Therapeutic effects of avocado (Persea americana Mill.) seed powder against reproductive toxicity induced by Mancozeb (herbicide) in female rabbits

DATA AVAILABILITY STATEMENT : The authors declare no competing interests.Pesticides like Mancozeb are being increasingly indispensable in the control of crop pests. Unfortunately, they have been implicated in genotoxicity due to their ubiquity, toxicological properties, persistence and presence in the food chain. This study sought to evaluate the efficacy of powdered avocado seed on reproductive parameters in the management of oxidative stress in female rabbits caused by the herbicide Mancozeb. Twenty-eight female rabbits aged 7–8 months and weighing between 2780.4 g and 3143.7 g were randomly divided into four groups of seven rabbits each. Each group received for 90 consecutive days distilled water or Mancozeb associated or not with avocado seed powder orally as follows: T1: 10 ml distilled water; T2, T3 and T4: 100 mg/kg bw Mancozeb. This was followed by oral administration of 250, 500, and 0 mg/kg of avocado seed powder for T2, T3, and T4, respectively. Water and feed were distributed ad libitum. Collected data concerned growth, carcass and reproductive performances, hematological and biochemistry characteristics. Results demonstrated that pregnant and lactating female rabbits administered Mancozeb exhibited a significant decrease (P < 0.05) in food intake, body weight, and body weight gain. Female rabbits exposed to Mancozeb had a decrease in litter size and weight from birth to weaning, as well as in weaning body weight and weight increase, fertility and prolificacy rate, milk yield, and daily milk efficiency. However, administration of avocado seed powder reversed (P < 0.05) the trends in these parameters in a dose-dependent manner. The increase in relative weight of the kidney and liver, concentrations of urea, creatinine, alanine and aspartate aminotransferases, mean cell volume, white blood cells, and lymphocytes were all associated with increased Mancozeb rates (P < 0.05). On the contrary, administration of the Mancozeb caused decrease in hemoglobin (Hb), Red blood Cell (RBC) and protein content. Administration of avocado seed powder significantly (P < 0.05) ameliorated the Mancozeb effects on these parameters. Applying 500 mg/kg b.w Avocado seed powder may be suggested as an alternative therapy for reproductive defects induced by Mancozeb in female rabbits.https://www.cell.com/heliyon/homeZoology and Entomolog

Typology of smallholder’s pig production systems in South Kivu, Democratic Republic of Congo: Challenges and opportunities

Pig farming plays an important role in farmers’ livelihoods in many tropical countries. It contributes to food security of the poorest as well as the development of rural economy through multiplier effects. In the South Kivu province, pig farms are almost exclusively owned by smallholders. A few studies have attempted to describe thoroughly pig farming systems in this province. This study was undertaken to characterise pig production systems, in order to better understand their current situation, namely constraints they face and opportunities they offer. Investigation was conducted based on a structured survey questionnaire and participatory interviews with the owners of 989 farms in South-Kivu. Collected data was analysed using Multiple Correspondence Analysis and clustering techniques. Results showed that there are two types of smallholder pig farms differing mainly in the type of husbandry and feeding management. One category includes farms that raise pigs in free-range system consuming forages and scavenge feed (heaps picked-up from garbage and trash on their ways), which are sometimes, combined with crop residues and kitchen leftovers. The second category includes improved pig farms raising tethered pigs or in lairage where feed is mostly based on forages combined with kitchen leftovers, crop residues and concentrate feed. Nevertheless, all these different farm types share many common characteristics, including having pigs of local breeds, small herd sizes, absence of breeding boars and absence of adequate prophylactic measures. It emerged that female farmers together with experienced farmers mainly own pig farms with better characteristics (breed type, management practices, litter size, etc.). Hence, the involvement of women in pig farming can offer better prospects for the improvement of this sector. In addition, access to agricultural credit can also be an alternative to foster investment in livestock in South Kivu. All this can only lead to better results though improved local market access to smallholder producers

Neglected and underutilized crop species in Kabare and Walungu territories, Eastern D.R. Congo: Identification, uses and socio-economic importance

peer reviewedNeglected and underutilized crop species (NUCS) are valuable sources of food and income for several rural households in the eastern Democratic Republic of Congo (DRC). A thorough understanding of their diversity, uses, socioeconomic roles, and factors for their neglect would be necessary for guiding promotion efforts. This study was conducted in two steps: (1) interviews with rural households involved in NUCS production and trade, and (2) greenhouse characterization of the two most popular NUCS ′ morphotypes, African nightshade and amaranth, from Walungu and Kabare territories, eastern DRC. About 19% of farmers of the entire study area were involved in NUCS production. We identified 22 and 23 NUCS used in Kabare and Walungu territories, respectively. These species belonged to 19 botanical families, among which Solanaceae (~24% in Walungu and ~11 in Kabare) and Fabaceae (8% in Walungu and 11% in Kabare) were the most dominant families. They were of different ranges: grasses and herbs (62%), shrubs (20%), thorns (2%), and trees (16%). These species were used whether for nutritional (60%), medicinal (7%), or both nutritional and medicinal values (33%). NUCS' market value was low, and thus, discouraged further investment in their farming. The seed delivery system was informal, dominated by farmer-saved seed, seed from local markets, and farmer seed exchange. Characterization experiment for the most popular leafy NUCS showed differences among collected morphotypes for most traits, with some local genotypes outperforming exogenous commercial varieties. Supply of quality seed, training of farmers on the most appropriate cropping system, processing technologies, increased consumer awareness, linking research to policy, and increasing market opportunities for smallholder farmers are strategies for promoting wide use of these crops in eastern DRC

Gully erosion susceptibility mapping using four machine learning methods in Luzinzi watershed, eastern Democratic Republic of Congo

peer reviewedSoil erosion by gullying causes severe soil degradation, which in turn leads to severe socio-economic and environmental damages in tropical and subtropical regions. To mitigate these negative effects and guarantee sustainable management of natural resources, gullies must be prevented. Gully management strategies start by devising adequate assessment tools and identification of driving factors and control measures. To achieve this, machine learning methods are essential tools to assist in the identification of driving factors to implement site-specific control measures. This study aimed at assessing the effectiveness of four machine learning methods (Random Forest (RF), Maximum of Entropy (MaxEnt), Artificial Neural Network (ANN), and Boosted Regression Tree (BRT)) to identify gully's driving factors, and predict gully erosion susceptibility in the Luzinzi watershed, in eastern Democratic Republic of Congo (DRC)

Assessing milk products quality, safety, and influencing factors along the dairy value chain in eastern Democratic Republic of the Congo

Dairying is one of the new promising economic sectors in eastern Democratic Republic of Congo (DRC), but still not explored enough to ensure consumers' safety. This study aimed to assess the health risks and nutritional profile of milk products along the value chain in South-Kivu and Tanganyika provinces. A total of 288 milk actors, including 160 producers, 35 collectors and 93 vendors, were concerned for interview and milk samples collection. A total of 302 milk samples (159 raw, 44 pasteurized, 76 fermented and 19 white cheese so-called “Mashanza”) were collected for physicochemical [pH, fat, non-fat dry matter (NFDM), lactose, protein, freezing point, density] and microbiological (total Aerobic Mesophilic Flora, Escherichia coli, Total Coliforms, Fecal Coliforms, Salmonella and Staphylococci) analyses. Results revealed that the physicochemical characteristics of the milk mostly varied according to the type of milk and the regions. The pasteurized milk from Tanganyika presented the best physicochemical parameters [crude protein (CP) = 4.36%, Fat = 4.06%, NFDM = 12%, lactose = 5.4%, density = 1.02 and pH = 6.59] compared to other types of milk. For microbiology, no E. coli was recorded but Salmonella and Staphylococci were found in all the milk types with the values not exceeding 3 × 104 CFU ml−1 and 3 × 103 CFU ml−1, respectively. This implies a long-term consumers' health issue if appropriate measures are not taken by milk actors along the value chain. The microbiological quality was influenced by the ecologies of production axis (representing the production zones) and by handling methods and infrastructures used by the actors involved along the value chain. Factors related to animal husbandry, milking method, milk processing and packaging had no significant effect on the physicochemical parameters under study. These results indicated that health risks for milk consumers are accrued by production practices and handling by milk actors due to shortage of required skills and appropriate equipment along the milk value chain. Observance of hazard analysis critical control point (HACCP) measures is carefully required along the milk value chain nodes to improve the quality of milk produced and sold and thus reduce the risks among consumers in South-Kivu and Tanganyika provinces

Suitability for agroforestry implementation around Itombwe Natural Reserve (RNI), eastern DR Congo: Application of the Analytical Hierarchy Process (AHP) approach in geographic information system tool

Different land use suitability assessment approaches exist and have been applied for agroforestry systems, natural or semi-natural ecosystems. In this paper, different land use suitability levels for agroforestry were assessed around the Itombwe Natural Reserve (DRCongo). Information related to soil unity, topography, climate, road infrastructures, rivers, villages, land use and land cover were analyzed using the Analytical Hierarchy Process (AHP). Moreover, spatial analyses were conducted for determining the matrix that confronts adopted variables and their corresponding weights. Results allowed identifying four agroforestry suitability zones, ranging from very high, high, moderate and low suitable zones

Analyse des changements spatio-temporels des occupations des sols dans les territoires de Kabare et Walungu au Sud-Kivu entre 2000 et 2018 : Utilisation des outils de la télédétection et du modèle Random Forest

L’évolution des occupations des sols est l’une des transformations les plus immenses et les plus perceptibles de la surface de la terre. L’évaluation de ces changements des occupations des sols est impérative dans un large éventail de perspectives telles que la conservation de l’environnement et la gestion des ressources naturelles dans une perspective de durabilité. Ce travail examine les changements des occupations des sols dans les territoires de Kabare et Walungu entre 2000 et 2018 en utilisant des images satellite Landsat et le modèle Random Forest qui s’est montré performant en donnant une bonne précision. Il s’observe qu’il y a eu des changements substantiels dans les occupations des sols durant la période considérée. Les changements les plus remarquables s’observent au sein des mosaïques champs-jachères, les savanes herbeuses, les plantations sylvicoles les forêts denses et les forêts secondaires. Les changements qu’il y a eu sont essentiellement des dégradations. Des fortes augmentations des superficies des mosaïques champs-jachères (320 km²) et savanes herbeuses (202 km²) se sont observées. Par contre, il y a eu des fortes diminutions des superficies des forêts denses (178 km²), les forêts secondaires (141 km²) et les plantations sylvicoles (195 km²). Les forêts denses ont été dégradées en forêts secondaires ; Les forêts secondaires telles qu’elles étaient en 2000, bien qu’une partie ait évolué en forêts denses, la majeure partie a essentiellement été dégradée en mosaïques champs-jachères ou plantations sylvicoles. Les mosaïques champs-jachères qu’on retrouve en 2018 sont issues de celles des années 2000, des anciennes plantations sylvicoles, savanes herbeuses ou de la dégradation des forêts secondaires

Aflatoxin and fumonisin mycotoxins contamination along the maize value chain in the eastern Democratic Republic of Congo

Aflatoxin and fumonisin contamination was assessed in different samples along the maize value chain in different territories of South Kivu province. Kabare and Ruzizi Plain were chosen as they represent two different agroecological areas where maize is mostly produced. Twelve districts and one town were selected across the province. The stakeholders were randomly selected, and 215 maize (139 maize grain and 76 maize flour) samples were taken for laboratory analysis. The Q + kit was used to determine the total aflatoxins and fumonisins. Three categories of maize were examined: freshly harvested dry maize, stored maize (maize stored for 3 months ±1.5 month) and market maize. Aflatoxin was found in 100% of the maize samples with the least content of 0.3 µg/kg detected in freshly harvested dry maize with mean 3.2+0.3 and levels ranging from 0.3 to 18.5 µg/kg. The average level of aflatoxin in stored grain samples was 97.9±182 µg/kg within a range of 1.16 to 841.5 µg/kg, and the mean level of aflatoxin in stored flour was 148.9±164.5 µg/kg with levels ranging from 2.05 to 905.1 µg/kg. The mean level of aflatoxin maize collected from the market was 95.1 ±164 µg/kg, with levels ranging from 1 to 823.2 µg/kg. Almost all the maize flour collected from the three areas had a high contamination level that exceeded the maximum tolerable limit of 10 µg/kg. Fumonisin was detected in all samples. However, the levels of fumonisin do not follow a specific trend with the duration of storage. The freshly harvested dry maize concentration was 2.4±5.1 µg/g, with levels ranging from 0.03 to 20.9µg/g. About 37% of freshly harvested maize samples contaminated by fumonisin exceeded the maximum tolerable limit of 4 µg/kg. There was a difference between total fumonisin in grain and flour; the average level of fumonisin in stored maize grain was 1.4±0.9 µg/g with levels ranging from 0.18- 4.7 µg/g while in flour, the level was 2.1±1.3 µg/g with levels ranging from 0.3-4.5 µg/g. Almost all the maize samples collected from the three areas had a degree of contamination that did not exceed the maximum tolerable limit of 4 µg/g. These results indicate that the two mycotoxin levels, particularly aflatoxin, were high in the different samples collected at specific nodes. Therefore, preventing mycotoxins accumulation in maize by post-harvest prevention of contamination and growth of toxigenic moulds by promoting proper grain drying and storage should be encouraged among the actors of the maize value chain

Characterization of smallholder cattle production systems in South-Kivu province, eastern Democratic Republic of Congo

In South-Kivu province, cattle farming is an integral component of farmers’ livelihoods and one of the few income-generating opportunities for smallholders. However, very few studies have been conducted to characterize smallholders’ cattle production systems. This study documents cattle production systems to better understand their current situation, constraints they face and opportunities they offer. For that purpose, an investigation was conducted based on a structured survey questionnaire and participatory interviews with 863 farmers in South-Kivu province. Collected data were analysed using factorial analysis of mixed data and clustering techniques. The results revealed three types of smallholder cattle farms differing mainly in their herds’ sizes and landholding. The first category is the most common and includes farmers raising small herds (6.3 ± 6.7 cattle) of local breeds in herding system (in this work, “herding system” refers to a rearing system for which the farmer drives and stays with his animals on pastures and fallow land during the day) and grazing fodder in community pastures, fallow lands and roadside grasses, while land constitutes a scarce resource. In the second category, some farmers have small tracts of land ( 5 ha), but all have medium-size herds (45.1 ± 19.4 cattle) made up of local breeds, which they rear in herding system. They also exploit community pastures, fallow land and roadside fodder for animal feeding. The third and last category includes farmers with large cattle herds (78.1 ± 28.1 cattle) of local, crossbred and exotic breeds raised free range in the fenced paddocks on vast areas of land (> 5 ha) found in high-altitude regions. However, while being different according to the above-considered characteristics, the three categories of cattle farming remain extensive pastoral farms dominated by male farmers. Agriculture and/or animal husbandry are their main source of income while their livestock are also composed of goats and poultry, beside cattle. Still, the three farming groups require more inputs and improvement strategies for increased productivity in the challenging environment characterized by low land accessibility and high demand for milk and meat. Fodder cultivation and crop-livestock integration through agro-ecological systems as well as access to credit and extension services are the proposed strategies for the improvement of this economic sector