Development of Dynamic Modeling and Fuzzy Logic System by Classical and Modern Strategies for the Control of Quadcopter

Quadcopters or Drones have multiple applications for different purpose such as investigation, assessment, exploration, rescue and decreasing the human strength in an adverse situation. Unmanned air vehicles (UAV) is designed with four propellers to resolve the issue of constancy however it will make the drone further more complex for the dynamic modelling and for the control system. In this research, smart controller is intended to regulate the attitude of drone. Simulation ideal model and fuzzy logic control approach is formulated to implement for four elementary motions i.e. roll, pitch, yaw, and height of our drone for the application of earth-quake. The key objective of this research paper is to develop the anticipated output as compare to the desired input

THE CREDIT SUPPLY CHANNEL OF MONETARY POLICY TRANSMISSION MECHANISM: AN EMPIRICAL INVESTIGATION OF ISLAMIC BANKS IN PAKISTAN VERSUS MALAYSIA

The transmission mechanism of monetary policy is explained through the relationships between a change in money supply and the level of real income. Monetary policy transmits to the real sector through several different channels. Such channels include the interest rate channel, the exchange rate channel, the asset-pricing channel, the credit supply channel, and the bank balance sheet channel. This paper empirically investigates the credit supply channel of monetary policy and explores the differential impact of monetary policy on credit supply of Islamic banks in Pakistan versus Malaysia. The robust two-step System-Generalize Method of Moments (GMM) estimator is applied on an unbalanced panel dataset over the period 2005-2016. While estimating the effects of three alternative measures of monetary policy on banks’ credit supply, several bank- specific variables are included in the specification as control variables. We provide strong evidence on the existence of credit supply channel in the baseline models for both countries and differential impact of monetary policy through Islamic banks in Pakistan versus Malaysia in the extended models. Our findings suggest that there is a vital need to consider the nature of Islamic banks while devising the instruments of an effective monetary policy in countries with dual banking system like Pakistan, Malaysia, Indonesia, Bahrain, Saudi Arabia, Qatar and others

Computational analysis and expression profiling of potassium transport-related gene families in mango (Mangifera indica) indicate their role in stress response and fruit development

Mango (Mangifera indica) fruit is known for its taste, health benefits, and drought tolerance. Potassium (K+) is one of the most abundant ions in a plant cell. It is important for various biological functions related to plant growth, development, and flowering/fruiting. It significantly contributes to fruit yield, quality, and drought tolerance in plants. However, molecular mechanisms comprising K+ transport in mango are least known. In the present study, 37 members of K+ transport-related genes (PTGs) were identified in mango, which include 22 K+ transporters (16 HAKs, 1 HKT, and 6 KEAs) and 15 K+ channels (6 TPKs and 8 Shakers). All PTGs were predicted to be expressed at the plasma membrane and possess characteristic motifs and domains. Phylogenetic analysis identified a strong kinship of PTGs among Oryza sativa, Arabidopsis thaliana, Cicer arietinum, Malus domestica, and M. indica. The promoter analysis identified 60 types of cis-elements related to various biological processes. RNA-seq-based expression profiling identified that MiTPK1.2, MiHAK1, MiHAK2.1, HAK6.1, and MiAKT1.1 were most upregulated in roots and that MiKEA2, MiAKT2, and MiAKT1 were upregulated in leaves. Moreover, MiAKT6, MiHAK1.1, MiKAT2, MiKAT2.1, MiHKT1, MiTPK1.1, MiHAK7, and MiHAK12 were highly expressed during the five growth stages of mango fruit. The current study is the first comprehensive report on K+ transport system in tropical fruits. Therefore, it will provide the foundation knowledge for the functional characterization of K+ genes in mango and related plants

Genome-wide association mapping of bread wheat genotypes using yield and grain morphology-related traits under different environments

Depleting water resources and increasing global temperature due to climate change are major challenges to agriculture and food security worldwide. Deciphering the underlying mechanisms of traits contributing to grain development and yield is essential for the development of climate-resilient cultivars. Therefore, this study assessed 105 bread wheat genotypes grown under control, drought, and heat-stress conditions for two crop seasons and performed a genome-wide association study (GWAS) using a 90k SNP array. The genotypes showed significant trait differences under all environmental conditions. Highly significant variation was observed, with moderate (50.09%) to high (76.19%) heritability in the studied germplasms. The studied traits were all also significantly positively correlated. A total of 541 significant associations (p ≤ 10−3) between marker and trait (MTAs) were observed after crossing the FDR <0.05 threshold for all traits. Among these, 195, 179, and 167 significant MTAs were detected under control, drought, and heat-stress conditions, respectively. Under the control and drought conditions, pleiotropic loci BS00010616_51 and BS00010868_51 were observed on chromosomes 7B and 1B situated at 186.24 cM and 35.47 cM, respectively. Pleiotropic loci BS00010868_51, Kukri_c11154_1723, and Ex_c10068_1509 were identified on chromosomes 1B, 5B, and 2A, respectively, under control and heat stress conditions. A stable and consistent locus (Excalibur_c20796_395) on chromosome 7A, located at 372.34 cM, was also linked to grain morphology and yield-related attributes in control, drought, and heat-stress conditions. The results of the current study confirmed several previously reported MTAs for the traits under consideration and identified new MTAs under harsh climatic conditions. These SNPs will aid in the discovery of novel genes in wheat. SNPs showing significant associations may be used in marker-assisted selection and allow the development of drought- and heat-tolerant genotypes with high yields to address global food security concerns

Sequence and phylogenetic analysis of virulent Newcastle disease virus isolates from Pakistan during 2009–2013 reveals circulation of new sub genotype

AbstractDespite observing the standard bio-security measures at commercial poultry farms and extensive use of Newcastle disease vaccines, a new genotype VII-f of Newcastle disease virus (NDV) got introduced in Pakistan during 2011. In this regard 300 ND outbreaks recorded so far have resulted into huge losses of approximately USD 200 million during 2011–2013. A total of 33 NDV isolates recovered during 2009–2013 throughout Pakistan were characterized biologically and phylogenetically. The phylogenetic analysis revealed a new velogenic sub genotype VII-f circulating in commercial and domestic poultry along with the earlier reported sub genotype VII-b. Partial sequencing of Fusion gene revealed two types of cleavage site motifs; lentogenic 112GRQGRL117 and velogenic 112RRQKRF117 along with some point mutations indicative of genetic diversity. We report here a new sub genotype of virulent NDV circulating in commercial and backyard poultry in Pakistan and provide evidence for the possible genetic diversity which may be causing new NDV out breaks

Suitability of various plant derived gelling agents as agar substitute in microbiological growth media

Eleven putative gelling agents were investigated as agar substitutes. These included arrowroot (Maranta arundinaceae), coconut powder (Cocos nucifera), corn flour (Zea mays var. amylacea), gel rite (a water-soluble polysaccharide produced by Sphingomonas elodea), glue (Cyanoacrylates), katira gum (Cochlospermum religiosum), guar gum (Cyamopsis tetragonolobus L.), isubgol husk (Plantago ovata), pectin and rice (Oryza sativa L.) powder. Among these, guar gum was found a promising alternate candidate for agar. Media solidified with 2.8% guar gum was transparent and supportive for the growth of three test fungi (Trichoderma harzianum, Alternaria alternata and Alternaria solani) as good as agar. Guar gum also excelled in terms of cost benefit ratio when compared with agar. Guar gum fortified media was found to cost $0.005/L as compared to agar supplemented media costing$ 1.17/L. Further, guar gum is easily available and can be added with ease thereby serving as a suitable and inexpensive substitute of agar and thus, can be adopted for routine microbiological testing in resource poor countries.Key words: Guar gum, media, agar, gelling agents

Simulation-optimization of Tarbela Reservoir operation to enhance multiple benefits and to achieve sustainable development goals

Pakistan’s agriculture and economy rely heavily on the Tarbela Reservoir. The present storage capacity of Tarbela is 8.2 BCM and it has been depleted by more than 40% due to sedimentation since 1976. It also has had a 0.94 percent (0.134 BCM) decrease in gross reservoir capacity every year. Historically, the amount of sediment trapped in the Tarbela Reservoir during the period 1976–2020 was 198.5 million tonnes annually. Based on the current operation by the Water and Power Development Authority (WAPDA), the delta is expected to extend to 2.41 km from the dam in 2035. The reservoir will become a run-of-the-river reservoir with a gross storage capacity of 2.87 BCM. This rapid loss of storage capacity will significantly impact reservoir benefits while also putting turbine performance at risk due to abrasion. Slowing the sediment deposition phenomena by a flexible operational strategy is a worthwhile aim from the dam manager’s viewpoint to achieve Sustainable Development Goals (i.e., poverty and hunger alleviation, clean affordable energy, protecting ecosystem etc.). Therefore, for the safe and long-term operation of the turbines, the existing Standard Operating Procedures (SOPs) adopted by WAPDA need to be appraised to delineate their impact on future optimized operations. The aspect of considering static SOPs on the whole period of reservoir operation has not been attempted earlier. The Tarbela Reservoir was selected as a case study to enhance the existing reservoir operation. The methodology relies upon the use of a 1-D sediment transport model in HEC-RAS to study the impact of the operational strategy on sedimentation. In conjunction, the existing reservoir operation of Tarbela was modelled in HEC-ResSim using its physical, operational, and 10-daily time-series data for simulation of releases and hydropower benefits based on a revised elevation-capacity curve for sedimentation. After calibration and validation, the model was applied to predict future reservoir operation impacts on a 5-year basis from 2025 to 2035 for determining storage capacity, irrigation releases, power production and energy generation. It was predicted that as the storage capacity of the reservoir is depleted (by application of the WAPDA current SOPs in future years), the irrigation releases would be increased in the Kharif season (April–September) by 7% and decreased by 50% in the Rabi season (October–March) with a corresponding increase in power generation by 4% and decrease by 37%, respectively, and the average annual energy generation would be decreased by 6.5%. The results showed that a gradual increase in the minimum operating level will slow down delta movement but it will reduce irrigation releases at times of high demand. The findings may assist water managers to improve the Tarbela Reservoir operation to achieve sustainable development goals and to attain societal future benefits

Effects of Iron Oxide Nanoparticle Supplementation on the Growth Performance, Serum Metabolites, Meat Quality, and Jejunal Basal Morphology in Broilers

Simple Summary: Iron is a necessary micronutrient in animal feed. Iron is an important component of hemoglobin, myoglobin, and the enzymes participating in redox reactions. The aim of the current study was to assess the effects of iron oxide nanoparticle supplementation on production performance, organ development, blood biochemistry, redox status, meat quality, and jejunal histology in broilers. The results indicated that iron oxide nanoparticle supplementation improved the feed conversion ratio, pectoral muscle, and jejunal histology. However, iron oxide nanoparticle supplementation showed no effects on visceral organ development, blood metabolites, redox status, and carcass traits. Abstract: The current research aimed to evaluate the supplemental effects of iron oxide nanoparticles (IONPs) on production performance, viscera development, blood metabolites, redox status, meat quality, and jejunal histology in broilers. A total of 300 day-old broilers were randomly divided into six groups with five replicates per group. Birds were fed on a corn soybean-based diet supplemented with 0, 20, 40, 60, or 80 mg/kg IONPs or 80 mg/kg of FeSO4 for 35 days. The feed conversion ratio (FCR) was improved in birds supplemented with 60 mg/kg IONPs. The pH24h was lower in birds supplemented with 40 mg/kg IONPs compared to that of the bulk group. Pectoral muscle fascicle diameter and fiber density were significantly increased in 20 mg/kg IONP-supplemented birds compared to those of the bulk group, respectively. The muscle fiber diameter was higher in 40 mg/kg IONP-supplemented birds compared with the bulk group. The jejunal villus height, crypt depth, and villus surface area were significantly increased with 60 mg/kg IONP supplementation, whereas villus width was decreased in birds supplemented with 40 mg/kg IONPs. The villus-height-to-crypt-depth ratio was lower in IONP-supplemented birds compared to the bulk group. IONP supplementation improved the FCR, jejunal, and pectoral muscle morphology without affecting the carcass characteristics and redox status of broilers

Collection and Evaluation of Genetic Diversity and Population Structure of Potato Landraces and Varieties in China

China is the world’s leading country for potato production but potato is not native to China. To gain insights into the genetic diversity of potato germplasm various studies have been performed but no study has been reported for potato landraces in China. To improve the available genepool for future potato breeding programs, a diverse population containing 292 genotypes (including foreign elite lines, local landraces and cultivars) was developed and genotyped using 30 SSR markers covering the entire potato genome. A total of 174 alleles were detected with an average of 5.5 alleles per locus. The model-based structure analysis discriminated the population into two main sub-groups, which can be further subdivided into seven groups based on collection sites. One sub-group (P1) revealed less genetic diversity than other (P2) and contained a higher number of commercial cultivars possibly indicating a slight reduction in diversity due to selection in breeding programs. The P2 sub-group showed a wider range of genetic diversity with more new and unique alleles attained from wild relatives. The potato landraces, clustered in sub-population P1 may be derived from historical population imported from ancient European and International Potato Center genotypes while sub-population P2 may be derived from modern populations from International Potato Center and European genotypes. It is proposed that in the first step, the potato genotypes were introduced from Europe to China, domesticated as landraces, and then hybridized for modern cultivars

A whole genome sequencing approach to anterior cruciate ligament rupture–a twin study in two unrelated families

Predisposition to anterior cruciate ligament (ACL) rupture is multi-factorial, with variation in the genome considered a key intrinsic risk factor. Most implicated loci have been identified from candidate gene-based approach using case-control association settings. Here, we leverage a hypothesis-free whole genome sequencing in two two unrelated families (Family A and B) each with twins with a history of recurrent ACL ruptures acquired playing rugby as their primary sport, aimed to elucidate biologically relevant function-altering variants and genetic modifiers in ACL rupture. Family A monozygotic twin males (Twin 1 and Twin 2) both sustained two unilateral non-contact ACL ruptures of the right limb while playing club level touch rugby. Their male sibling sustained a bilateral non-contact ACL rupture while playing rugby union was also recruited. The father had sustained a unilateral non-contact ACL rupture on the right limb while playing professional amateur level football and mother who had participated in dancing for over 10 years at a social level, with no previous ligament or tendon injuries were both recruited. Family B monozygotic twin males (Twin 3 and Twin 4) were recruited with Twin 3 who had sustained a unilateral non-contact ACL rupture of the right limb and Twin 4 sustained three non-contact ACL ruptures (two in right limb and one in left limb), both while playing provincial level rugby union. Their female sibling participated in karate and swimming activities; and mother in hockey (4 years) horse riding (15 years) and swimming, had both reported no previous history of ligament or tendon injury. Variants with potential deleterious, loss-of-function and pathogenic effects were prioritised. Identity by descent, molecular dynamic simulation and functional partner analyses were conducted. We identified, in all nine affected individuals, including twin sets, non-synonymous SNPs in three genes: COL12A1 and CATSPER2, and KCNJ12 that are commonly enriched for deleterious, loss-of-function mutations, and their dysfunctions are known to be involved in the development of chronic pain, and represent key therapeutic targets. Notably, using Identity By Decent (IBD) analyses a long shared identical sequence interval which included the LINC01250 gene, around the telomeric region of chromosome 2p25.3, was common between affected twins in both families, and an affected brother’. Overall gene sets were enriched in pathways relevant to ACL pathophysiology, including complement/coagulation cascades (p = 3.0e-7), purine metabolism (p = 6.0e-7) and mismatch repair (p = 6.9e-5) pathways. Highlighted, is that this study fills an important gap in knowledge by using a WGS approach, focusing on potential deleterious variants in two unrelated families with a historical record of ACL rupture; and providing new insights into the pathophysiology of ACL, by identifying gene sets that contribute to variability in ACL risk