910 research outputs found
The main transition in the Pink membrane model: finite-size scaling and the influence of surface roughness
We consider the main transition in single-component membranes using computer
simulations of the Pink model [D. Pink {\it et al.}, Biochemistry {\bf 19}, 349
(1980)]. We first show that the accepted parameters of the Pink model yield a
main transition temperature that is systematically below experimental values.
This resolves an issue that was first pointed out by Corvera and co-workers
[Phys. Rev. E {\bf 47}, 696 (1993)]. In order to yield the correct transition
temperature, the strength of the van der Waals coupling in the Pink model must
be increased; by using finite-size scaling, a set of optimal values is
proposed. We also provide finite-size scaling evidence that the Pink model
belongs to the universality class of the two-dimensional Ising model. This
finding holds irrespective of the number of conformational states. Finally, we
address the main transition in the presence of quenched disorder, which may
arise in situations where the membrane is deposited on a rough support. In this
case, we observe a stable multi-domain structure of gel and fluid domains, and
the absence of a sharp transition in the thermodynamic limit.Comment: submitted to PR
Getting More from Pushing Less: Negative Specific Heat and Conductivity in Non-equilibrium Steady States
For students familiar with equilibrium statistical mechanics, the notion of a
positive specific heat, being intimately related to the idea of stability, is
both intuitively reasonable and mathematically provable. However, for system in
non-equilibrium stationary states, coupled to more than one energy reservoir
(e.g., thermal bath), negative specific heat is entirely possible. In this
paper, we present a ``minimal'' system displaying this phenomenon. Being in
contact with two thermal baths at different temperatures, the (internal) energy
of this system may increase when a thermostat is turned down. In another
context, a similar phenomenon is negative conductivity, where a current may
increase by decreasing the drive (e.g., an external electric field). The
counter-intuitive behavior in both processes may be described as `` getting
more from pushing less.'' The crucial ingredients for this phenomenon and the
elements needed for a ``minimal'' system are also presented.Comment: 14 pages, 3 figures, accepted for publication in American Journal of
Physic
Cluster phases of membrane proteins
A physical scenario accounting for the existence of size-limited
submicrometric domains in cell membranes is proposed. It is based on the
numerical investigation of the counterpart, in lipidic membranes where proteins
are diffusing, of the recently discovered cluster phases in colloidal
suspensions. I demonstrate that the interactions between proteins, namely
short-range attraction and longer-range repulsion, make possible the existence
of stable small clusters. The consequences are explored in terms of membrane
organization and diffusion properties. The connection with lipid rafts is
discussed and the apparent protein diffusion coefficient as a function of their
concentration is analyzed.Comment: 5 pages - enhanced versio
Judging Ordinary Meaning
Judges generally begin their interpretive task by looking for the ordinary meaning of the language of the law. And they often end there - out of respect for the notice function of the law or deference to the presumed intent of the lawmaker.
Most everyone agrees on the primacy of the ordinary meaning rule. Yet scholars roundly bemoan the indeterminacy of the communicative content of the language of the law. And they pivot quickly to other grounds for interpretation
From supported membranes to tethered vesicles: lipid bilayers destabilisation at the main transition
We report results concerning the destabilisation of supported phospholipid
bilayers in a well-defined geometry. When heating up supported phospholipid
membranes deposited on highly hydrophilic glass slides from room temperature
(i.e. with lipids in the gel phase), unbinding was observed around the main gel
to fluid transition temperature of the lipids. It lead to the formation of
relatively monodisperse vesicles, of which most remained tethered to the
supported bilayer. We interpret these observations in terms of a sharp decrease
of the bending rigidity modulus in the transition region, combined
with a weak initial adhesion energy. On the basis of scaling arguments, we show
that our experimental findings are consistent with this hypothesis.Comment: 11 pages, 3 figure
Theoretical investigation of finite size effects at DNA melting
We investigated how the finiteness of the length of the sequence affects the
phase transition that takes place at DNA melting temperature. For this purpose,
we modified the Transfer Integral method to adapt it to the calculation of both
extensive (partition function, entropy, specific heat, etc) and non-extensive
(order parameter and correlation length) thermodynamic quantities of finite
sequences with open boundary conditions, and applied the modified procedure to
two different dynamical models. We showed that rounding of the transition
clearly takes place when the length of the sequence is decreased. We also
performed a finite-size scaling analysis of the two models and showed that the
singular part of the free energy can indeed be expressed in terms of an
homogeneous function. However, both the correlation length and the average
separation between paired bases diverge at the melting transition, so that it
is no longer clear to which of these two quantities the length of the system
should be compared. Moreover, Josephson's identity is satisfied for none of the
investigated models, so that the derivation of the characteristic exponents
which appear, for example, in the expression of the specific heat, requires
some care
A Visual Pathway Links Brain Structures Active during Magnetic Compass Orientation in Migratory Birds
The magnetic compass of migratory birds has been suggested to be light-dependent. Retinal cryptochrome-expressing neurons and a forebrain region, “Cluster N”, show high neuronal activity when night-migratory songbirds perform magnetic compass orientation. By combining neuronal tracing with behavioral experiments leading to sensory-driven gene expression of the neuronal activity marker ZENK during magnetic compass orientation, we demonstrate a functional neuronal connection between the retinal neurons and Cluster N via the visual thalamus. Thus, the two areas of the central nervous system being most active during magnetic compass orientation are part of an ascending visual processing stream, the thalamofugal pathway. Furthermore, Cluster N seems to be a specialized part of the visual wulst. These findings strongly support the hypothesis that migratory birds use their visual system to perceive the reference compass direction of the geomagnetic field and that migratory birds “see” the reference compass direction provided by the geomagnetic field
Vesicle shape, molecular tilt, and the suppression of necks
Can the presence of molecular-tilt order significantly affect the shapes of
lipid bilayer membranes, particularly membrane shapes with narrow necks?
Motivated by the propensity for tilt order and the common occurrence of narrow
necks in the intermediate stages of biological processes such as endocytosis
and vesicle trafficking, we examine how tilt order inhibits the formation of
necks in the equilibrium shapes of vesicles. For vesicles with a spherical
topology, point defects in the molecular order with a total strength of
are required. We study axisymmetric shapes and suppose that there is a
unit-strength defect at each pole of the vesicle. The model is further
simplified by the assumption of tilt isotropy: invariance of the energy with
respect to rotations of the molecules about the local membrane normal. This
isotropy condition leads to a minimal coupling of tilt order and curvature,
giving a high energetic cost to regions with Gaussian curvature and tilt order.
Minimizing the elastic free energy with constraints of fixed area and fixed
enclosed volume determines the allowed shapes. Using numerical calculations, we
find several branches of solutions and identify them with the branches
previously known for fluid membranes. We find that tilt order changes the
relative energy of the branches, suppressing thin necks by making them costly,
leading to elongated prolate vesicles as a generic family of tilt-ordered
membrane shapes.Comment: 10 pages, 7 figures, submitted to Phy. Rew.
Cryptochromes and neuronal-activity markers colocalize in the retina of migratory birds during magnetic orientation
Migratory birds can use a magnetic compass for orientation during their migratory journeys covering thousands of kilometers. But how do they sense the reference direction provided by the Earth’s magnetic field? Behavioral evidence and theoretical considerations have suggested that radical-pair processes in differently oriented, light-sensitive molecules of the retina could enable migratory birds to perceive the magnetic field as visual patterns. The cryptochromes (CRYs) have been suggested as the most likely candidate class of molecules, but do CRYs exist in the retina of migratory birds? Here, we show that at least one CRY1 and one CRY2 exist in the retina of migratory garden warblers and that garden-warbler CRY1 (gwCRY1) is cytosolic. We also show that gwCRY1 is concentrated in specific cells, particularly in ganglion cells and in large displaced ganglion cells, which also showed high levels of neuronal activity at night, when our garden warblers performed magnetic orientation. In addition, there seem to be striking differences in CRY1 expression between migratory and nonmigratory songbirds at night. The difference in CRY1 expression between migrants and nonmigrants is particularly pronounced in the large displaced ganglion cells known to project exclusively to a brain area where magnetically sensitive neurons have been reported. Consequently, cytosolic gwCRY1 is well placed to possibly be the primary magnetic-sensory molecule required for light-mediated magnetoreception
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