Fate mapping melanoma persister cells through regression and into recurrent disease in adult zebrafish

Melanoma heterogeneity and plasticity underlie therapy resistance. Some tumour cells possess innate resistance, while others reprogramme during drug exposure and survive to form persister cells, a source of potential cancer cells for recurrent disease. Tracing individual melanoma cell populations through tumour regression and into recurrent disease remains largely unexplored, in part, because complex animal models are required for live imaging of cell populations over time. Here, we applied tamoxifen-inducible cre(ERt2)/loxP lineage tracing to a zebrafish model of MITF-dependent melanoma regression and recurrence to image and trace cell populations in vivo through disease stages. Using this strategy, we show that melanoma persister cells at the minimal residual disease site originate from the primary tumour. Next, we fate mapped rare MITF-independent persister cells and demonstrate that these cells directly contribute to progressive disease. Multiplex immunohistochemistry confirmed that MITF-independent persister cells give rise to Mitfa(+) cells in recurrent disease. Taken together, our work reveals a direct contribution of persister cell populations to recurrent disease, and provides a resource for lineage-tracing methodology in adult zebrafish cancer models

When a Repository Is Not Enough: Redesigning a Digital Ecosystem to Serve Scholarly Communication

INTRODUCTION Our library’s digital asset management system (DAMS) was no longer meeting digital asset management requirements or expanding scholarly communication needs. We formed a multiunit task force (TF) to (1) survey and identify existing and emerging institutional needs; (2) research available DAMS (open source and proprietary) and assess their potential fit; and (3) deploy software locally for in-depth testing and evaluation. DESCRIPTION OF PROGRAM We winnowed a field of 25 potential DAMS down to 5 for deployment and evaluation. The process included selection and identification of test collections and the creation of a multipart task based rubric based on library and campus needs assessments. Time constraints and DAMS deployment limitations prompted a move toward a new evaluation iteration: a shorter criteria-based rubric. LESSONS LEARNED We discovered that no single DAMS was “just right,” nor was any single DAMS a static product. Changing and expanding scholarly communication and digital needs could only be met by the more flexible approach offered by a multicomponent digital asset management ecosystem (DAME), described in this study. We encountered obstacles related to testing complex, rapidly evolving software available in a range of configurations and flavors (including tiers of vendor-hosted functionality) and time and capacity constraints curtailed in-depth testing. While we anticipate long-term benefits from “going further together” by including university-wide representation in the task force, there were trade-offs in distributing responsibilities and diffusing priorities. NEXT STEPS Shifts in scholarly communication at multiple levels—institutional, regional, consortial, national, and international—have already necessitated continual review and adjustment of our digital systems

A Worldwide Test of the Predictive Validity of Ideal Partner Preference-Matching

©American Psychological Association, [2024]. This paper is not the copy of record and may not exactly replicate the authoritative document published in the APA journal. The final article is available, upon publication, at: [ARTICLE DOI]”Ideal partner preferences (i.e., ratings of the desirability of attributes like attractiveness or intelligence) are the source of numerous foundational findings in the interdisciplinary literature on human mating. Recently, research on the predictive validity of ideal partner preference-matching (i.e., do people positively evaluate partners who match versus mismatch their ideals?) has become mired in several problems. First, articles exhibit discrepant analytic and reporting practices. Second, different findings emerge across laboratories worldwide, perhaps because they sample different relationship contexts and/or populations. This registered report—partnered with the Psychological Science Accelerator—uses a highly powered design (N=10,358) across 43 countries and 22 languages to estimate preference-matching effect sizes. The most rigorous tests revealed significant preference-matching effects in the whole sample and for partnered and single participants separately. The “corrected pattern metric” that collapses across 35 traits revealed a zero-order effect of β=.19 and an effect of β=.11 when included alongside a normative preference-matching metric. Specific traits in the “level metric” (interaction) tests revealed very small (average β=.04) effects. Effect sizes were similar for partnered participants who reported ideals before entering a relationship, and there was no consistent evidence that individual differences moderated any effects. Comparisons between stated and revealed preferences shed light on gender differences and similarities: For attractiveness, men’s and (especially) women’s stated preferences underestimated revealed preferences (i.e., they thought attractiveness was less important than it actually was). For earning potential, men’s stated preferences underestimated—and women’s stated preferences overestimated—revealed preferences. Implications for the literature on human mating are discussed.Unfunde

supplementary_material - Is it me or you? First-time mothers’ attributions for postpartum sexual concerns are associated with sexual and relationship satisfaction in the transition to parenthood

<p>supplementary_material for Is it me or you? First-time mothers’ attributions for postpartum sexual concerns are associated with sexual and relationship satisfaction in the transition to parenthood by Sarah A. Vannier, Kaitlyn E. Adare, and Natalie O. Rosen in Journal of Social and Personal Relationships</p

The Gulf Coast Heritage Preparedness Initiative: Proposal to the Texas A&M University President's Excellence Fund

This planning and pilot study will assess the feasibility of a community-based, interdisciplinary model for protecting endangered places and heritage in surviving historic Black settlements founded 1865-1930 known as freedom colonies. The team will develop the materials, approach, and lead model testing among freedom colony descendants in the Brazos Valley region. The team’s goal is to create a community heritage preparedness assessment protocol and process which includes public guidance, hotspot analysis, and workshop curriculum delivered through an engaged, participatory, public humanities approach. The deployment of this pilot study will determine if making existing guidance and assessments more participatory and including archival education within current engagement with freedom colonies will increase these communities’ capacity to prevent loss of culture and valuables before disaster strikes. Once tested and implementation is funded, the model will be propagated through the Gulf Coast Heritage Preparedness Initiative (GCHPI) which will leverage cross-disciplinary training and technology to enable field documentation, collection, and education throughout the State and region. A primary objective is to create sustainable, holistic approaches to community-based preparedness as the frequency and intensity of disasters increase and disproportionately impact communities of color.X-Grant, President's Excellence Fund, Texas A&M Universit

Short Rubric Scores for Texas A&M Libraries DAMS Evaluation

Microsoft Excel (xslx) and csv version of the Texas A&amp;M University Libraries DAMS Task Force Short Rubric evaluation of DSpace, Islandora, Hydra/Sufia, Nuxeo, and ResourceSpace.</p

Long Rubric Scores for Texas A&M Libraries DAMS Evaluation

MS Excel file contains 8 worksheets for each Long Rubric evaluation subsection. Each worksheet includes final evaluation scores for DSpace, Islandora, and Hydra/Sufia for that section. Sections include: Inputting and Structuring Content User Management Ticket/Request/Workflow Statistics/Reporting Discover Relational Linking Presentation External Systems A score of C denotes a feature that was not present, but could be configured. A score of T denotes a solution could not be found in the 20 minute investigation time allotted. Please see details in the associated publication. The tables in table_long_rubric_scores.xlsx and table_long_rubric_scores.csv contain summary data for the long rubric sections.</p

DI DAMS Evaluation Rubric

Analysis of 25 potential DAMS by Texas A&amp;M Univeristy Libraries IT group (Digital Initiatives). DAMS were scored on the following: Local Institutional Knowledge API Discovery Documentation Community Health Development </ul

Campus DAMS Needs Assessment

This dataset contains the DAMS Task Force Campus Needs Assessment Survey as a PDF. Survey Data is provided in MS Excel (xslx) and csv formats. Survey Results: Questions with multiple answers: answers are separated by a double pipe (||) Questions that included other and a free text area are denoted by Other: free text answer provided. </p