Les missions de la police judiciaire en matière de droit alimentaire au Maroc. Etude descriptive

Parce qu’elles représentent aujourd’hui au Maroc un intérêt grandissant pour les pouvoirs publics et les consommateurs, les missions de la police judiciaire des agents habilités en droit alimentaire méritent réflexion. Incontestablement, le respect des textes juridiques alimentaires est subordonné à l’existence de sanctions dissuasives mais aussi à des actions efficaces de la police judicaire des autorités compétentes en matière alimentaire. A ce titre, les agents habilités à contrôler doivent avoir une maitrise parfaite des règles de procédures liées à la police judiciaire, doivent aussi connaitre toutes les infractions pénales alimentaires en vigueur et leurs sanctions et enfin doivent comprendre les enjeux actuels et futurs de la police judiciaire en droit alimentaire marocain. C’est dans la visée de ces objectifs que notre étude a été menée. Elle passera en revue les principales exigences des textes du droit pénal alimentaire marocain, apportera quelques analyses, directives et recommandations aux agents habilités à assurer le contrôle et à l’autorité compétente en charge de la répression

Beekeeping in Morocco: focus on honey production

Beekeeping is an agricultural sector that has a special character in Morocco. It is virtually present in different regions. The apiarian potentialities in Morocco are important and remain under exploited. Honey production is stagnating because of many difficulties concerning repopulating colonies despite governmental efforts to modernize the sector. This review is to present a synthesis about the sector of beekeeping and honey production in Morocco. It will allow describing the situations, to understand the challenges faced by the Moroccan beekeeping by focusing on: bee farming, honey production, professional organization, marketing and regulatory issues. The main issues of this sector is the preservation of the Saharan yellow bee, increasing honey production and productivity of the hives, the application of good hygiene practices, enhancing honey quality, regulatory revision, adjusting the current model of professional organization, structuring of honey marketing channels and finally conclude with recommendations and prospects.L’apiculture est une filière agricole qui a un caractère spécial au Maroc. Elle est pratiquement présente dans les différentes régions du Royaume. Les potentialités apicoles au Maroc sont importantes et pourtant, aujourd'hui, la filière apicole reste sous exploitée avec une production de miel qui stagne à cause de beaucoup de difficultés à repeupler les colonies malgré les efforts de l’Etat en vue de la modernisation de la filière apicole. L’objectif de ce travail de revue et de synthèse est de faire un état des lieux de la filière apicole, en l’occurrence la production du miel en nous appuyant sur le peu de données bibliographiques disponibles. Cet article permettra de mieux appréhender les enjeux auxquelles fait face la filière apicole marocaine. Il sera focalisé sur : l’élevage apicole, la production de miel, l’organisation professionnelle, la commercialisation et aux aspects réglementaires. Les principaux enjeux de cette filière sont la préservation de la race l’abeille jaune saharienne, l’augmentation de la production du miel et de la productivité des ruches, l’application des bonnes pratiques sanitaires apicoles, le renforcement de la qualité du miel, la révision du cadre réglementaire, l’ajustement du modèle d’organisation professionnelle actuelle et la structuration des circuits de commercialisation du miel. Enfin, une perspective a été tracée

Microbiological quality of Moroccan labeled Euphorbia resinifera honey

In the present work, microbiological profile of thirty-seven samples of labeled honey were collected in a Protected Geographical Indication “PGI” area of Tadla-Azilal region, which is an endemic zone of Euphorbia resinifera plant. A profile was assessed using conventional microbial methods, like enumeration, detection and/or germs identification, in accordance with ISO norms. This is the first study in which a honey with Moroccan “PGI” was tested, in order to assess its compliance with bacteriological recommendations. Coliforms (Total and fecal Coliforms), Salmonella spp., Shigella spp., Sporus of Bacillus cereus and Clostridium perfringens were not detected. The numbers of Standard Plate Count “SPC” were less than 102 CFU.g-1 for all samples. The molds and yeasts were found among samples and 32% and 40% of samples were positive, respectively. However, no samples showed a higher value than recommended limit [102 CFU.g-1]. We conclude that samples of labeled euphorbia honey of Tadla-Azilal analyzed present good commercial quality parameters (SPC, molds and yeasts “absence of unwanted fermentations”), a good sanitary quality (absence of coliforms and S. aureus) and are safe (Slam., Shig., Sporus of B. cereus and C. perf.). Standardization (regulation and specifications) and a rationalization of beekeeping techniques throughout Euphorbia “PGI” area studied may further sustainably improve the quality of this unique honey, and ensure it over the years

Relationship among antibiotic residues and antibacterial activity of the endemic spurge honey (Euphorbia Resinifera o. Berg) from morocco

Antibiotic-resistant bacteria continue to be of major health concern worldwide. In recent years, several reports and scientific articles claim the contamination of honey by antibiotics, detectable concentrations of antibiotic residues in honey are illegal. They, may cause hypersensitivity or resistance to drug therapy in humans, and are perceived by consumers as undesirable. In this sense, the purpose of this work was to examine the antibacterial activity of the Euphorbia resinifera (E. resinifera) honey against Escherichia coli and Staphylococcus aureus in vitro using the well-agar diffusion assay followed by dilution range to obtain more precise minimum inhibitory concentration values. The second aim is to evaluate the presence of antibiotics in honey using a screening test: Evidence Investigator™, an immuno-enzymatic method for detection of 27 antibiotic residues followed by a liquid chromatography-tandem mass spectrometry (LC-MS/MS) for confirmation of suspect samples; in order to assess the relationship between the presence of antibiotic residues and the antibacterial activity of honey. In this study, a total of 37 E. resinifera honey samples were analyzed. The results show that all samples of honey inhibited the growth of bacteria at the dilutions at 50% (v/v); the highest inhibition zone (25.98 ± 0.11 mm) was recorded from sample 5 for Staphylococcus aureus and (13.84 ± 1.10 mm) in sample 17 for Escherichia coli and that 50% (v/v) dilutions showed significant antibacterial effect compared to other dilutions (6.25, 12.5, 25% (v/v)). In all samples, there were no antibiotic residues detected except for one showing the detection of Trimethoprim at 6.48 µg kg-1. Our research is one of the first studies that relate the he relationship between the presence of antibiotic residues and the antibacterial activity of Euphorbia resinifera honey and showed that the antibacterial activity of honey might be due to the high osmotic nature, a low pH, its content of phenolic compounds and hydrogen peroxide and also to its content of methylglyoxal

Phenolic compounds and color of labeled resin spurge honey and their correlations with pollen content

Our aim in this study was to outline phenolic and color characteristic that characterize the labeled unifloral resin spurge (Euphorbia resinifera) honey. With respect to phenolic composition, 17 phenolic compounds have been analyzed in the 29 honey samples. The proposed markers (syringic acid, ethyl gallate, m-coumaric acid and naringenin) might help to the enhancement of this honey type and thus, guarantee its commercial value. The color characterization by diffuse reflectance spectrophotometry revealed typical values of light amber honey (lightness ranged from 36 to 70 units, and chroma from 18 to 30 units). On the other hand, many correlations between the color attributes and phenolic acids, total phenolic compounds, caffeic acid, p- and m-coumaric acids and hesperidin have been demonstrated, also, correlation between phenols, color parameters and percentage of pollen of E. resinifera has been found. This study is one of the rare researches which have correlated the CIELAB color parameters with the individual phenolic acids and flavonoid compounds within the same unifloral honey

Comparative evaluation of antioxidant activity, total phenolic content, anti-inflammatory, and antibacterial potential of Euphorbia-derived functional products

This study assessed the medicinal properties of Euphorbia resinifera O. Berg (E. resinifera) and Euphorbia officinarum subsp echinus (Hook.f. and Coss.) Vindt (Euphorbia echinus, known for their pharmaceutical benefits. Extracts from their flowers, stems, propolis, and honey were examined for phenolic content, antioxidant, anti-inflammatory, and antibacterial activities. Total phenolic content (TPC), total flavonoid content (TFC), and total condensed tannin (TCC) were determined using specific methods. Antioxidant potential was assessed through various tests including DPPH, FRAP, ABTS, and Total antioxidant capacity. Anti-inflammatory effects were evaluated using phenol-induced ear edema in rats, while antibacterial activity was measured against Gram-positive (Staphylococcus aureus ATCC 6538) and Gram-negative (E. coli ATCC 10536) bacteria. Among the extracts, the aqueous propolis extract of E. resinifera demonstrated exceptional antioxidant capabilities, with low IC50 values for DPPH (0.07 ± 0.00 mg/mL) and ABTS (0.13 ± 0.00 mg/mL), as well as high TAC (176.72 ± 0.18 mg AA/mg extract) and FRAP (86.45 ± 1.45 mg AA/mg extract) values. Furthermore, the anti-inflammatory effect of E. resinifera propolis extracts surpassed that of indomethacin, yielding edema percentages of 3.92% and 11.33% for the aqueous and ethanolic extracts, respectively. Microbiological results indicated that the aqueous extract of E. resinifera flower exhibited the most potent inhibitory action against S. aureus, with an inhibition zone diameter (IZD) of 21.0 ± 0.00 mm and a minimum inhibitory concentration (MIC) of 3.125 mg/mL. Additionally, only E. resinifera honey displayed the ability to inhibit E. coli growth, with an inhibition zone diameter of 09.30 ± 0.03 mm and a MIC of 0.0433 mg/mL

Table_2_Euphorbia species latex: A comprehensive review on phytochemistry and biological activities.docx

The genus Euphorbia includes about 2,000 species commonly widespread in both temperate and tropical zones that contain poisonous milky juice fluid or latex. Many species have been used in traditional and complementary medicine for the treatment of various health issues such as dropsy, paralysis, deafness, wounds, warts on the skin, and amaurosis. The medicinal applications of these species have been attributed to the presence of various compounds, and most studies on Euphorbia species have focused on their latex. In this review, we summarize the current state of knowledge on chemical composition and biological activities of the latex from various species of the genus Euphorbia. Our aim was to explore the applications of latex extracts in the medical field and to evaluate their ethnopharmacological potential. The databases employed for data collection, are obtained through Web of Science, PubMed, Google Scholar, Science Direct and Scopus, from 1983 to 2022. The bibliographic data indicate that terpenoids are the most common secondary metabolites in the latex. Furthermore, the latex has interesting biological properties and pharmacological functions, including antibacterial, antioxidant, free radical scavenger, cytotoxic, tumor, anti-inflammatory, healing, hemostatic, anti-angiogenic, insecticidal, genotoxic, and mutagenic activities. However, the role of other components in the latex, such as phenolic compounds, alkaloids, saponins, and flavonoids, remains unknown, which limits the application of the latex. Future studies are required to optimize the therapeutic use of latex extracts.</p

Table1_Comparative evaluation of antioxidant activity, total phenolic content, anti-inflammatory, and antibacterial potential of Euphorbia-derived functional products.docx

This study assessed the medicinal properties of Euphorbia resinifera O. Berg (E. resinifera) and Euphorbia officinarum subsp echinus (Hook.f. and Coss.) Vindt (Euphorbia echinus, known for their pharmaceutical benefits. Extracts from their flowers, stems, propolis, and honey were examined for phenolic content, antioxidant, anti-inflammatory, and antibacterial activities. Total phenolic content (TPC), total flavonoid content (TFC), and total condensed tannin (TCC) were determined using specific methods. Antioxidant potential was assessed through various tests including DPPH, FRAP, ABTS, and Total antioxidant capacity. Anti-inflammatory effects were evaluated using phenol-induced ear edema in rats, while antibacterial activity was measured against Gram-positive (Staphylococcus aureus ATCC 6538) and Gram-negative (E. coli ATCC 10536) bacteria. Among the extracts, the aqueous propolis extract of E. resinifera demonstrated exceptional antioxidant capabilities, with low IC50 values for DPPH (0.07 ± 0.00 mg/mL) and ABTS (0.13 ± 0.00 mg/mL), as well as high TAC (176.72 ± 0.18 mg AA/mg extract) and FRAP (86.45 ± 1.45 mg AA/mg extract) values. Furthermore, the anti-inflammatory effect of E. resinifera propolis extracts surpassed that of indomethacin, yielding edema percentages of 3.92% and 11.33% for the aqueous and ethanolic extracts, respectively. Microbiological results indicated that the aqueous extract of E. resinifera flower exhibited the most potent inhibitory action against S. aureus, with an inhibition zone diameter (IZD) of 21.0 ± 0.00 mm and a minimum inhibitory concentration (MIC) of 3.125 mg/mL. Additionally, only E. resinifera honey displayed the ability to inhibit E. coli growth, with an inhibition zone diameter of 09.30 ± 0.03 mm and a MIC of 0.0433 mg/mL.</p