Effects of soy protein base diet supplemented with lysine and methionine on digestive enzymes activity and hematological parameters in silvery-black porgy (Sparidentex hasta) juveniles

The effect of dietary partial replacement of fish meal (FM) by soybean protein (SP) alone or in combination with lysine (Lys) and methionine (Met) supplementation were tested in a 60-days feeding trial for silvery-black porgy (Sparidentex hasta) juveniles. Seven isoproteic (ca. 50% crude protein) and isoenergetic (ca. 22.4 MJ/kg) diets were formulated in which 45% (SP45), 60% (SP60) and 75% (SP75) of FM protein were replaced by SP and the control diet (FM) was prepared with FM as the major source of protein. In SP45+, SP60+ and SP75+ diets, 45 to 75% of FM was replaced by SP with supplementing blends of Lys and Met (98% of purity). The activities of the trypsin, lipase and α-amylase were higher in fish fed SP diets with crystalline amino acids supplementation than in the other groups (P < 0.05). Fish fed SP75 and SP75+ diets had the lowest red blood cell count and hematocrit level (P < 0.05). The results of the current study indicated that anti-nutritional factors in a soy-protein based diet rather than lysine and methionine deficiencies may have adverse effects on digestive enzymes activities and health condition in silvery-black porgy juveniles.info:eu-repo/semantics/publishedVersio

Lysine and methionine supplementation in high soy protein content diets for silvery-black porgy (Sparidentex hasta) juveniles

A two-month feeding trial was conducted to evaluate the effect of partial replacement of fish meal (FM) by soybean protein (SP) alone or in combination with lysine (Lys) and methionine (Met) supplementation in practical diets for silvery-black porgy juveniles (16.7±0.1 g). Seven isoproteic (ca. 50% crude protein) and isoenergetic (ca. 22.4 MJ kg-1) diets were formulated in which 45% (SP45), 60% (SP60) and 75% (SP75) of FM protein were replaced by SP and the control diet (FM) was prepared with FM as the major source of protein. In SP45+, SP60+ and SP75+ diets, 45 to 75% of FM was replaced by SP with supplementing blends of Lys and Met. Growth performance, feed utilization, and protein and lipid digestibility decreased with increasing dietary SP levels (p<0.05). Fish fed SP75 had the highest whole body lipid content, but with the lowest whole body arginine, lysine, histidine, phenylalanine and taurine concentrations (p<0.05). Supplementing Lys and Met in SP based diets did not improve growth performance, suggesting that the anti-nutritional factors in soybean protein products rather than Met and Lys deficiency might limit FM substitution with SP in silvery-black porgy juveniles.info:eu-repo/semantics/publishedVersio

Effects of feeding rate on growth and feed efficiency of Sobaity seabream (Sparidentex hasta) fry in nursery phase

A 30-day research was conducted to evaluate the effects of different feeding rate (formulated diet) (2, 4, 6, 8 and 10% of wet body weight/day) and also fresh food (20% of wet body weight/day) on growth performance and feed utilization of Sparidentex hasta fry in nursery phase. Growth performance including final weight and specific growth rate (SGR) significantly increased with increasing feeding rate (p<0.05). The relationship between SGR (% per day) and ration level (% per day) was an asymptotic curve described as SGR = 0.2809X+1.4191. Feed efficiency ratio (FER) decreased significantly with increasing ration levels, and the relationship between FER and ration level (% per day) described as FER =-0.0586X+1.3184. Hepatosomatic index significantly increased with increasing feeding rate (p<0.05). Based on the above results, it may be concluded that a ration size of 8% of body weight per day is optimal for good growth and feed conversion efficiency of juvenile S. hasta in nursery phase

Growth performance and energy budget of the yellowfin seabream, Acanthopagrus arabicus (Iwatsuki, 2013) affected by the combined effects of water temperature and salinity

A 56-day research was carried out to determine the interactive effects of water temperature (WT) and salinity (WS) on physiological responses of yellowfin seabream, Acanthopagrus arabicus (Iwatsuki, 2013) (10.0}0.2 g). Three salinities (S; 12, 35 and 50 ñ) and four temperatures (T; 16, 23, 30 and 35‹C) were selected to design 12 experimental treatments. The husbandry system consisted of thirty-six 300-L cylindrical polyethylene tanks that were filled with 250 L of water. Each treatment had three replicates (tanks) containing 20 fish in each tank. Fish were handfed on a commercial feed (440 g kg-1 crude protein and 180 g kg-1 crude fat) twice daily at visual satiation. Five fish from each tank were transferred into an experimental chamber to evaluate their oxygen consumption and ammonia excretion. There was a positive correlation between fish oxygen consumption and WT (r=0.594; p=0.001). In addition, there was a negative relationship between WS and ammonia excretion (AE) (r = -0.865; p=0.029), meanwhile the relationship between WT and AE was positive (r=0.422; P=0.01). Growth parameters were significantly affected by WT, WS and their interactions so that the highest final body was recorded in the highest temperature and salinity treatment. On the other hand, the lowest body weight was observed in the lowest WT (i.e., 16Ž) irresponsive to WS. The moisture level in the whole body was affected by WT (p=0.012). The crude lipid in the whole body was influenced by only WS (p=0.04). The ash content in the whole body was profoundly affected by WT (p=0.0001), WS (p=0.0001) and their interaction effect (p=0.04). The energy content of the whole body was only affected by WT (p=0.025). The energy budget of fish was influenced by WT (p=0.0001) and its interaction with WS (p=0.015). In summary, the present findings indicated that the preferred WT for A. arabicus is between 30 to 35‹C in brackish water

Legumes Research Volume 2

Hekmatpour, F. et al. - Jiménez-López, José Carlos (CSIC); Clemente, Alfonso (CSIC) Editor

Effects of dietary fern (Adiantum capillus-veneris) leaves powder on serum and mucus antioxidant defence, immunological responses, antimicrobial activity and growth performance of common carp (Cyprinus carpio) juveniles

A 56-day research was performed to examine the influence of graded levels (0 (control), 0.5, 1 and 2) of Fern (Adiantum capillus-veneris) leaves powder (FLP) in diet on immune competence and growth of common carp (Cyprinus carpio, initial weight = 20 g). The serum total immunoglobulins content and lysozyme activity in the 1 and 2 FLP groups remarkably increased compared to the other groups (P 0.05). The serum bactericidal activity against human and fish pathogens increased with enhancing the FLP level in diet against Staphylococcus aureus, Escherichia coli (EHEC ATCC 43895), Escherichia coli (CI), Pseudomonas aeruginosa, Klebsiella pneumonia and Aeromonas hydrophila. The serum antibacterial activity against Yersinia ruckeri in the 2 FLP group was higher than the other treatments. Furthermore, the serum bactericidal activity against P. aeruginosa (ATCC 27853) only observed in fish fed on the 1 and 2 FLP-supplemented diets. The skin mucosal bactericidal activity and inhibitory effects increased with enhancing the FLP level in diet against E. coli, K. pneumonia, Y. ruckeri and A. hydrophila in a dose response manner. Moreover, the skin mucosal bactericidal activity against S. aureus only observed in fish fed on 1 and 2 FLP-supplemented diets. The weight gain values in the 1 and 2 FLP groups were higher than the other treatments (P < 0.05). Feed conversion ratio (FCR) improved with increasing FLP level in diet in a dose-response manner (P < 0.05). By considering serum and mucosal bactericidal activities against different pathogenic bacteria, the supplementation of 2 FLP in diet is recommended for C. carpio during grow-out phase. © 2020 Elsevier Lt

Enhanced mucosal immune responses, immune related genes and growth performance in common carp (Cyprinus carpio) juveniles fed dietary Pediococcus acidilactici MA18/5M and raffinose

The present study was conducted to evaluate the effects of dietary Pediococcus acidilactici (PA) and raffinose (RF) alone or in combination on growth performance, mucosal immune factors and immune related genes expression in common carp (Cyprinus carpio) juveniles. Fish with initial weight of 10.0 ± 2.5 g were fed the following experimental diets for 60 days: control (without supplementation), prebiotic (2 g RF kg −1 diet), probiotic (6 × 10 8 CFU g −1 PA) and synbiotic (2 g RF kg −1 + 6 × 10 8 CFU g −1 PA). Carp fed synbiotic and probiotic diets had the highest (19.53 ± 0.16) and the lowest (18.05 ± 0.65) final body weight, respectively and the other experimental groups showed intermediate values. Singular administration of PA or in combination with 2 g RF kg −1 significantly increased skin mucus total immunoglobulin (Ig) and protein compared other groups, meanwhile, values of skin mucus protease activity enhanced by dietary immunostimulants administration in comparison with the control (P &lt; 0.05). The expression of gene encoding lysozyme in skin pronouncedly increased by supplementing diets with singular or mixture of PA and RF; however, the expression of intestinal lysozyme gene as well as tumor necrosis factor-α genes expression in skin and intestine were not affected by administrating different immunostimulants (P &gt; 0.05). The highest growth performance was noticed in fish fed synbiotic (P &lt; 0.05). Overall, the combination of 2 g RF kg −1 with 6 × 10 8 CFU g −1 PA is recommended for improving immunological responses of C. carpio juveniles. © 2019 Elsevier Lt

Humoral and skin mucosal immune parameters, intestinal immune related genes expression and antioxidant defense in rainbow trout (Oncorhynchus mykiss) fed olive (Olea europea L.) waste

A six-week feeding trial was carried out to evaluate the effects of inclusion of dietary olive waste cake (OWC, 0, 0.5, 2.5 and 5 g kg−1 diet) on performance, antioxidant condition and immune responses of rainbow trout (Oncorhynchus mykiss) (2.5 ± 0.1 g). Supplementing diet with 2.5 and 5 g OWC kg−1 diet significantly enhanced serum and mucosal lysozyme activity in fish. Regarding mucosal immunity, fish fed 2.5 and 5 g OWC kg−1 diets had higher skin mucus total Ig concentrations than other groups. In relation to antioxidant status, those in 2.5 g OWC kg−1 and the control groups exhibited the highest and the least liver superoxide dismutase and glutathione peroxidase activities, respectively. Furthermore, the activity of liver glutathione S transferase in fish fed 2.5 and 5 g OWC kg−1 diets was higher than the other treatments. In respect to gut cytokines gene expression, our findings demonstrated dietary OWC did not influence interlukines-1β and 10 genes expression, but relative expression of IL8 gene gradually up-regulated with increasing dietary OWC level. Moreover, fish fed 0.5 g OWC kg−1 and the control diets had the highest and the lowest gut tumor necrosis factor-α gene expression values, respectively. The relative expression of transforming growth factor-β significantly down-regulated in gut of fish fed 2.5 and 5 g OWC kg−1 diets compared to other groups. Supplementing diet with OWC pronouncedly improved growth and feed conversion ratio in fish compared to the control. Overall, the findings of this study suggested that inclusion of 2.5 g OWC kg−1 diet can improve growth rate, oxidative stress status, humoral and skin mucosal immune responses in O. mykiss fingerlings and it can be considered as a functional feed additive for this species. © 2020 Elsevier Lt

Effects of dietary fern (Adiantum capillus-veneris) leaves powder on serum and mucus antioxidant defence, immunological responses, antimicrobial activity and growth performance of common carp (Cyprinus carpio) juveniles

A 56-day research was performed to examine the influence of graded levels (0 (control), 0.5, 1 and 2%) of Fern (Adiantum capillus-veneris) leaves powder (FLP) in diet on immune competence and growth of common carp (Cyprinus carpio, initial weight = 20 g). The serum total immunoglobulins content and lysozyme activity in the 1 and 2% FLP groups remarkably increased compared to the other groups (P 0.05). The serum bactericidal activity against human and fish pathogens increased with enhancing the FLP level in diet against Staphylococcus aureus, Escherichia coli (EHEC ATCC 43895), Escherichia coli (CI), Pseudomonas aeruginosa, Klebsiella pneumonia and Aeromonas hydrophila. The serum antibacterial activity against Yersinia ruckeri in the 2% FLP group was higher than the other treatments. Furthermore, the serum bactericidal activity against P. aeruginosa (ATCC 27853) only observed in fish fed on the 1 and 2% FLP-supplemented diets. The skin mucosal bactericidal activity and inhibitory effects increased with enhancing the FLP level in diet against E. coli, K. pneumonia, Y. ruckeri and A. hydrophila in a dose response manner. Moreover, the skin mucosal bactericidal activity against S. aureus only observed in fish fed on 1 and 2% FLP-supplemented diets. The weight gain values in the 1 and 2% FLP groups were higher than the other treatments (P < 0.05). Feed conversion ratio (FCR) improved with increasing FLP level in diet in a dose-response manner (P < 0.05). By considering serum and mucosal bactericidal activities against different pathogenic bacteria, the supplementation of 2% FLP in diet is recommended for C. carpio during grow-out phase. © 2020 Elsevier Lt