Detrital-zircon geochronology and provenance of the Ocloyic synorogenic clastic wedge, and Ordovician accretion of the Argentine Precordillera terrane

The Precordillera terrane in northwestern Argentina is interpreted to be anexotic (Laurentian) continental fragment that was accreted to western Gondwanaduring the Ordovician. One prominent manifestation of the subductionand collision process is a Middle?Upper Ordovician clastic wedge, which overliesa passive-margin carbonate-platform succession in the Precordillera. U/Pbages of detrital zircons from sandstones within the clastic wedge, as well as zirconsfrom clasts within conglomerates, provide documentation for the compositionof the sediment provenance. The ages of detrital zircons are consistentvertically through the succession, as well as laterally along and across strike ofthe Precordillera, indicating a single, persistent sediment source throughoutdeposition of the clastic wedge. The dominant mode (~1350?1000 Ma) of thedetrital-zircon ages corresponds to the ages of basement rocks in the WesternSierras Pampeanas along the eastern side of the Precordillera. A secondarymode (1500?1350 Ma) corresponds in age to the Granite-Rhyolite province ofLaurentia, an age range which is not known in ages of basement rocks of theWestern Sierras Pampeanas; however, detritus from Granite-Rhyolite-age rocksin the basement of the Precordillera was available through recycling of synriftand passive-margin cover strata. Igneous clasts in the conglomerates haveages (647?614 Ma) that correspond to the ages of minor synrift igneous rocks inthe nearby basement massifs; the same ages are represented in a minor mode(~750?570 Ma) of detrital-zircon ages. A quartzite clast in a conglomerate, aswell as parts of the population of detrital zircons, indicates the importanceof a source in the metasedimentary cover of the leading edge of the Precordillera.The Famatina continental-margin magmatic arc reflects pre-collisionsubduction of Precordillera lithosphere beneath the western Gondwana margin;however, no detrital zircons have ages that correspond to Famatina arcmagmatism, indicating that sedimentary detritus from the arc may have beentrapped in a forearc basin and did not reach the foreland. The indicators ofsedimentary provenance for the foreland deposits are consistent with subductionof the Precordillera beneath western Gondwana, imbrication of basementrocks from either the Precordillera or Gondwana into an accretionary complex,and recycling of deformed Precordillera cover rocks.Fil: Thomas, William A.. Geological Survey of Alabama; Estados UnidosFil: Astini, Ricardo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; ArgentinaFil: Mueller, Paul A.. Florida State University; Estados UnidosFil: McClelland, William C.. University of Iowa; Estados Unido

Cell cycle regulation of a Xenopus Wee1-like kinase

Using a polymerase chain reaction-based strategy, we have isolated a gene encoding a Wee1-like kinase from Xenopus eggs. The recombinant Xenopus Wee1 protein efficiently phosphorylates Cdc2 exclusively on Tyr- 15 in a cyclin-dependent manner. The addition of exogenous Wee1 protein to Xenopus cell cycle extracts results in a dose-dependent delay of mitotic initiation that is accompanied by enhanced tyrosine phosphorylation of Cdc2. The activity of the Wee1 protein is highly regulated during the cell cycle: the interphase, underphosphorylated form of Wee1 (68 kDa) phosphorylates Cdc2 very efficiently, whereas the mitotic, hyperphosphorylated version (75 kDa) is weakly active as a Cdc2-specific tyrosine kinase. The down-modulation of Wee1 at mitosis is directly attributable to phosphorylation, since dephosphorylation with protein phosphatase 2A restores its kinase activity. During interphase, the activity of this Wee1 homolog does not vary in response to the presence of unreplicated DNA. The mitosis-specific phosphorylation of Wee1 is due to at least two distinct kinases: the Cdc2 protein and another activity (kinase X) that may correspond to an MPM-2 epitope kinase. These studies indicate that the down-regulation of Wee1-like kinase activity at mitosis is a multistep process that occurs after other biochemical reactions have signaled the successful completion of S phase

Multispectral techniques applied to photomicrography

Based upon an objects absorbtion and reflection characteristics to varying amounts of radiation, a series of selective wavelength band exposures were made for color separation of a microscopic specimen. The exposures axe made to record the specimens characteristic transmission or reflection patterns as a visible density value. The exposeures were made in a overlapping band width manner. The exposures were in the blue, green, and red regions of the spectrum. The resultant negetives were printed on Polycontrast paper using filter No. 2. The main purpose of the experiment was the design of a photographic system to remove the biological step of staining. This was through the use of the spectral transmission curves of the componet parts of the specimen. The results of the separations must than be referenced to the spectral output of the specimens componet parts. This was not tried because of the region of the spectrum that was needed was in the far ultraviolet

Development and Validation of ReaxFF Reactive Force Field for Hydrocarbon Chemistry Catalyzed by Nickel

To enable the study of hydrocarbon reactions catalyzed by nickel surfaces and particles using reactive molecular dynamics on thousands of atoms as a function of temperature and pressure, we have developed the ReaxFF reactive force field to describe adsorption, decomposition, reformation and desorption of hydrocarbons as they interact with the nickel surface. The ReaxFF parameters were determined by fitting to the geometries and energy surfaces from quantum mechanics (QM) calculations for a large number of reaction pathways for hydrocarbon molecules chemisorbed onto nickel (111), (100) and (110) surfaces, supplemented with QM equations of state for nickel and nickel carbides. We demonstrate the validity and accuracy of ReaxFF by applying it to study the reaction dynamics of hydrocarbons as catalyzed by nickel particles and surfaces. For the dissociation of methyl on the (111), (100), and stepped (111) surfaces of nickel, we observe the formation of chemisorbed CH plus subsurface carbide. We observe that the (111) surface is the least reactive, the (100) surface has the fastest reaction rates, and the stepped (111) surface has an intermediate reaction rate. The importance of surface defects in accelerating reaction rates is highlighted by these results

Structures, Energetics, and Reaction Barriers for CH_x Bound to the Nickel (111) Surface

To provide a basis for understanding and improving such reactive processes on nickel surfaces as the catalytic steam reforming of hydrocarbons, the decomposition of hydrocarbons at fuel cell anodes, and the growth of carbon nanotubes, we report quantum mechanics calculations (PBE flavor of density functional theory, DFT) of the structures, binding energies, and reaction barriers for all CH_x species on the Ni(111) surface using periodically infinite slabs. We find that all CH_x species prefer binding to μ3 (3-fold) sites leading to bond energies ranging from 42.7 kcal/mol for CH_3 to 148.9 kcal/mol for CH (the number of Ni-C bonds is not well-defined). We find reaction barriers of 18.3 kcal/mol for CH_(3,ad) → CH_(2,ad) + H_(ad) (with ΔE = +1.3 kcal/ mol), 8.2 kcal/mol for CH_(2,ad) → CH_(ad) + H_(ad) (with ΔE = -10.2 kcal/mol) and 32.3 kcal/mol for CH_(ad) → C_(ad) + H_(ad) (with ΔE = 11.6 kcal/mol). Thus, CH_(ad) is the stable form of CH_x on the surface. These results are in good agreement with the experimental data for the thermodynamic stability of small hydrocarbon species following dissociation of methane on Ni(111) and with the intermediates isolated during the reverse methanation process

Competing, Coverage-Dependent Decomposition Pathways for C_2H_y Species on Nickel (111)

Competing, coverage-dependent pathways for ethane (CH_3CH_3) decomposition on Ni(111) are proposed on the basis of quantum mechanics (QM) calculations, performed by using the PBE flavor of density functional theory (DFT), for all C_2H_y species adsorbed to a periodically infinite Ni(111) surface. For CH_2CH_3, CHCH_3, and CCH_3, we find that the surface C is tetrahedral in each case, with the surface C forming bonds to one, two, or three Ni atoms with bond energies scaling nearly linearly (E_(bond) = 32.5, 82.7, and 130.8 kcal/mol, respectively). In each of the remaining six C_2H_y species, both C atoms are able to form bonds to the surface. Three of these (CH_2CH_2, CHCH_2, and CCH_2) adsorb most favorably at a fcc-top site with the methylene C located at an on-top site and the other C at an adjacent fcc site. The bond energies for these species are E_(bond) = 19.7, 63.2, and 93.6 kcal/mol, respectively. The remaining species (CHCH, CCH, and C_2) all prefer binding at fcc-hcp sites, where the C atoms sit in a pair of adjacent fcc and hcp sites, with binding energies of E_(bond) = 57.7, 120.4, and 162.8 kcal/mol, respectively. We find that CHCH_(ad) is the most stable surface species (ΔH_(eth) = −18.6), and an important intermediate along the lowest-energy decomposition pathway for ethane on Ni(111). The second most stable species, CCH_3, is a close competitor (ΔH_(eth) = −18.2 kcal/mol), lying along an alternative decomposition pathway that is preferred for high-surface-coverage conditions. The existence of these competing, low- and high-coverage decomposition pathways is consistent with the experiments. The QM results reported here were used as training data in the development of the ReaxFF reactive force field describing hydrocarbon reactions on nickel surfaces [Mueller, J. E.; van Duin, A: C. T.; Goddard, W. A. J. Phys. Chem. C 2010, 114, 4939−4949]. This has enabled Reactive dynamics studying the chemisorption and decomposition of systems far too complex for quantum mechanics. Thus we reported recently, the chemisorption and decomposition of six different hydrocarbon species on a Ni_(468) nanoparticle catalysts using this ReaxFF description [Mueller, J. E.; van Duin, A: C. T.; Goddard, W. A. J. Phys. Chem. C 2010, 114, 5675−5685]