat so that water from the solution will enter the beans imbibitably and accelerate the growth of the beans. The purpose of this study was to determine the effect of soaking onion extract (Allium cepa L.) and gibberellin (GA3) solution. on the growth of cocoa beans (T. cacao L). This study used a completely randomized design (CRD) with different concentrations of onion extract (B) and gibberellins (G) consisting of a combination of 0 ppm onion extract and 0 ppm GA3, a combination of 100 ppm red onion extract and 0 ppm GA3 extract, and a combination of 100 ppm red onion extract and 0 ppm GA3 extract. onion 0 ppm and GA3 100 ppm, a combination of onion extract 100 ppm and GA3 100 ppm, a combination of onion extract 100 ppm and GA3 200 ppm, a combination of onion extract 200 ppm and GA3 100 ppm treatment with 4 replications to obtain 24 experimental units. The results showed that soaking cocoa beans using a combination of onion extract and gibberellins resulted in 100% germination percentage and significantly affected plant height, wet weight and dry weight but had no significant effect on leaf number. The combination treatment of 100 ppm red onion extract and 100 ppm GA3 resulted in the highest plant height, wet weight and dry weight with values of 34.52 cm, 9.26 grams, and 6.20 g, respectively

The Growth and Yield of Soybean (Glycine max (L.) Merril var. Anjasmoro) in Peat Soil by Giving Liquid Organic Fertilizer (LOF) Banana Stem Kepok (Musa paradisiaca L.)

Soybean cultivation in peatlands in West Kalimantan has constraints, including sour soil conditions and limited nutrients available to plants. The soybean (Glycine max (L.) Merril) variety is one of the leading soybean varieties cultivated in West Kalimantan. Efforts to increase soybean production can be pursued through the application of organic fertilizers in plants. The purpose of this research was to determine the effect of giving liquid organic fertilizer (LOF) from ‘kepok’ banana (Musa paradisiaca L.) stem on the growth and yield of the soybean variety. This study used a Complete Randomized Design (CRD) with 6 treatment levels, namely negative control (without LOF), 50 mL/L, 100 mL/L, 150 mL/L, 200 mL/L, and liquid inorganic fertilizer (positive control). The results showed that the administration of LOF banana stem had a significant effect on the plant height, leaf number, branch number, root fresh weight and shoot fresh weight, root dry weight and shoot dry weight, number of effective root nodules, pod number and pod dry weight. LOF treatment of 150 mL/L gave the best effect on plant height (145.94 cm), leaf number (50.75 stalks), branch number (8.00 branches), shoot fresh weight (156.87 g), shoot dry weight (108.87 g), the effective number of root nodules (35.00 pieces), pod number (92.00 pieces) and pods dry weight (77.72 g)

PERTUMBUHAN BIBIT KRATOM MERAH (Mitragyna speciosa Korth.) DENGAN PEMBERIAN PUPUK ORGANIK CAIR KOTORAN AYAM POTONG

The aim of this research was to determine the effect of giving chicken manure liquid organic fertilizer (LOF) on the vegetative growth of kratom plants and to find out the best concentration of chicken manure LOF that can increase plant growth kratom. This study used a completely randomized design which consisted of 7 treatment levels of chicken manure concentration of LOF, namely: control(A1), 50ml/L(A2), 100ml/L(A3), 150ml/L(A4), 200ml/L(A5), 250ml/L(A6), 300ml/L (A7). The results showed that the application of LOF chicken manure had a significant effect on the number of leaves, root dry weight, and shoot dry weight, but had no significant effect on plant height, leaf area, weight root wetness, shoot wet weight and chlorophyll content. The LOF treatment of chicken manure concentration of 150ml/L was the treatment that gave the best results on kratom plant growth for the average number of leaves (16.0 strands), root dry weight (3.94 grams), and shoot dry weight (5.48 grams)

KARAKTERISASI Bacillus spp. DAN Fusarium sp. DARI TANAMAN LADA (Piper nigrum L.) DI DESA JAGA

Pepper (Piper nigrum L.) is one of the farm crop which become commodities in West Kalimantan. This research aimed to determine the morphological characteristic of Bacillus bacteria from rhizosphere of pepper and Fusarium fungi with symptoms of yellow disease. Sampling was done in pepper farm crops, Sungai Jaga Village, Bengkayang Regency. Isolation and identification were implemented at Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Tanjungpura University, Pontianak. The result of isolation and identification showed 4 bacterial isolates belong to genus Bacillus, namely Bacillus sp. BRF1, Bacillus sp. BRF2, Bacillus sp. BRF3 and Bacillus sp. BRF4 and one isolate of Fusarium sp. JDF from pepper with symptom of yellow desease

Uji Antagonis Trichoderma harzianum terhadap Erwinia sp., Penyebab Penyakit Busuk Bakteri pada Aloe vera

Aloe vera has been cultivated for many years in many parts of Indonesia, West Kalimantan Province\ud in particular. Bacterial rot disease caused by Erwinia sp. is a major limiting factor in the crop production. The\ud purpose of this study was to assess the potential use of Trichoderma harzianum as an antagonist fungus in suppressing\ud the growth of Erwinia sp., the causal agent of bacterial rot disease of A. vera. T. harzianum was isolated from soil\ud collected from A. vera plantation. The isolate was grown on PDA and then identified under a microscope. Leaves\ud showing bacterial rot symptoms were collected from the field. The bacterial pathogen, Erwinia sp. was isolated\ud from the leaf samples and grown in a growth medium for characterization. The fungal and bacterial isolates\ud were used in an antagonistic test. The results indicated that T. harzianum inhibited the growth of Erwinia sp.\ud with possible competition mechanism of space and nutrition, and mechanism of antibiosis. Competition\ud mechanism enabled T. harzianum fungi to inhibit the growth of Erwinia sp. up to 100% seven days after the\ud initiation of the test. Antibiosis test indicated that Erwinia sp. was sensitive to antibacteria which was\ud produced by T. harzianum. Therefore, the fungus could potentially be developed as biocontrol agent for the\ud bacterial disease of Aloe vera