A Python based automated tracking routine for myosin II filaments

The study of motor protein dynamics within cytoskeletal networks is of high interest to physicists and biologists to understand how the dynamics and properties of individual motors lead to cooperative effects and control of overall network behaviour. Here, we report a method to detect and track muscular myosin II filaments within an actin network tethered to supported lipid bilayers. Based on the characteristic shape of myosin II filaments, this automated tracking routine allowed us to follow the position and orientation of myosin II filaments over time, and to reliably classify their dynamics into segments of diffusive and processive motion based on the analysis of displacements and angular changes between time steps. This automated, high throughput method will allow scientists to efficiently analyse motor dynamics in different conditions, and will grant access to more detailed information than provided by common tracking methods, without any need for time consuming manual tracking or generation of kymographs

Myosin II filament dynamics in actin networks revealed with interferometric scattering microscopy

The plasma membrane and the underlying cytoskeletal cortex constitute active platforms for a variety of cellular processes. Recent work has shown that the remodeling acto-myosin network modifies local membrane organization, but the molecular details are only partly understood due to difficulties with experimentally accessing the relevant time and length scales. Here, we use interferometric scattering (iSCAT) microscopy to investigate a minimal acto-myosin network linked to a supported lipid bilayer membrane. Using the magnitude of the interferometric contrast, which is proportional to molecular mass, and fast acquisition rates, we detect, and image individual membrane attached actin filaments diffusing within the acto-myosin network and follow individual myosin II filament dynamics. We quantify myosin II filament dwell times and processivity as functions of ATP concentration, providing experimental evidence for the predicted ensemble behavior of myosin head domains. Our results show how decreasing ATP concentrations lead to both increasing dwell times of individual myosin II filaments and a global change from a remodeling to a contractile state of the acto-myosin network

First Observation of 15Be

The neutron-unbound nucleus 15Be was observed for the first time. It was populated using neutron transfer from a deuterated polyethylene target with a 59 MeV/u 14Be beam. Neutrons were measured in coincidence with outgoing 14Be particles and the reconstructed decay energy spectrum exhibits a resonance at 1.8(1) MeV. This corresponds to 15Be being unbound by 0.45 MeV more then 16Be thus significantly hindering the sequential two-neutron decay of 16Be to 14Be through this state

Spectroscopy of neutron-unbound 27,28^{27,28}F

The ground state of $^{28}$F has been observed as an unbound resonance $2\underline{2}0$ keV above the ground state of $^{27}$F. Comparison of this result with USDA/USDB shell model predictions leads to the conclusion that the $^{28}$F ground state is primarily dominated by $sd$-shell configurations. Here we present a detailed report on the experiment in which the ground state resonance of $^{28}$F was first observed. Additionally, we report the first observation of a neutron-unbound excited state in $^{27}$F at an excitation energy of $25\underline{0}0 (2\underline{2}0)$ keV.Comment: 10 pages, 11 figures, Accepted for publication in Phys. Rev.

Observation of a low-lying neutron-unbound state in 19C

Proton removal reactions from a secondary 22N beam were utilized to populate unbound states in neutron-rich carbon isotopes. Neutrons were measured with the Modular Neutron Array (MoNA) in coincidence with carbon fragments. A resonance with a decay energy of 76(14) keV was observed in the system 18C+n corresponding to a state in 19C at an excitation energy of 653(95)keV. This resonance could correspond to the first 5/2+ state which was recently speculated to be unbound in order to describe 1n and 2n removal cross section measurements from 20C.Comment: accepted for publication in Nucl. Phys.

Exploring the Low-ZZ Shore of the Island of Inversion at N=19N = 19

The technique of invariant mass spectroscopy has been used to measure, for the first time, the ground state energy of neutron-unbound $^{28}\textrm{F},$ determined to be a resonance in the $^{27}\textrm{F} + n$ continuum at $2\underline{2}0 (\underline{5}0)$ keV. States in $^{28}\textrm{F}$ were populated by the reactions of a 62 MeV/u $^{29}\textrm{Ne}$ beam impinging on a 288 $\textrm{mg/cm}^2$ beryllium target. The measured $^{28}\textrm{F}$ ground state energy is in good agreement with USDA/USDB shell model predictions, indicating that $pf$ shell intruder configurations play only a small role in the ground state structure of $^{28}\textrm{F}$ and establishing a low-$Z$ boundary of the island of inversion for N=19 isotones.Comment: 5 pages, 4 figures, to be published in Phys. Rev. Let

Three-body correlations in the ground-state decay of 26O

Background: Theoretical calculations have shown that the energy and angular correlations in the three-body decay of the two-neutron unbound O26 can provide information on the ground-state wave function, which has been predicted to have a dineutron configuration and 2n halo structure. Purpose: To use the experimentally measured three-body correlations to gain insight into the properties of O26, including the decay mechanism and ground-state resonance energy. Method: O26 was produced in a one-proton knockout reaction from F27 and the O24+n+n decay products were measured using the MoNA-Sweeper setup. The three-body correlations from the O26 ground-state resonance decay were extracted. The experimental results were compared to Monte Carlo simulations in which the resonance energy and decay mechanism were varied. Results: The measured three-body correlations were well reproduced by the Monte Carlo simulations but were not sensitive to the decay mechanism due to the experimental resolutions. However, the three-body correlations were found to be sensitive to the resonance energy of O26. A 1{\sigma} upper limit of 53 keV was extracted for the ground-state resonance energy of O26. Conclusions: Future attempts to measure the three-body correlations from the ground-state decay of O26 will be very challenging due to the need for a precise measurement of the O24 momentum at the reaction point in the target

Search for unbound 15Be states in the 3n+12Be channel

15Be is expected to have low-lying 3/2+ and 5/2+ states. A first search did not observe the 3/2+ [A. Spyrou et al., Phys. Rev. C 84, 044309 (2011)], however, a resonance in 15Be was populated in a second attempt and determined to be unbound with respect to 14Be by 1.8(1) MeV with a tentative spin-parity assignment of 5/2+ [J. Snyder et al., Phys. Rev. C 88, 031303(R) (2013)]. Search for the predicted 15Be 3/2+ state in the three-neutron decay channel. A two-proton removal reaction from a 55 MeV/u 17C beam was used to populate neutron-unbound states in 15Be. The two-, three-, and four-body decay energies of the 12Be + neutron(s) detected in coincidence were reconstructed using invariant mass spectroscopy. Monte Carlo simulations were performed to extract the resonance and decay properties from the observed spectra. The low-energy regions of the decay energy spectra can be described with the first excited unbound state of 14Be (E_x=1.54 MeV, E_r=0.28 MeV). Including a state in 15Be that decays through the first excited 14Be state slightly improves the fit at higher energies though the cross section is small. A 15Be component is not needed to describe the data. If the 3/2+ state in 15Be is populated, the decay by three-neutron emission through 14Be is weak, less than or equal to 11% up to 4 MeV. In the best fit, 15Be is unbound with respect to 12Be by 1.4 MeV (unbound with respect to $14Be by 2.66 MeV) with a strength of 7%.Comment: 6 pages, 5 figures, accepted in Physical Review

Enhanced low-energy γ\gamma-decay strength of 70^{70}Ni and its robustness within the shell model

Neutron-capture reactions on very neutron-rich nuclei are essential for heavy-element nucleosynthesis through the rapid neutron-capture process, now shown to take place in neutron-star merger events. For these exotic nuclei, radiative neutron capture is extremely sensitive to their $\gamma$-emission probability at very low $\gamma$ energies. In this work, we present measurements of the $\gamma$-decay strength of $^{70}$Ni over the wide range $1.3 \leq E_{\gamma} \leq 8$ MeV. A significant enhancement is found in the $\gamma$-decay strength for transitions with $E_\gamma < 3$ MeV. At present, this is the most neutron-rich nucleus displaying this feature, proving that this phenomenon is not restricted to stable nuclei. We have performed $E1$-strength calculations within the quasiparticle time-blocking approximation, which describe our data above $E_\gamma \simeq 5$ MeV very well. Moreover, large-scale shell-model calculations indicate an $M1$ nature of the low-energy $\gamma$ strength. This turns out to be remarkably robust with respect to the choice of interaction, truncation and model space, and we predict its presence in the whole isotopic chain, in particular the neutron-rich $^{72,74,76}\mathrm{Ni}$.Comment: 9 pages, 9 figure