Reduced Phagocytic Capacity of Blood Monocyte/Macrophages in Tuberculosis Patients Is Further Reduced by Smoking.

Tuberculosis (TB) and tobacco use are two major alarming global health issues posing immense threats to human populations. Mycobacterium tuberculosis (MTB) by activation of macrophages could induce the sequences of cells activation and releases of inflammatory cytokines such as CXCL-8, Il-12 and TNF-α which in turn induces the immune system network. However no information is available on other activity of cells by MTB and smoking. In the current study we aimed to investigate the serum levels TNF-a, CXCL-8 and phagocytosis capacity in tuberculosis patients with and without smoking. 103 subjects entered the study including 61 new diagnosed pulmonary TB patients (23 smokers and 38 nonsmokers) and 42 control healthy subjects. The phagocytosis of fluorescein isothiocyanate dextran (FITC-dextran) in blood monocytes/macrophages through flowcytometry was assessed. Serum levels of TNF-a and CXCL-8 were analyzed by ELISA methods. A lower percentage of cells from TB patients who smoked [50.29% (43.4-57.2), p<0.01] took up FITC-dextran after 2h compared to non-smoking TB subjects [71.62% (69.2-74.1)] and healthy cases [97.45% (95.9-99.1). Phagocytic capacity was inversely correlated with cigarette smoking as measured by pack years (r=-0.73, p<0.001). The serum levels of TNF-a and CXCL-8 were significantly higher in the TB patients who smoked compared to the TB non-smoker group (p<0.001, p<0.01 respectively). Blood monocytes/macrophages from TB patients have reduced phagocytic capacity which is further reduced in TB patients who smoke. Smoking enhanced serum levels of TNF-a and CXCL-8 suggesting a greater imbalance between the proinflammatory and anti-inflammatory factors in these patients

Inter-simple sequence repeats (ISSR) and morphological diversity in Onosma L. (Boraginaceae) species in Iran

Morphological characteristics as well as inter-simple sequence repeats (ISSR) molecular markers were used to study the species relationship in Onosma species (Boraginaceae) in Iran. Principal component analysis (PCA) showed that morphological characters like caule leaf size and shape, bract shape, corolla shape, nutlet length, venation and corolla teeth size and anther length are the most variable morphological characters among Onosma species studied. Out of 17 ISSR primers used, 6 primers produced 41 polymorphic and reproducible bands. Few specific ISSR bands were obtained for the species studied which show genetic material change during species diversification. Such bands may be used in the species identification too. Morphological and molecular trees obtained partly agreed with each other. In both trees, Onosma dasytrichum and Onosma microcarpum are placed close to each other, Onosma procerum and Onosma pachypodum show affinity to each other and Onosma araraticum, Onosma bodeanum, Onosma bistonensis, Onosma stenosiphon and  Onosma bulbotrichum are placed close to each other while, Onosma rostellatum stands far from the other species. The combined morphological and ISSR tree obtained separated the members of three sections of Onosma, Podnosma and Protonosma from each other. Moreover, almost a good separation of different subsections in the section Onosma was observed in the combined tree.Key words: Inter-simple sequence repeats, morphometry, Onosma

Association of dietary patterns with continuous metabolic syndrome in children and adolescents; A nationwide propensity score-matched analysis: The CASPIAN-V study

Objective: This study aims to determine the association of dietary patterns with metabolic syndrome (MetS) and its components in children and adolescents. Methods: This nationwide study was conducted in 2015 among 4200 students aged 7-18 years, who lived in 30 provinces in Iran. The analysis was conducted based on the propensity score using a matched case-control study design. Three dietary patterns were obtained conducting a principal component analysis with a varimax rotation on 16 dietary groups. Continuous MetS score was computed by standardizing the residuals (z-scores) of MetS components by regressing them according to age and sex. The gold standard diagnosis of MetS was considered based on the International Diabetes Federation criteria. Moreover, for the purpose of data analysis, matched logistics analysis was used. Results: The study participants consisted of 3843 children and adolescents (response rate 91.5) with mean (SD) age of 12.45 (3.04) years. Totally 49.4 of students were girls and 71.4 lived in urban areas. Three dietary patterns were obtained: Healthy, Western, and Sweet. Prevalence of MetS was 5 (boy 5.5 and girl 4.5). Results of multivariate analysis show that students with Sweet dietary patterns were at higher risk for abdominal obesity (OR 1.29; 95 CI 1.01-1.66), elevated blood pressure (OR 1.35; 95 CI 1.01-1.81) and MetS (OR 1.33; 95 CI 1.02-1.74). The two other dietary patterns were not associated with MetS and its components. Conclusion: Sweet dietary pattern increase the risk of MetS and some its components in Iranian children and adolescents. This finding provides valuable information for effective preventive strategies of MetS based on diet rather than medication to maintain healthy lifestyle habits. © 2018 The Author(s)

Economic inequality in prevalence of underweight and short stature in children and adolescents: The weight disorders survey of the caspian-IV study

Objective: The aim of this study was to determine the determinants of socio-economic inequality in the prevalence of short stature and underweight in Iranian children and adolescents. Subjects and methods: This cross-sectional nationwide study was conducted on 36,486 participants, aged 6-18 years. This school-based surveillance (CASPIAN-IV) program and its complementary part on weight disorders evaluation was conducted in urban and rural areas of 30 provinces in Iran. In addition to physical examination, a validated questionnaire was completed from students and their parents. Socio-economic status (SES) was determined using principal component analysis, and was classified in quintile scale. Inequality in the prevalence of underweight and short stature was assessed using concentration (C) index and slop index of inequality (SII) by the Oaxaca-Blinder decomposition method. Results: The prevalence (95 CI) of underweight and short stature at national level was 10.89 (10.55, 11.23) and 4.15 (3.94, 4.38), respectively; it had a downtrend from the lowest to highest SES quintile. Furthermore, the value of C for underweight and short stature was negative, i.e. inequality was in favor of high SES groups. Moreover, the prevalence gap of underweight and short stature in the first and fifth quintiles of SES was 6.58 and 5.80, respectively. The highest proportion of this gap was explained by living area. In the multiple logistic model, odds of underweight and short stature were significantly lower in individuals with higher SES. Compared to boys, odds of underweight were decreased in girls, whereas odds of short stature were increased in them. Odds of underweight and short stature were increased in participants from rural areas than in urban areas. With increasing age, the odds of underweight and short stature decreased significantly. Conclusions: The results of this study showed that inequality in the prevalence of short stature and underweight was in favor of high SES groups. Moreover, living area was one of the most important determinants that explained this inequality. Therefore, this issue needs to be considered in health promotion policies. © 2020, Sociedade Brasileira de Endocrinologia e Metabologia. All rights reserved

Women's right to health in Iran

In Iran, discrimination based on gender in enjoyment of the right to health is prohibited. Making health services physically and financially accessible to the entire population and removing social and cultural barriers of women's access to health services are main considerations of the health law sand policies of Iran. The health of Iranian women has improved considerably in recent years. But there are disparities in health status and access of women to health services around the country. Some groups of women, including the poor, the elderly, the disabled, the illegal immigrant, and those without an appropriate male guardian, and rural women have limited access to health services in Iran. To realize women's right to health, this country should immediately remove the disparities and use all the necessary means including legislative, administrative, budgetary, promotional,and judicial measures. National plans on women's empowerment and support should be interpreted in provincial pro-grams and action plans. Moreover, a monitoring system and certain benchmarks for tracing the progress of the plans should be established. Realizing other economic, social,and cultural rights including the rights to food, shelter, education, work, social security, and participation in society will improve the Iranian women's enjoyment of their right

Beneficial Effects of Selenium on Some Morphological and Physiological Trait of Hot Pepper (Capsicum anuum)

Introduction: Aluminum (Al), cobalt (Co), sodium (Na), selenium (Se), and silicon (Si) are considered as beneficial elements for plants. They are not required for all plants but they can improve the growth and development of some plant species. Selenium is an essential element for human with antioxidant and antivirus functions but is not considered essential for higher plants. Selenium is reported to be protective against cancer and more than 40 types of diseases are associated with Se deficiency. The amounts of selenium in food also depend on the amount of the element in the soil. However, its beneficial role in improving plant growth and stress tolerances is well established. Plants revealed different physiological reactions into the Se levels, some specious accumulate it unlike some which are sensitive and Se is a toxic element for them. Some studies showed that Se can reduce adverse effects of salinity, drought, high and low temperatures and also heavy metal stress by enhancing antioxidant defense and MG detoxification systems. Pepper is one of the most important vegetable crops which have strong antioxidant properties. The effect of Se on vegetable especially on hot pepper is not well documented. Materials and Methods: Present experiment was designed in order to study the effects of different concentrations of selenium on vegetative growth and physiological trait of hot pepper (Capsicum annum cv. kenya) in hydroponic conditions in the greenhouse at the Department of Horticulture Science, Islamic Azad University of Shiraz (Iran) under natural light with a day/night average temperature of 25/17 °C, relative humidity of 50±8.5% and photoperiod 14/10 (day/night). This experiment was carried out based on completed randomized design (CRD) with 5 Se levels at (0 as control, 3, 5, 7 and 10 µM) with 3 replications. 30 days old seedling with uniform size were selected and transplanted into 4 L pot containing a mixture of peat moss and perlite (1:1). The nutrient solution was a modified Hoagland’s solution and each plant received 400 mL nutrient daily. Selenium concentrations were added by 0, 3, 7 µM Na2SeO3 (Merck, Germany) 10 days after transplanting. Leaf number (by counting), leaf area (with leaf area meter), relative water content (RWC), membrane stability index (MSI), chlorophyll and carotenoids content were determined one month after treatment applications. All data were subjected to one-way ANOVA by Statistix 8 (Tallahassee FL, USA) and the means were compared for significance by the least significant difference (LSD) test at p < 0.05. Results and Discussion: The results showed that selenium supplement at 5 µM significantly increased relative water content by 12.8% compared to control. Se increased root growth and thus absorbed more water and increased RWC in present experiment. Selenium at 7 and 10 µM Se increased membrane stability index by 33.61 and 80.06, respectively, compared to control which may be due to increasing potassium by Se application. Selenium at 3 and 5 µM increased leaf area by 24.6% and 25.1% relation to plants which were grown without Se supplementation. Leaf number increased by application of 5 µM Se about 15.14% and chlorophyll a, b and total increased by 64.67%, 38.5% and, 55.8%, respectively, in comparison with plants grown without Se application. Carotenoid content was not affected by different Se concentrations. Se increase chlorophyll content by increasing Mg and Fe absorption, or protect of chlorophyll content against chloroplast enzymes. The highest leaf area was observed at 3 µM Se in comparison with other treatments. Se at 5 µM is beneficial for growth, photosynthesis pigments, and leaf area and leaf number. Se application had no beneficial influence on lateral shoot. The highest starch concentration was observed at 5 µM Se in comparison with other treatments and control (by about 60% in comparison with plants were grown without Se application). Conclusion: In general, the result of present study indicated that selenium at low concentrations improved plant growth (such as leaf area and leaf number), physiological trait (relative water content and membrane stability index) and photosynthesis pigments (chlorophyll a and b) of hot pepper in hydroponic conditions. Also, it is suggested that this experiment should be repeated on some other important vegetable during growth and reproductive stages in hydroponic and soil condition and also on hot pepper quality and quantity of fruit