2,929 research outputs found
Spatial heterogeneity and peptide availability determine CTL killing efficiency in vivo
The rate at which a cytotoxic T lymphocyte (CTL) can survey for infected cells is a key ingredient of models of vertebrate immune responses to intracellular pathogens. Estimates have been obtained using in vivo cytotoxicity assays in which peptide-pulsed splenocytes are killed by CTL in the spleens of immunised mice. However the spleen is a heterogeneous environment and splenocytes comprise multiple cell types. Are some cell types intrinsically more susceptible to lysis than others? Quantitatively, what impacts are made by the spatial distribution of targets and effectors, and the level of peptide-MHC on the target cell surface? To address these questions we revisited the splenocyte killing assay, using CTL specific for an epitope of influenza virus. We found that at the cell population level T cell targets were killed more rapidly than B cells. Using modeling, quantitative imaging and in vitro killing assays we conclude that this difference in vivo likely reflects different migratory patterns of targets within the spleen and a heterogeneous distribution of CTL, with no detectable difference in the intrinsic susceptibilities of the two populations to lysis. Modeling of the stages involved in the detection and killing of peptide-pulsed targets in vitro revealed that peptide dose influenced the ability of CTL to form conjugates with targets but had no detectable effect on the probability that conjugation resulted in lysis, and that T cell targets took longer to lyse than B cells. We also infer that incomplete killing in vivo of cells pulsed with low doses of peptide may be due to a combination of heterogeneity in peptide uptake and the dissociation, but not internalisation, of peptide-MHC complexes. Our analyses demonstrate how population-averaged parameters in models of immune responses can be dissected to account for both spatial and cellular heterogeneity
Kernelization and Parameterized Algorithms for 3-Path Vertex Cover
A 3-path vertex cover in a graph is a vertex subset such that every path
of three vertices contains at least one vertex from . The parameterized
3-path vertex cover problem asks whether a graph has a 3-path vertex cover of
size at most . In this paper, we give a kernel of vertices and an
-time and polynomial-space algorithm for this problem, both new
results improve previous known bounds.Comment: in TAMC 2016, LNCS 9796, 201
On Arnold's 14 `exceptional' N=2 superconformal gauge theories
We study the four-dimensional superconformal N=2 gauge theories engineered by
the Type IIB superstring on Arnold's 14 exceptional unimodal singularities
(a.k.a. Arnold's strange duality list), thus extending the methods of 1006.3435
to singularities which are not the direct sum of minimal ones. In particular,
we compute their BPS spectra in several `strongly coupled' chambers.
From the TBA side, we construct ten new periodic Y-systems, providing
additional evidence for the existence of a periodic Y-system for each isolated
quasi-homogeneous singularity with (more generally, for each N=2
superconformal theory with a finite BPS chamber whose chiral primaries have
dimensions of the form N/l).Comment: 73 pages, 7 figure
The temperature and chronology of heavy-element synthesis in low-mass stars
Roughly half of the heavy elements (atomic mass greater than that of iron)
are believed to be synthesized in the late evolutionary stages of stars with
masses between 0.8 and 8 solar masses. Deep inside the star, nuclei (mainly
iron) capture neutrons and progressively build up (through the
slow-neutron-capture process, or s-process) heavier elements that are
subsequently brought to the stellar surface by convection. Two neutron sources,
activated at distinct temperatures, have been proposed: 13C and 22Ne, each
releasing one neutron per alpha-particle (4He) captured. To explain the
measured stellar abundances, stellar evolution models invoking the 13C neutron
source (which operates at temperatures of about one hundred million kelvin) are
favoured. Isotopic ratios in primitive meteorites, however, reflecting
nucleosynthesis in the previous generations of stars that contributed material
to the Solar System, point to higher temperatures (more than three hundred
million kelvin), requiring at least a late activation of 22Ne. Here we report a
determination of the s-process temperature directly in evolved low-mass giant
stars, using zirconium and niobium abundances, independently of stellar
evolution models. The derived temperature supports 13C as the s-process neutron
source. The radioactive pair 93Zr-93Nb used to estimate the s-process
temperature also provides, together with the pair 99Tc-99Ru, chronometric
information on the time elapsed since the start of the s-process, which we
determine to be one million to three million years.Comment: 30 pages, 10 figure
Tunable few-electron double quantum dots and Klein tunnelling in ultra-clean carbon nanotubes
Quantum dots defined in carbon nanotubes are a platform for both basic
scientific studies and research into new device applications. In particular,
they have unique properties that make them attractive for studying the coherent
properties of single electron spins. To perform such experiments it is
necessary to confine a single electron in a quantum dot with highly tunable
barriers, but disorder has until now prevented tunable nanotube-based
quantum-dot devices from reaching the single-electron regime. Here, we use
local gate voltages applied to an ultra-clean suspended nanotube to confine a
single electron in both a single quantum dot and, for the first time, in a
tunable double quantum dot. This tunability is limited by a novel type of
tunnelling that is analogous to that in the Klein paradox of relativistic
quantum mechanics.Comment: 21 pages including supplementary informatio
Discrete Convex Functions on Graphs and Their Algorithmic Applications
The present article is an exposition of a theory of discrete convex functions
on certain graph structures, developed by the author in recent years. This
theory is a spin-off of discrete convex analysis by Murota, and is motivated by
combinatorial dualities in multiflow problems and the complexity classification
of facility location problems on graphs. We outline the theory and algorithmic
applications in combinatorial optimization problems
General Argyres-Douglas Theory
We construct a large class of Argyres-Douglas type theories by compactifying
six dimensional (2,0) A_N theory on a Riemann surface with irregular
singularities. We give a complete classification for the choices of Riemann
surface and the singularities. The Seiberg-Witten curve and scaling dimensions
of the operator spectrum are worked out. Three dimensional mirror theory and
the central charges a and c are also calculated for some subsets, etc. Our
results greatly enlarge the landscape of N=2 superconformal field theory and in
fact also include previous theories constructed using regular singularity on
the sphere.Comment: 55 pages, 20 figures, minor revision and typos correcte
Endothelin-1 Predicts Hemodynamically Assessed Pulmonary Arterial Hypertension in HIV Infection.
BackgroundHIV infection is an independent risk factor for PAH, but the underlying pathogenesis remains unclear. ET-1 is a robust vasoconstrictor and key mediator of pulmonary vascular homeostasis. Higher levels of ET-1 predict disease severity and mortality in other forms of PAH, and endothelin receptor antagonists are central to treatment, including in HIV-associated PAH. The direct relationship between ET-1 and PAH in HIV-infected individuals is not well described.MethodsWe measured ET-1 and estimated pulmonary artery systolic pressure (PASP) with transthoracic echocardiography (TTE) in 106 HIV-infected individuals. Participants with a PASP ≥ 30 mmHg (n = 65) underwent right heart catheterization (RHC) to definitively diagnose PAH. We conducted multivariable analysis to identify factors associated with PAH.ResultsAmong 106 HIV-infected participants, 80% were male, the median age was 52 years and 77% were on antiretroviral therapy. ET-1 was significantly associated with higher values of PASP [14% per 0.1 pg/mL increase in ET-1, p = 0.05] and PASP ≥ 30 mmHg [PR (prevalence ratio) = 1.24, p = 0.012] on TTE after multivariable adjustment for PAH risk factors. Similarly, among the 65 individuals who underwent RHC, ET-1 was significantly associated with higher values of mean pulmonary artery pressure and PAH (34%, p = 0.003 and PR = 2.43, p = 0.032, respectively) in the multivariable analyses.ConclusionsHigher levels of ET-1 are independently associated with HIV-associated PAH as hemodynamically assessed by RHC. Our findings suggest that excessive ET-1 production in the setting of HIV infection impairs pulmonary endothelial function and contributes to the development of PAH
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