192 research outputs found
Demonstration of catch bonds between an integrin and its ligand
Binding of integrins to ligands provides anchorage and signals for the cell, making them prime candidates for mechanosensing molecules. How force regulates integrināligand dissociation is unclear. We used atomic force microscopy to measure the force-dependent lifetimes of single bonds between a fibronectin fragment and an integrin Ī±5Ī²1-Fc fusion protein or membrane Ī±5Ī²1. Force prolonged bond lifetimes in the 10ā30-pN range, a counterintuitive behavior called catch bonds. Changing cations from Ca2+/Mg2+ to Mg2+/EGTA and to Mn2+ caused longer lifetime in the same 10ā30-pN catch bond region. A truncated Ī±5Ī²1 construct containing the headpiece but not the legs formed longer-lived catch bonds that were not affected by cation changes at forces <30 pN. Binding of monoclonal antibodies that induce the active conformation of the integrin headpiece shifted catch bonds to a lower force range. Thus, catch bond formation appears to involve force-assisted activation of the headpiece but not integrin extension
The integrins of the urochordate Ciona intestinalis provide novel insights into the molecular evolution of the vertebrate integrin family
BACKGROUND: Integrins are a functionally significant family of metazoan cell surface adhesion receptors. The receptors are dimers composed of an alpha and a beta chain. Vertebrate genomes encode an expanded set of integrin alpha and beta chains in comparison with protostomes such as drosophila or the nematode worm. The publication of the genome of a basal chordate, Ciona intestinalis, provides a unique opportunity to gain further insight into how and when the expanded integrin supergene family found in vertebrates evolved. RESULTS: The Ciona genome encodes eleven Ī± and five Ī² chain genes that are highly homologous to their vertebrate homologues. Eight of the Ī± chains contain an A-domain that lacks the short alpha helical region present in the collagen-binding vertebrate alpha chains. Phylogenetic analyses indicate the eight A-domain containing Ī± chains cluster to form an ascidian-specific clade that is related to but, distinct from, the vertebrate A-domain clade. Two Ciona Ī± chains cluster in laminin-binding clade and the remaining chain clusters in the clade that binds the RGD tripeptide sequence. Of the five Ciona Ī² chains, three form an ascidian-specific clade, one clusters in the vertebrate Ī²1 clade and the remaining Ciona chain is the orthologue of the vertebrate Ī²4 chain. CONCLUSION: The Ciona repertoire of integrin genes provides new insight into the basic set of these receptors available at the beginning of vertebrate evolution. The ascidian and vertebrate Ī± chain A-domain clades originated from a common precursor but radiated separately in each lineage. It would appear that the acquisition of collagen binding capabilities occurred in the chordate lineage after the divergence of ascidians
Hubble Space Telescope Imaging of the Circumstellar Nebulosity of T Tauri
Short-exposure Planetary Camera images of T Tauri have been obtained using broadband filters spanning the wavelength range 0.55-0.80 Ī¼m. The optically visible star lies very close to an arc of reflection nebulosity. The arc's northern arm extends approximately 5" from the star, while its southwestern arm appears brighter and extends only 2". The arc shows an approximate symmetry along an axis toward the west-northwest, the direction of Hind's Nebula and the blueshifted molecular outflow. The morphology of the reflected light is similar to models of scattered light within an illuminated, axisymmetric outflow cavity in a circumbinary envelope, viewed ā 45Ā° from the outflow axis. However, our model images do not successfully account for the amount of limb brightening that is seen. No optical counterpart to the infrared companion is seen to a limiting magnitude of V = 19.6, which suggests A_V > 7 mag toward this source. There is no evidence for an optical tertiary, to a limiting ĪV = 5.1 mag fainter than the primary, at the position where such an object has been previously reported
Stellar Populations at the Center of IC 1613
We have observed the center of the Local Group dwarf irregular galaxy IC 1613
with WFPC2 aboard the Hubble Space Telescope in the F439W, F555W, and F814W
filters. We find a dominant old stellar population (aged ~7 Gyr), identifiable
by the strong red giant branch (RGB) and red clump populations. From the (V-I)
color of the RGB, we estimate a mean metallicity of the intermediate-age
stellar population [Fe/H] = -1.38 +/- 0.31. We confirm a distance of 715 +/- 40
kpc using the I-magnitude of the RGB tip. The main-sequence luminosity function
down to I ~25 provides evidence for a roughly constant SFR of approximately
0.00035 solar masses per year across the WFPC2 field of view (0.22 square kpc)
during the past 250-350 Myr. Structure in the blue loop luminosity function
implies that the SFR was ~50% higher 400-900 Myr ago than today. The mean heavy
element abundance of these young stars is 1/10th solar. The best explanation
for a red spur on the main-sequence at I = 24.7 is the blue horizontal branch
component of a very old stellar population at the center of IC 1613. We have
also imaged a broader area of IC 1613 using the 3.5-meter WIYN telescope under
excellent seeing conditions. The AGB-star luminosity function is consistent
with a period of continuous star formation over at least the age range 2-10
Gyr. We present an approximate age-metallicity relation for IC 1613, which
appears similar to that of the Small Magellanic Cloud. We compare the Hess
diagram of IC 1613 to similar data for three other Local Group dwarf galaxies,
and find that it most closely resembles the nearby, transition-type dwarf
galaxy Pegasus (DDO 216).Comment: To appear in the September 1999 Astronomical Journal. LaTeX, uses
AASTeX v4.0, emulateapj style file, 19 pages, 12 postscript figures, 2
tables. 5 of the figures available separately via the WW
Hubble Space Telescope Observations of the Draco Dwarf Spheroidal
We present an F606W-F814W color-magnitude diagram for the Draco dwarf
spheroidal galaxy based on Hubble Space Telescope WFPC2 images. The luminosity
function is well-sampled to 3 magnitudes below the turn-off. We see no evidence
for multiple turnoffs and conclude that, at least over the field of the view of
the WFPC2, star formation was primarily single-epoch. If the observed number of
blue stragglers is due to extended star formation, then roughly 6% (upper
limit) of the stars could be half as old as the bulk of the galaxy. The color
difference between the red giant branch and the turnoff is consistent with an
old population and is very similar to that observed in the old, metal-poor
Galactic globular clusters M68 and M92. Despite its red horizontal branch,
Draco appears to be older than M68 and M92 by 1.6 +/- 2.5 Gyrs, lending support
to the argument that the ``second parameter'' which governs horizontal branch
morphology must be something other than age. Draco's observed luminosity
function is very similar to that of M68, and the derived initial mass function
is consistent with that of the solar neighborhood.Comment: 16 pages, AASTeX, 9 postscript figures, figures 1 and 2 available at
ftp://bb3.jpl.nasa.gov/pub/draco/. Accepted for publication in the
Astronomical Journa
Observations and Implications of the Star Formation History of the LMC
We present derivations of star formation histories based on color-magnitude
diagrams of three fields in the LMC from HST/WFPC2 observations. A significant
component of stars older than 4 Gyr is required to match the observed
color-magnitude diagrams. Models with a dispersion-free age-metallicity
relation are unable to reproduce the width of the observed main sequence;
models with a range of metallicity at a given age provide a much better fit.
Such models allow us to construct complete ``population boxes'' for the LMC
based entirely on color-magnitude diagrams; remarkably, these qualitatively
reproduce the age-metallicity relation observed in LMC clusters. We discuss
some of the uncertainties in deriving star formation histories. We find,
independently of the models, that the LMC bar field has a larger relative
component of older stars than the outer fields. The main implications suggested
by this study are: 1) the star formation history of field stars appears to
differ from the age distribution of clusters, 2) there is no obvious evidence
for bursty star formation, but our ability to measure bursts shorter in
duration than 25% of any given age is limited by the statistics of the
observed number of stars, 3) there may be some correlation of the star
formation rate with the last close passage of the LMC/SMC/Milky Way, but there
is no dramatic effect, and 4) the derived star formation history is probably
consistent with observed abundances, based on recent chemical evolution models.Comment: Accepted by AJ, 36 pages including 12 figure
Stellar Populations in Three Outer Fields of the LMC
We present HST photometry for three fields in the outer disk of the LMC
extending approximately four magnitudes below the faintest main sequence
turnoff. We cannot detect any strongly significant differences in the stellar
populations of the three fields based on the morphologies of the
color-magnitude diagrams, the luminosity functions, and the relative numbers of
stars in different evolutionary stages. Our observations therefore suggest
similar star formation histories in these regions, although some variations are
certainly allowed. The fields are located in two regions of the LMC: one is in
the north-east field and two are located in the north-west. Under the
assumption of a common star formation history, we combine the three fields with
ground-based data at the same location as one of the fields to improve
statistics for the brightest stars. We compare this stellar population with
those predicted from several simple star formation histories suggested in the
literature, using a combination of the R-method of Bertelli et al (1992) and
comparisons with the observed luminosity function. The only model which we
consider that is not rejected by the observations is one in which the star
formation rate is roughly constant for most of the LMC's history and then
increases by a factor of three about 2 Gyr ago. Such a model has roughly equal
numbers of stars older and younger than 4 Gyr, and thus is not dominated by
young stars. This star formation history, combined with a closed box chemical
evolution model, is consistent with observations that the metallicity of the
LMC has doubled in the past 2 Gyr.Comment: 30 pages, includes 10 postscript figures. Figure 1 avaiable at
ftp://charon.nmsu.edu/pub/mgeha/LMC. Accepted for publication in Astronomical
Journa
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