An Address Delivered at the Inauguration of Rev. Joseph Muenscher and Rev. John R. Cotting as Instructors in the Female Classical Seminary, Brookfield

Includes the speech delivered by Joseph Muenscher, names of members of the Board of Visitors, Trustees, instructors, course of study and textbooks used at the Female Classical Seminary

De Taciti Germania

https://repository.brynmawr.edu/digitizedbooks/1099/thumbnail.jp

Leukoencephalopathy upon disruption of the chloride channel ClC-2

ClC-2 is a broadly expressed plasma membrane chloride channel that is modulated by voltage, cell swelling, and pH. A human mutation leading to a heterozygous loss of ClC-2 has previously been reported to be associated with epilepsy, whereas the disruption of Clcn2 in mice led to testicular and retinal degeneration. We now show that the white matter of the brain and spinal cord of ClC-2 knock-out mice developed widespread vacuolation that progressed with age. Fluid-filled spaces appeared between myelin sheaths of the central but not the peripheral nervous system. Neuronal morphology, in contrast, seemed normal. Except for the previously reported blindness, neurological deficits were mild and included a decreased conduction velocity in neurons of the central auditory pathway. The heterozygous loss of ClC-2 had no detectable functional or morphological consequences. Neither heterozygous nor homozygous ClC-2 knock-out mice had lowered seizure thresholds. Sequencing of a large collection of human DNA and electrophysiological analysis showed that several ClC-2 sequence abnormalities previously found in patients with epilepsy most likely represent innocuous polymorphisms

Reversibility of the effects of GnRH-vaccination used to suppress reproductive function in mares

Reasons for performing study: Active immunisation against gonadotrophin-releasing hormone (GnRH) provides a reversible method for control of oestrous behaviour and fertility in mares. Previous reports failed to demonstrate the interval to resumption of cyclic ovarian activity after GnRH-vaccination. Hypothesis: Administration of the GnRH-vaccine ImprovacÒ1 in a large group of mares of various ages will result in effective, reliably reversible suppression of ovarian activity within a two-year period. Methods: The mares, subdivided into three age categories were vaccinated twice (with a 35 d interval) using ImprovacÒ1 and were monitored via blood samples until Day 720 after initial vaccination for serum progesterone concentration determination by radio-immune assay and anti-GnRH antibody titre by enzyme immuno-assay. Samples were collected until individuals resumed cyclic ovarian activity. Results: All mares showed suppression of cyclic ovarian activity (SPC <1 nmol/l) and 92.2% resumed cyclic activity at Day 720 with a mean interval = 417.8 d (SD = 23.19) and median = 344 d. A significant age effect (P=0.028) on the interval, but not on GnRH-AB titre response, was observed between the youngest (11 years) categories. Conclusions: Immunising adult mares of all ages with ImprovacÒ1 resulted in a reversible suppression of cyclic ovarian activity in most mares. An age effect, with the youngest mares showing a longer interval to reversibility was observed.The Equine Research Centre of the University of Pretoriahttp://www.evj.co.uk/journals/hb2013ab201

Validation of noninvasive monitoring of adrenocortical endocrine activity in ground-feeding aardwolves (Proteles cristata) : exemplifying the influence of consumption of inorganic material for fecal steroid analysis

Biologically inert material in feces may confound interpretations of noninvasive fecal endocrine data, because it may induce variance related to differences in foraging behavior rather than to differences in endocrine activity. We evaluated two different enzyme immunoassays (EIAs) for the noninvasive evaluation of adrenocortical activity in ground-feeding aardwolves (Proteles cristata) and tested the influence of soil content in aardwolf feces on the interpretation of fecal glucocorticoid metabolite data. Using adrenocorticotropic hormone (ACTH) challenges for validation, we successfully identified a cortisol EIA suitable for assessing adrenocortical activity in aardwolves. An alternatively tested 11-oxoetiocholanolone EIA failed to detect a biologically relevant signal after ACTH administration. Although the proportion of inorganic content in aardwolf feces did not alter qualitative conclusions from the endocrine data, the data related to mass of organic content had a larger amount of variance attributed to relevant biological contrasts and a lower amount of variance attributed to individual variation, compared with data related to total dry mass of extracted material. Compared with data expressed as dry mass of extracted material, data expressed as mass of organic content may provide a more refined and statistically powerful measure of endocrine activity in species that ingest large amounts of indigestible material.A National Research Foundation (NRF) South African Research Chair to N.C.B., an NRF focal area grant to E.Z.C., and two research fellowships from the University of Pretoria to F.D. and A.G.http://www.journals.uchicago.edu/toc/pbz/currentab201

Spartina patens and other saline plants in the Genesee Valley of western New York

Volume: 29Start Page: 138End Page: 13

ADDITIONS TO OUR KNOWLEDGE OF THE FLORA OF MOUNT BAKER, WASHINGTON. II

Volume: 5Start Page: 236End Page: 23