Design of a Self-Resetting, Low-Maintenance, Long-Term Bait Station for Rodent Control

A low-maintenance, long-term bait station that resets itself after being triggered would be a very useful tool for controlling Richardson’s ground squirrels, or other problem rodent species, in remote locations. With collaborators, we developed and tested two such devices using lab rats in pen settings. The devices can be left in-situ for long periods of time without servicing, and requires only occasional bait and/or battery replacement. Squirrels would be unable to cache bait due to the integrated time-out mechanism. The devices use capacitive sensor or strain gauge systems for animal identification, making it very unlikely that smaller non-target species would be able to trigger the systems while the design precludes entry by larger non-target species. Further refinement and testing will be needed before a viable, commercial product can go into production. These refinements include increasing reliability, reducing power requirements, design features and triggering mechanisms tightly linked to the attributes of the targeted pest species, and reduction of production costs. The devices will also need to be tested in field settings for extended periods of time

Evaluation of a Food Bait Block for Potential Chemical Delivery to Black-tailed Prairie Dogs (\u3ci\u3eCynomys ludovicianus\u3c/i\u3e)

Fertility control is a potential method to control prairie dog populations in the urban/suburban environment. However, an effective, oral delivery system is needed. We tested a food bait block delivery system that could make baits available to prairie dogs over a number of days which would make this method more cost-effective than placing food bait by hand near burrows every day. Prairie dogs readily consumed the bait blocks stacked on vertical metal poles during the day. We found, however, that rabbits and mice also consumed the food bait blocks, mainly at night. Over the course of the study, the mean amount removed per site was 81% of the food bait presented. However, to make the food bait blocks primarily available to prairie dogs, a device that would eliminate access to the food bait blocks at night is needed

The effects of vitamin K1-rich plant foods on the efficacy of the anticoagulant rodenticides chlorophacinone and diphacinone, used against Montane Voles (\u3ci\u3eMicrotus montanus\u3c/i\u3e)

Voles can cause significant losses to agriculture and wood fibre production. California growers typically rely on baits containing chlorophacinone and diphacinone to reduce vole population densities, but the efficacy of those rodenticides has been decreasing. One hypothesis suggests that voles are consuming high levels of an antidote (vitamin K1) to the anticoagulants, contained within green leafy plants. We tested that hypothesis by first feeding Montane Voles (Microtus montanus) diets that were high in vitamin K1, and then providing the animals with either: (1) chlorophacinone-containing bait, (2) diphacinone-containing bait, or (3) a control diet. We found that the chlorophacinone-containing bait remained efficacious (100% mortality), whereas the diphacinone-containing bait had a much lower efficacy (60% mortality). When only the diphacinone-containing bait was presented, the efficacy was somewhat better (80%). We infer that a diet rich in vitamin K1 did not negate the effects of the chlorophacinone for voles, and so we recommend its continued use in California unless anticoagulant resistance is known to have developed in the vole population. We hypothesise that: (1) diphacinone has a relatively low efficacy against Montane Voles when compared to chlorophacinone, and (2) this lower efficacy could be further reduced by a vitamin K1-rich diet

Design of a Self-Resetting, Low-Maintenance, Long-Term Bait Station for Rodent Control

A low-maintenance, long-term bait station that resets itself after being triggered would be a very useful tool for controlling Richardson’s ground squirrels, or other problem rodent species, in remote locations. With collaborators, we developed and tested two such devices using lab rats in pen settings. The devices can be left in-situ for long periods of time without servicing, and requires only occasional bait and/or battery replacement. Squirrels would be unable to cache bait due to the integrated time-out mechanism. The devices use capacitive sensor or strain gauge systems for animal identification, making it very unlikely that smaller non-target species would be able to trigger the systems while the design precludes entry by larger non-target species. Further refinement and testing will be needed before a viable, commercial product can go into production. These refinements include increasing reliability, reducing power requirements, design features and triggering mechanisms tightly linked to the attributes of the targeted pest species, and reduction of production costs. The devices will also need to be tested in field settings for extended periods of time

Compromised Fertility In Free Feeding Of Wildcaught Norway Rats (\u3ci\u3eRattus norvegicus\u3c/i\u3e) With A Liquid Bait Containing 4-Vinylcyclohexene Diepoxide And Triptolide

Wild rat pests in the environment cause crop and property damage and carry disease. Traditional methods of reducing populations of these pests involve poisons that can cause accidental exposures in other animals and humans. Fertility management with nonlethal chemicals would be an improved method of rat pest population control. Two chemicals known to target ovarian function in female rats are 4-vinylcyclohexene diepoxide (VCD) and triptolide. Additionally, triptolide impairs spermatogenesis in males. A liquid bait containing no active ingredients (control), or containing triptolide (0.001%) and VCD (0.109%; active) was prepared to investigate the potential use of these agents for wild rat pest population control. Liquid bait was made available to male (n = 8 control; n = 8 active) and female (n = 8 control; n = 8 active) Sprague Dawley rats (Rattus norvegicus) for oral consumption prior to breeding. Whereas, control bait-treated females produced normal-sized litters (10.0 ± 1.7 pups/litter), treated females delivered no pups. Wild Norway male (n = 20) and female (n = 20) rats (Rattus norvegicus) were trapped, individually housed, and one group given free access to control bait, one group to active bait. Following three cycles of treatment-matched mating pairs, females consuming control bait (control) produced normal litter sizes (9.73 ± 0.73 pups/litter). Females who had consumed active bait (treated) produced no litters on breeding cycles one and two; however, 2 of 10 females produced small litters on the third mating cycle. In a fourth breeding cycle, control females were crossmated with treated males, and treated females were crossmated with control males. In both groups, some dams produced litters, while others did not. The differences in response reflect a heterogeneity in return to cyclicity between females. These results suggest a potential approach to integrated pest management by compromising fertility, and could provide a novel alternative to traditional poisons for reducing populations of wild rat pests

Independent and combined effects of improved water, sanitation, and hygiene, and improved complementary feeding, on child stunting and anaemia in rural Zimbabwe: a cluster-randomised trial.

BACKGROUND: Child stunting reduces survival and impairs neurodevelopment. We tested the independent and combined effects of improved water, sanitation, and hygiene (WASH), and improved infant and young child feeding (IYCF) on stunting and anaemia in in Zimbabwe. METHODS: We did a cluster-randomised, community-based, 2 × 2 factorial trial in two rural districts in Zimbabwe. Clusters were defined as the catchment area of between one and four village health workers employed by the Zimbabwe Ministry of Health and Child Care. Women were eligible for inclusion if they permanently lived in clusters and were confirmed pregnant. Clusters were randomly assigned (1:1:1:1) to standard of care (52 clusters), IYCF (20 g of a small-quantity lipid-based nutrient supplement per day from age 6 to 18 months plus complementary feeding counselling; 53 clusters), WASH (construction of a ventilated improved pit latrine, provision of two handwashing stations, liquid soap, chlorine, and play space plus hygiene counselling; 53 clusters), or IYCF plus WASH (53 clusters). A constrained randomisation technique was used to achieve balance across the groups for 14 variables related to geography, demography, water access, and community-level sanitation coverage. Masking of participants and fieldworkers was not possible. The primary outcomes were infant length-for-age Z score and haemoglobin concentrations at 18 months of age among children born to mothers who were HIV negative during pregnancy. These outcomes were analysed in the intention-to-treat population. We estimated the effects of the interventions by comparing the two IYCF groups with the two non-IYCF groups and the two WASH groups with the two non-WASH groups, except for outcomes that had an important statistical interaction between the interventions. This trial is registered with ClinicalTrials.gov, number NCT01824940. FINDINGS: Between Nov 22, 2012, and March 27, 2015, 5280 pregnant women were enrolled from 211 clusters. 3686 children born to HIV-negative mothers were assessed at age 18 months (884 in the standard of care group from 52 clusters, 893 in the IYCF group from 53 clusters, 918 in the WASH group from 53 clusters, and 991 in the IYCF plus WASH group from 51 clusters). In the IYCF intervention groups, the mean length-for-age Z score was 0·16 (95% CI 0·08-0·23) higher and the mean haemoglobin concentration was 2·03 g/L (1·28-2·79) higher than those in the non-IYCF intervention groups. The IYCF intervention reduced the number of stunted children from 620 (35%) of 1792 to 514 (27%) of 1879, and the number of children with anaemia from 245 (13·9%) of 1759 to 193 (10·5%) of 1845. The WASH intervention had no effect on either primary outcome. Neither intervention reduced the prevalence of diarrhoea at 12 or 18 months. No trial-related serious adverse events, and only three trial-related adverse events, were reported. INTERPRETATION: Household-level elementary WASH interventions implemented in rural areas in low-income countries are unlikely to reduce stunting or anaemia and might not reduce diarrhoea. Implementation of these WASH interventions in combination with IYCF interventions is unlikely to reduce stunting or anaemia more than implementation of IYCF alone. FUNDING: Bill & Melinda Gates Foundation, UK Department for International Development, Wellcome Trust, Swiss Development Cooperation, UNICEF, and US National Institutes of Health.The SHINE trial is funded by the Bill & Melinda Gates Foundation (OPP1021542 and OPP113707); UK Department for International Development; Wellcome Trust, UK (093768/Z/10/Z, 108065/Z/15/Z and 203905/Z/16/Z); Swiss Agency for Development and Cooperation; US National Institutes of Health (2R01HD060338-06); and UNICEF (PCA-2017-0002)

Time allocation to resources by three species of rats (Rattus spp.) in a radial arm maze

Context. Introduced rats (Rattus spp.) can pose a serious threat to native flora and fauna, especially on islands where most species have evolved in the absence of terrestrial predators. Effective detection and eradication methods for introduced rats are essential to the maintenance of insular ecosystem integrity. Thus, it is important to better understand the behaviour of rats when they first arrive in a new setting. Aims. To determine whether rats would find some novel stimuli to be significantly more attractive than other novel stimuli. Methods. An eight-arm radial maze was used to study the behaviour of three species of Rattus finding themselves in a novel environment with various familiar and unfamiliar stimuli. Key results. Although there were some differences in responses by species and by sex, most rats sought out and spent considerable time in the den box, suggesting an immediate need for security when in an unfamiliar setting. Rats also sought out faeces of conspecifics, suggesting the need for social contact or reproduction. The rats, which had not been food deprived, did not seem interested in food sources, although there was some attraction to the water source. Implications. The management implications of the present study’s results are two-fold. First, appears that detection of newly arriving rats on islands would be aided by strategic placement of den boxes that are highly acceptable to rats. Managers could then inspect the den boxes periodically (or use a remote sensing system) for evidence of rat presence. Second, the den boxes could be scented with the faeces of other rats to further attract invading rats to the den boxes. This protocol might also hold the rats near the invasion site for a longer period of time before they begin seeking other shelter, food sources or mates. These protocols could give managers increased opportunities to detect any newly invading rats, and potentially increase the available time to deploy a rapid response to the invasion, before the animals begin to widely disperse. Of course, the rats will ultimately seek a source of palatable food, so placing durable, yet palatable, rodenticide bait in the den boxes might further decrease the probability of the invaders establishing a self-sustaining population

Deer Mice (\u3ci\u3ePeromyscus\u3c/i\u3e spp.) Biology, Damage and Management: A Review

The deer mouse is the most widely distributed and abundant small mammal in North America. They use a wide array of habitats, are very adaptable, and have a high reproductive potential. They play a number of roles in ecosystems, but can cause damage to orchards, forests, agriculture crops, and rangelands primarily through seed and newly-emerged seedling consumption. They also cause damage similar to house mice when they occupy buildings or other structures. Deer mice are important components in disease transmission especially of hantaviruses and Lyme disease. Damage reduction methods generally involve lethal control to reduce numbers using rodenticides and traps. Improvements in control methods are needed, especially in some agricultural crop types

Improving Invasive House Mice Control and Eradication Strategies via More Effective Rodenticides

In many cases, the control or eradication of invasive house mice has been problematic using rodenticide products that are currently registered. Our investigations using 12 commercial formulations confirmed that premise. In contrast, 11 of 12 of those commercial formulations were effective against Norway rats. Hence, we investigated 7 new rodenticide formulations to identity more effective alternative rodenticides (different formulation and/or different active ingredients). Several of the new formulations of rodenticides and new active ingredients were found to be relatively efficacious (\u3e70% mortality) and may warrant further investigation as potential control methods for invasive house mice. Additionally, a 2-active ingredient rodenticide, none of which are currently registered in the U.S., showed promise as a new house mouse control tool, and these may have sorne advantages over currently registered invasive house mouse rodenticides. Field trials with some of these new formulations are recommended as a next step in the research and pesticide registration process

Voles Damage Seedlings, But Do Deer Mice and House Mice?

While it is known that voles will damage seedlings, we do not know the extent to which deer mice and house mice damage seedlings. Knowing this information can assist resource managers in better targeting problem species and implementing appropriate management actions. We planted and monitored ponderosa pine and narrow-leaf cottonwood seedlings in metal stock tanks occupied by deer mice or house mice to assess the potential for damage by these rodents. Both species damaged leaves and stems of cottonwood seedlings, but house mice did more damage. House mouse damage resulted in mortality of over half of the cottonwood seedlings, while deer mice caused a much lower level of seedling mortality. Only slight damage was done by either species to pine seedlings. Neither species damaged the roots of seedlings, despite the extensive burrowing by house mice. While voles are often considered to be the primary rodent species causing seedling damage, we have shown that deer mice, and especially house mice, could also cause substantial damage to deciduous seedlings. However, our work suggests that rodent control to prevent damage to conifer seedlings might not be warranted in general unless extenuating circumstances and the species causing the damage are identified to assist with targeting control methods more precisely