Take a Walk: A Critical Reflection on Data Gathering in Remote Island Communities

This paper is an account of a critical refection on the process of data gathering in remote island communities by phone as an insider-outsider. The purpose of the study was to contribute to my PhD research question: What factors influence successful contemporary migration of Torres Strait Islanders who are moving to the Australian mainland? To achieve this I had to contact remote Island communities in the Torres Strait and evolve a process that was relevant, reliable, and appropriate to Torres Strait Islander people and their communities. Semi-structured phone surveys where completed with key informants for each Island community. The process involved walking beside the participants on a virtual tour, house-by-house, and street-by-street. The process uncovered hidden nuances that surround accessing and retrieving information. The findings are useful, relevant and transferable for advancing research methods for collecting information in remote areas

A Python based automated tracking routine for myosin II filaments

The study of motor protein dynamics within cytoskeletal networks is of high interest to physicists and biologists to understand how the dynamics and properties of individual motors lead to cooperative effects and control of overall network behaviour. Here, we report a method to detect and track muscular myosin II filaments within an actin network tethered to supported lipid bilayers. Based on the characteristic shape of myosin II filaments, this automated tracking routine allowed us to follow the position and orientation of myosin II filaments over time, and to reliably classify their dynamics into segments of diffusive and processive motion based on the analysis of displacements and angular changes between time steps. This automated, high throughput method will allow scientists to efficiently analyse motor dynamics in different conditions, and will grant access to more detailed information than provided by common tracking methods, without any need for time consuming manual tracking or generation of kymographs

Myosin II filament dynamics in actin networks revealed with interferometric scattering microscopy

The plasma membrane and the underlying cytoskeletal cortex constitute active platforms for a variety of cellular processes. Recent work has shown that the remodeling acto-myosin network modifies local membrane organization, but the molecular details are only partly understood due to difficulties with experimentally accessing the relevant time and length scales. Here, we use interferometric scattering (iSCAT) microscopy to investigate a minimal acto-myosin network linked to a supported lipid bilayer membrane. Using the magnitude of the interferometric contrast, which is proportional to molecular mass, and fast acquisition rates, we detect, and image individual membrane attached actin filaments diffusing within the acto-myosin network and follow individual myosin II filament dynamics. We quantify myosin II filament dwell times and processivity as functions of ATP concentration, providing experimental evidence for the predicted ensemble behavior of myosin head domains. Our results show how decreasing ATP concentrations lead to both increasing dwell times of individual myosin II filaments and a global change from a remodeling to a contractile state of the acto-myosin network

Sociabilidades efímeras: la navegación social entre los jóvenes daneses

Aquest article analitza les maneres de reunir-se dels joves a les ciutats daneses d’Aarhus i Horsens, tenint en compte les complexitats espacials, temporals i socials de l’espai urbà. Suggerim el terme de «sociabilitats urbanes efímeres» per entendre com es construeix un estar junts alternatiu, fora de marcs institucionals o socials formals. Basant-nos en el treball de camp antropològic d’Anne-Lene Sand, ens centrem en un context en què el desenvolupament de l’espai urbà sembla haver disminuït les oportunitats perquè els joves definisquen i creen espais per a si mateixos. Aquest article se centra en les formes de reunir-se en un context que a primera vista sembla altament regulat i planejat, però que des d’una altra perspectiva és incert (Highmore, 2005; Lefebvre, 1994) i està obert a la reinterpretació lúdica (Stevens, 2007) . El material es discuteix a través del concepte de «navegació social» de l’antropòleg danés Henrik Vigh (2006, 2009), per entendre les formacions socials mòbils i canviants dels joves en el context urbà. Busquem contribuir al coneixement sobre les sociabilitats juvenils urbanes modernes que no poden ser descrites com grupals o territorials, però que en canvi es construeixen a través del desig de trobar-se amb persones afins.This article analyses how young people get together in the spatial, temporal and social complexity of urban space. We suggest the term ephemeral urban socialities to understand how young people construct alternative socialities that are not embedded within an institutional mode of thinking or a formalised social setting. Based on anthropological fieldwork and empirical material generated in the Danish cities of Aarhus and Horsens by Anne-Lene Sand, we frame the analysis in a context where the development of urban space minimises social places that young people can define by and for themselves. This article investigates how young people come together socially in a context that seems to be highly regulated and planned, but that from another perspective is uncertain (Highmore, 2005; Lefebvre, 1994) and open to ludic interpretation (Stevens, 2007). The material is discussed through the lens of the Danish anthropologist Henrik Vigh’s concept of social navigation (2006, 2009) to understand young people’s mobile and changing social formations in the urban context. This article contributes knowledge about modern urban socialities in medium-sized northern European cities that, in the case of youth formations, cannot be described as groups or as territorial, but that are constructed through the desire to meet with “like-minded individuals”.Este artículo analiza las maneras de reunirse de los jóvenes en lasB ciudades danesas de Aarhus y Horsens, teniendo en cuenta las complejidades espaciales, temporales y sociales del espacio urbano. Sugerimos el término de «sociabilidades urbanas efímeras» para entender cómo se construye un estar juntos alternativo, fuera de marcos institucionales o sociales formales. Basándonos en el trabajo de campo antropológico de Anne-Lene Sand, nos centramos en un contexto en el que el desarrollo del espacio urbano parece haber disminuido las oportunidades para que los jóvenes definan y creen espacios para sí mismos. Este artículo se centra en las formas de reunirse en un contexto que a simple vista parece altamente regulado y planeado, pero que desde otra perspectiva es incierto (Highmore, 2005; Lefebvre, 1994) y está abierto a la reinterpretación lúdica (Stevens, 2007). El material se discute a través del concepto de «navegación social» del antropólogo danés Henrik Vigh (2006, 2009), para entender las formaciones sociales móviles y cambiantes de los jóvenes en el contexto urbano. Buscamos contribuir al conocimiento sobre las sociabilidades juveniles urbanas modernas que no pueden ser descritas como grupales o territoriales, pero que en cambio se construyen a través del deseo de encontrarse con personas afines

Spectroscopy of neutron-unbound 27,28^{27,28}F

The ground state of $^{28}$F has been observed as an unbound resonance $2\underline{2}0$ keV above the ground state of $^{27}$F. Comparison of this result with USDA/USDB shell model predictions leads to the conclusion that the $^{28}$F ground state is primarily dominated by $sd$-shell configurations. Here we present a detailed report on the experiment in which the ground state resonance of $^{28}$F was first observed. Additionally, we report the first observation of a neutron-unbound excited state in $^{27}$F at an excitation energy of $25\underline{0}0 (2\underline{2}0)$ keV.Comment: 10 pages, 11 figures, Accepted for publication in Phys. Rev.

First Observation of 15Be

The neutron-unbound nucleus 15Be was observed for the first time. It was populated using neutron transfer from a deuterated polyethylene target with a 59 MeV/u 14Be beam. Neutrons were measured in coincidence with outgoing 14Be particles and the reconstructed decay energy spectrum exhibits a resonance at 1.8(1) MeV. This corresponds to 15Be being unbound by 0.45 MeV more then 16Be thus significantly hindering the sequential two-neutron decay of 16Be to 14Be through this state

Evaluation of the nutrition screening tool for childhood cancer (SCAN)

Background & aims: Malnutrition is a serious concern for children with cancer and nutrition screening may offer a simple alternative to nutrition assessment for identifying children with cancer who are at risk of malnutrition. The present paper aimed to evaluate the nutrition screening tool for childhood cancer (SCAN). Methods: SCAN was developed after an extensive review of currently available tools and published screening recommendation, consideration of pediatric oncology nutrition guidelines, piloting questions, and consulting with members of International Pediatric Oncology Nutrition Group. In Study 1, the accuracy and validity of SCAN against pediatric subjective global nutrition assessment (pediatric SGNA) was determined. In Study 2, subjects were classified as 'at risk of malnutrition' and 'not at risk of malnutrition' according to SCAN and measures of height, weight, body mass index (BMI) and body composition were compared between the groups. Results: The validation of SCAN against pediatric SGNA showed SCAN had 'excellent' accuracy (0.90, 95% CI 0.78-1.00; p < 0.001), 100% sensitivity, 39% specificity, 56% positive predictive value and 100% negative predictive value. When subjects in Study 2 were classified into 'at risk of malnutrition' and 'not at risk of malnutrition' according to SCAN, the 'at risk of malnutrition' group had significantly lower values for weight Z score (p = 0.001), BMI Z score (p = 0.001) and fat mass index (FMI) (p = 0.04), than the 'not at risk of malnutrition' group. Conclusions: This study shows that SCAN is a simple, quick and valid tool which can be used to identify children with cancer who are at risk of malnutrition