Sesame harvest loss caused by sesame seed bug, Elasmolomus sordidus F. at Kafta-Humera sesame fields

Sesame is an important cash crop in Ethiopia. In Humera, farmers are largely dependent on this important cash crop for their living. The production rate is, however, lower than the national average. There could be many factors for its reduced productivity, but pests especially sesame seed bug is one of the reasons. In this study, sesame seed weight loss due to sesame seed bug was determined at field and laboratory conditions. At both conditions, there was significant seed weight loss when the packaging materials were unlined jute and unlined polythene bags, although the loss at field conditions was severe. In both setups, there was small seed weight loss when the packaging materials were lined. This could be due to moisture loss from the seeds because the bugs could not penetrate the lined bags. From these, farmers are advised to use lined bags for storage and transport the harvested seeds to their stores as soon as possible

Studies on the Biology and Eco Physiology of Sesame Seed Bug, Elasmolomus Sordidus Fabricius, at Kafta - Humera Sesame Fields

The experiment was conducted in the plant protection laboratory of the Humera Agricultural Research Center to study the biology of sesame seed bug, Elasmolomus sordidus (Fabricius), Hemiptera: Lygaeidae. E.sordidus is considered as the most important harvest and post-harvest insect pest of sesame in North western Ethiopia. The rearing and other tests were conducted in the laboratory at 28-330C and 50 to 65% RH. The result of this laboratory investigation indicated that the mean egg incubation period was 5.4± 0.05 days while the total mean nymphal duration took 18.44±0.10. A mean adult lifespan of 20.24±0.19 days was recorded. The mean lifespan from egg to adult death was 44.08 days, which range from 32-54 days. The reproductive period was 7.08 ± 0.26 days and the peak laying period was on the first and second day of its reproductive days. Even though E.sordidus was present the whole year high outbreak was recorded at harvest (October) and continued up to January, which afterward declined until the next harvest time. &nbsp

Examining Chlorophyll Extraction Methods in Sesame Genotypes: Uncovering Leaf Coloration Effects and Anatomy Variations

This study focuses on optimizing chlorophyll extraction techniques, in which leaf discs are cut from places on the leaf blade to enhance chlorophyll concentration in sesame (Sesamum indicum L.) leaves. Thirty sesame genotypes, categorized into light green (LG), middle green (MG), and deep green (DG) pigment groups based on leaf coloration, were selected from a larger pool of field-grown accessions. The investigation involved determining optimal Soil Plant Analysis Development (SPAD) value index measurements, quantifying pigment concentrations, exploring extraction solvents, and selecting suitable leaf disk positions. Significant variations in chlorophyll content were observed across genotypes, greenness categories, and leaf disk positions. The categorization of genotypes into DG, MG, and LG groups revealed a correlation between leaf appearance and chlorophyll content. The study highlighted a consistent relationship between carotenoids and chlorophyll, indicating their role in adaptation to warm environments. An examination of leaf disk positions revealed a significant chlorophyll gradient along the leaf blade, emphasizing the need for standardized protocols. Chlorophyll extraction experiments identified DMSO and 96% ethanol, particularly in those incubated for 10 min at 85 °C, as effective choices. This recommendation considers factors like cost-effectiveness, time efficiency, safety, and environmental regulations, ensuring consistent and simplified extraction processes. For higher chlorophyll extraction, focusing on leaf tips and the 75% localization along the sesame leaf blade is suggested, as this consistently yields increased chlorophyll content. Furthermore, our examination revealed significant anatomical variations in the internal structure of the mesophyll tissue leaves between deep green and light green sesame plants, primarily linked to chloroplast density and pigment-producing structures. Our findings, therefore, provide insightful knowledge of chlorophyll gradients and encourage the use of standardized protocols that enable researchers to refine their experimental designs for precise and comparable chlorophyll measurements. The recommended solvent choices ensure reliable outcomes in plant physiology, ecology, and environmental studies

Post-Harvest Insect Pests and Their Management Practices for Major Food and Export Crops in East Africa: An Ethiopian Case Study

Ethiopian subsistence farmers traditionally store their grain harvests, leaving them open to storage pests and fungi that can cause contamination of major staple crops. Applying the most effective strategy requires a precise understanding of the insect species, infestation rates, storage losses, and storage conditions in the various types of farmers’ grain stores. This study did a complete literature analysis on post-harvest pest and management measures with a focus on Ethiopia. The most frequent insect pests of stored cereals in this study were weevils (Sitophilus spp.), the lesser grain borer (Rhyzopertha dominica), rust-red flour beetle (Tribolium sp.), sawtoothed grain beetle (Oryzaephilus sp.), grain beetle (Cryptolestes spp.), Indian meal moth (Plodia interpunctella), and Angoumois grain moth (Sitotroga cerealella). Flour beetles (Tribolium spp.), sawtoothed beetles (Oryzaephilus sp.), flat grain beetles (Cryptolestes pusillus), and some moths have been identified as common stored product pests of stored oil seed, while bruchid beetles (Callosobruchus chinensis) and the moths were reported for pulses. Additionally, the storage pests in Ethiopia under varied conditions caused storage losses of 9–64.5%, 13–95%, 36.9–51.9%, and 2–94.7% in maize, sorghum, chickpeas, and sesame, respectively. To reduce the losses incurred, preventative measures can be taken before infestations or as soon as infestations are discovered. A variety of pest population monitoring systems for harvested products and retailers have been developed and recommended. In this context, reducing post-harvest grain losses is an urgent concern for improving food accessibility and availability for many smallholder farmers in Ethiopia and ensuring the nation’s long-term food security

Resequencing of 410 Sesame Accessions Identifies SINST1 as the Major Underlying Gene for Lignans Variation

Sesame is a promising oilseed crop that produces specific lignans of clinical importance. Hence, a molecular description of the regulatory mechanisms of lignan biosynthesis is essential for crop improvement. Here, we resequence 410 sesame accessions and identify 5.38 and 1.16 million SNPs (single nucleotide polymorphisms) and InDels, respectively. Population genomic analyses reveal that sesame has evolved a geographic pattern categorized into northern (NC), middle (MC), and southern (SC) groups, with potential origin in the southern region and subsequent introduction to the other regions. Selective sweeps analysis uncovers 120 and 75 significant selected genomic regions in MC and NC groups, respectively. By screening these genomic regions, we unveiled 184 common genes positively selected in these subpopulations for exploitation in sesame improvement. Genome-wide association study identifies 17 and 72 SNP loci for sesamin and sesamolin variation, respectively, and 11 candidate causative genes. The major pleiotropic SNPC/A locus for lignans variation is located in the exon of the gene SiNST1. Further analyses revealed that this locus was positively selected in higher lignan content sesame accessions, and the “C” allele is favorable for a higher accumulation of lignans. Overexpression of SiNST1C in sesame hairy roots significantly up-regulated the expression of SiMYB58, SiMYB209, SiMYB134, SiMYB276, and most of the monolignol biosynthetic genes. Consequently, the lignans content was significantly increased, and the lignin content was slightly increased. Our findings provide insights into lignans and lignin regulation in sesame and will facilitate molecular breeding of elite varieties and marker-traits association studies

Characteristics of deceased women of reproductive age in Tigray, Northern Ethiopia, 2020–2022 (n = 832).

Characteristics of deceased women of reproductive age in Tigray, Northern Ethiopia, 2020–2022 (n = 832).</p

Cause-specific mortality fraction (CSMF) among women of reproductive age death in Tigray, Northern Ethiopia, 2020–2022 (n = 832).

A) Overall CSMF, B) CSMF of communicable and maternal diseases group, C) CSMF of non-communicable diseases group, D) CSMF of external causes or injuries. Note that the survey weighting we used may not directly impact the assignment of causes to individual deaths. This applies specifically when using the verbal autopsy tool.</p

Group-based Cause-Specific Mortality Fraction (CSMF) versus age group among women of reproductive age death in Tigray, Northern Ethiopia, 2020–2022 (n = 832).

Group I: Communicable, maternal, and nutritional disorders, Group II: Non-communicable diseases, and Group III: External causes (Injuries). Note that the survey weighting we used may not directly impact the assignment of causes to individual deaths. This applies specifically when using the verbal autopsy tool.</p

List of all causes of death among women of reproductive age death in Tigray, Northern Ethiopia, 2020–2022 (n = 832).

List of all causes of death among women of reproductive age death in Tigray, Northern Ethiopia, 2020–2022 (n = 832).</p

S1 Dataset -

BackgroundIn resource-limited countries with weak healthcare systems, women of reproductive age are particularly vulnerable during times of conflict. In Tigray, Ethiopia, where a war broke out on 04 November 2020, there is a lack of information on causes of death (CoD) among women of reproductive age. This study aims to determine the underlying CoD among women of reproductive age during the armed conflict in Tigray.MethodsThis community-based survey was carried out in six Tigray zones, excluding the western zone for security reasons. We used a multistage stratified cluster sampling method to select the smallest administrative unit known as Tabiya. Data were collected using a standardized 2022 WHO Verbal Autopsy (VA) tool. The collected data were analyzed using the InterVA model using R analytic software. The study reported both group-based and cause-specific mortality fractions.ResultsA total of 189,087 households were screened and 832 deaths were identified among women of reproductive age. The Global Burden of Disease classification showed that infectious and maternal disorders were the leading CoD, accounting for 42.9% of all deaths. External causes contributed to 26.4% of fatalities, where assault accounted for 13.2% of the deaths. Maternal deaths made up 30.0% of the overall mortality rate. HIV/AIDS was the primary CoD, responsible for 13.2% of all deaths and 54.0% of infectious causes. Other significant causes included obstetric hemorrhage (11.7%) and other and unspecified cardiac disease (6.6%).ConclusionsThe high proportion of infectious diseases related CoD, including HIV/AIDS, as well as the occurrence of uncommon external CoD among women, such as assault, and a high proportion of maternal deaths are likely the result of the impact of war in the region. This highlights the urgent need for targeted interventions to address these issues and prioritize sexual and reproductive health as well as maternal health in Tigray.</div