Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium

The aim of this work was to improve the production of fructosyltransferase (FTase) by Solid-State Fermentation (SSF) using aguamiel (agave sap) as culture medium and Aspergillus oryzae DIA-MF as producer strain. SSF was carried out evaluating the following parameters: inoculum rate, incubation temperature, initial pH and packing density to determine the most significant factors through Box-Hunter and Hunter design. The significant factors were then further optimized using a Box-Behnken design and response surface methodology. The maximum FTase activity (1347 U/L) was obtained at 32 °C, using packing density of 0.7 g/cm3. Inoculum rate and initial pH had no significant influence on the response. FOS synthesis applying the enzyme produced by A. oryzae DIA-MF was also studied using aguamiel as substrate.Authors thank National Council for Science and Technology (CONACYT) of Mexico by the financial support through the project No. CB-2011-C01-167764. Author D. B. Muñiz-Márquez also thank CONACYT for the financial support during her postgraduate program (Doctorate) in Food Science and Technology offered by the University Autonomous of Coahuila, Mexico

Fructo-oligosaccharides (FOS) production by fungal submerged culture using aguamiel as a low-cost by-product

Supplementary data to this article can be found online at https:// doi.org/10.1016/j.lwt.2018.12.020.Fructooligosaccharides (FOS) are important ingredients in the functional food industry because they have different biological properties such as decrease level of triglycerides, cholesterol and phospholipids and stimulate growth of probiotics for enhancement of microflora in large intestine. However, current strategies for the FOS production through simple and economical bioprocess has been necessary. The aim of this work was evaluated the capacity of three fungal strains (Aspergillus niger GH1, Aspergillus niger PSH and Aspergillus oryzae DIA-MF) to produce fructooligosaccharides (FOS) using aguamiel from Agave salmiana as an economical substrate. In addition, Czapek Dox medium supplemented with sucrose as carbon source was used as a control medium for the FOS production. A. oryzae DIA-MF was a fungi producer of FOS using aguamiel or Czapek Dox medium as substrate at 24?h of fermentation. However, the yield of FOS was increased two folds (20.30?g/L), with a productivity of 0.84?g FOS/l.h when aguamiel was used as substrate. On the other hand, A. niger GH1 and A. niger PSH showing only hydrolytic activity on sucrose under the studied conditions. In conclusion, this study shown excellent compatibility of A. oryzae DIA-MF using aguamiel as an economical substrate for the FOS production under a simple bioprocess.Authors thank all technical support and comments made by Dra. Adriana C. Flores-Gallegos and Dra. Abril Flores-Maltos. Authors thank National Council for Science and Technology (CONACYT) of Mexico by the financial support through the project No. CB-2011-C01-167764. Author D. B. Muñiz-Márquez also thank CONACYT for the financial support during her postgraduate program (Doctorate) in Food Science and Technology offered by the University Autonomous of Coahuila, Mexico.info:eu-repo/semantics/publishedVersio

Tendencias de la bioenergía: del metagenoma de hábitats ricos en azufre a la purificación del biogás

A large amount of wastes is generated by agro-industry and can be valorized to obtain useful products with higher added value, thus reducing environmental impact. There are alternatives to valorize these wastes and the production of bioenergy has been a great precedent, from the production of biodiesel, bioethanol and biogas that is possible by the use of biomass. Biogas production by methanogenesis is an alternative for the generating biofuels and energy. However, a problem arises during biogas production due to the presence of hydrogen sulfide (and other compounds), which is toxic and can damage the biogas plant (up concentrations of 658 ppmv), increase SOx emissions and inhibit the fermentation process of biogas production, so it is necessary to eliminate them. The biological removal method of this compound is included, by oxidization through microorganisms. The objective of this review is to expose the trends in the use of the microorganisms mentioned in environmental biotechnology, particularly their role in biogas purification.Gran cantidad de residuos generados por la agroindustria son considerados tanto para la obtención de productos útiles de mayor valor agregado como para reducir el impacto ambiental. Existen alternativas para evaluar estos desechos siendo la producción de bioenergía una solución de gran precedente, desde la producción de biodiesel, bioetanol y biogás hasta aprovechar la biomasa. La producción de biogás por metanogénesis es una alternativa para la generación de biocombustibles y energía. Sin embargo, surge un problema que puede generar daños en la planta industrial durante la producción del biogás, cuando se inhibe su proceso fermentativo por la presencia tóxica del sulfuro de hidrógeno y se presenta un aumento en la emisión de SOx (en concentraciones de hasta 658 ppmv). Dentro de los métodos de remoción de este compuesto se encuentra el biológico, a través de microorganismos por oxidación. El objetivo de esta revisión es exponer las tendencias del uso de microorganismos oxidantes de azufre en biotecnología ambiental, particularmente el papel de éstos en la purificación del biogás

Influence of thermal effect on sugars composition of Mexican Agave syrup

Agave syrup is a fermentable by-product from the Agave industry that is used for pulque production, a typical Mexican fermented beverage. However, to date, the information available on its physicochemical composition is scarce, with this study being one of the first contributions on the subject. Here the influence of thermal treatment at 121ºC/15 min on the physicochemical composition of agave syrup was studied. The chemical composition based on sugar content was evaluated by thin-layer chromatography and high-performance liquid chromatography. In addition, the mass spectrum is also presented. Results showed that thermal treatment promoted a change in the chemical composition of the agave syrup, particularly in sugar concentration favoring a high sucrose concentration after the sterilization process. Fructose, glucose in particular, and kestose (GF2) were detected in both samples. The presence of prebiotics such as GF2 suggests that agave syrup may be used in food and nutraceutical industries as a functional beverage.El jarabe de Agave es un subproducto típico fermentable de la industria del Agave, que es usado para la producción del pulque, una bebida fermentada mexicana. Sin embargo, hasta la fecha, la información disponible sobre su composición fisicoquímica es escasa, siendo esta una de las primeras contribuciones. Aquí, la influencia del tratamiento térmico a 15 libras/121ºC/15 min en la composición fisicoquímica del jarabe de Agave fue estudiada. La composición química basada en el contenido de azúcares fue evaluada por cromatografía en capa fina (TLC) y cromatografía líquida de alta resolución (HPLC). Además, el espectro de masas también es presentado. Los resultados muestran que el tratamiento térmico cambió la composición química del jarabe de Agave, particularmente en la concentración de azúcares. La sacarosa fue el carbohidrato presente en alta concentración después del proceso de esterilización. La fructosa, glucosa y en particular la kestosa (GF2) fueron detectadas en ambas muestras. La presencia de prebióticos tales como GF2 sugiere que el jarabe de Agave podría ser usado en las industrias de alimentos y farmacéuticas como una bebida funcional.The authors thank the National Council for Science and Technology (CONACYT) of Mexico for the financial support through project No. CB-2011-C01-167764. Authors D. B. Muniz-Marquez and J. E. Wong-Paz also thank CONACYT for the graduate scholarship for the postgraduate program (Doctorate) in Food Science and Technology offered by the University Autonomous of Coahuila, Mexico