Efek Kualitas Kerja dalam Mengatasi Gap antara Kompetensi Kepemimpinan terhadap Kinerja

Peneliti ini bertujuan untuk mengetahui pengaruh kompetensi kepemimpinan terhadap Kualitas kerja, pengaruh kualitas kerja terhadap kinerja pegawai, dan pengaruh kompetensi kepemimpinan terhadap kinerja pegawai dengan kualitas kerja sebagai variabel mediasi. Populasi dalam penelitian ini sebesar 215 dengan sampel sebanyak 150 orang responden yang dipilih dengan metode Stratified Proportional Random Sampling model dianalisis dengan Structural Equation Modelling (SEM) dengan bantuan AMOS versi 20. Hasil analisis membuktikan bahwa kompetensi kepemimpinan berpengaruh positif dan signifikan terhadap kinerja pegawai dengan nilai koefisien sebesar 3,100, Kompetensi kepemimpinan berpengaruh positif dan signifikan terhadap kinerja pegawai, kompetensi kepemimpinan berpengaruh terhadap kualitas kerja dan kompetensi berpengaruh terhadap kinerja pagawai melalui kualitas kerja sebagai variabel mediasai. Hasil ini menunjukkan bahwa variabel kualitas kerja berperan secara signfikan sebagai variabel mediasi antara kompetensi kepemimpinan terhadap kinerja pegawai

Highly purified lipopolysaccharides from Burkholderia cepacia complex clinical isolates induce inflammatory cytokine responses via TLR4-mediated MAPK signalling pathways and activation of NFκB

In cystic fibrosis (CF), bacteria of the Burkholderia cepacia complex (Bcc) can induce a fulminant inflammation with pneumonitis and sepsis. Lipopolysaccharide (LPS) may be an important virulence factor associated with this decline but little is known about the molecular pathogenesis of Bcc LPS. In this study we have investigated the inflammatory response to highly purified LPS from different Bcc clinical isolates and the cellular signalling pathways employed. The inflammatory response (TNFα, IL-6) was measured in human MonoMac 6 monocytes and inhibition experiments were used to investigate the Toll-like receptors and associated adaptor molecules and pathways utilized. LPS from all clinical Bcc isolates induced significant pro-inflammatory cytokines and utilized TLR4 and CD14 to mediate activation of mitogen-activated protein kinase pathways, IκB-α degradation and NFκB activation. However, LPS from different clinical isolates of the same clonal strain of Burkholderia cenocepacia were found to induce a varied inflammatory response. LPS from clinical isolates of Burkholderia multivorans was found to activate the inflammatory response via MyD88-independent pathways. This study suggests that LPS alone from clinical isolates of Bcc is an important virulence factor in CF and utilizes TLR4-mediated signalling pathways to induce a significant inflammatory response. © 2006 The Authors; Journal compilation © 2006 Blackwell Publishing Ltd