What agricultural practices are most likely to deliver ‘sustainable intensification’ in the UK?

Sustainable intensification is a process by which agricultural productivity is enhanced whilst also creating environmental and social benefits. We aimed to identify practices likely to deliver sustainable intensification, currently available for UK farms but not yet widely adopted. We compiled a list of 18 farm management practices with the greatest potential to deliver sustainable intensification in the UK, following a well-developed stepwise methodology for identifying priority solutions, using a group decision-making technique with key agricultural experts. The list of priority management practices can provide the focal point of efforts to achieve sustainable intensification of agriculture, as the UK develops post-Brexit agricultural policy, and pursues the second Sustainable Development Goal, which aims to end hunger and promote sustainable agriculture. The practices largely reflect a technological, production-focused view of sustainable intensification, including for example, precision farming and animal health diagnostics, with less emphasis on the social and environmental aspects of sustainability. However, they do reflect an integrated approach to farming, covering many different aspects, from business organization and planning, to soil and crop management, to landscape and nature conservation. For a subset of ten of the priority practices, we gathered data on the level of existing uptake in English and Welsh farms through a stratified survey in seven focal regions. We find substantial existing uptake of most of the priority practices, indicating that UK farming is an innovative sector. The data identify two specific practices for which uptake is relatively low, but which some UK farmers find appealing and would consider adopting. These practices are: prediction of pest and disease outbreaks, especially for livestock farms; staff training on environmental issues, especially on arable farms

SARS-CoV-2-specific immune responses and clinical outcomes after COVID-19 vaccination in patients with immune-suppressive disease

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immune responses and infection outcomes were evaluated in 2,686 patients with varying immune-suppressive disease states after administration of two Coronavirus Disease 2019 (COVID-19) vaccines. Overall, 255 of 2,204 (12%) patients failed to develop anti-spike antibodies, with an additional 600 of 2,204 (27%) patients generating low levels (<380 AU ml−1). Vaccine failure rates were highest in ANCA-associated vasculitis on rituximab (21/29, 72%), hemodialysis on immunosuppressive therapy (6/30, 20%) and solid organ transplant recipients (20/81, 25% and 141/458, 31%). SARS-CoV-2-specific T cell responses were detected in 513 of 580 (88%) patients, with lower T cell magnitude or proportion in hemodialysis, allogeneic hematopoietic stem cell transplantation and liver transplant recipients (versus healthy controls). Humoral responses against Omicron (BA.1) were reduced, although cross-reactive T cell responses were sustained in all participants for whom these data were available. BNT162b2 was associated with higher antibody but lower cellular responses compared to ChAdOx1 nCoV-19 vaccination. We report 474 SARS-CoV-2 infection episodes, including 48 individuals with hospitalization or death from COVID-19. Decreased magnitude of both the serological and the T cell response was associated with severe COVID-19. Overall, we identified clinical phenotypes that may benefit from targeted COVID-19 therapeutic strategies

Localisation of viruses in tuber tissues of potato

Poste

The relationship between terminology preferences, empowerment and internalised stigma in mental health

People with a mental illness may be exposed to stigma which, when internalised, negatively influences self-esteem, personal goal attainment and quality of life. However, people who are empowered may actively challenge stigma by engaging in meaningful opportunities, furthering their self-worth as they achieve control within their lives. People who are empowered through active treatment participation are more likely to reach recovery goals and experience the benefits of evidence-based practices. Hence, empowerment can be viewed as essential to the recovery and well-being of people with a mental illness. Participants in this quantitative study (N = 173) were diagnosed with a mental illness and were recruited via convenience sampling from mental health support groups in Australia. Internalised stigma was less common and empowerment more prevalent in this study than was found in previous studies. However high internalised stigma and low empowerment were present. The terms ‘individual’ and ‘person with lived experience’ which have been identified as the preferred terms in a previous study were highlighted as both empowering and recovery focussed

Development and validation of a high throughput, one-step, quantitative real-time RT-PCR assay for the simultaneous detection of PLRV, PVX, PVS and TSWV with a rapid RNA extraction method directly from bulked potato tuber samples

Potato is important in Western Australia both for domestic food production and export. Four viruses diminish tuber yield locally, Potato leaf roll virus (PLRV), Potato virus X (PVX), Potato virus S (PVS) and Tomato spotted wilt virus (TSWV). A real-time multiplex, single tube RT-PCR assay for the detection of these viruses from potato leaves and tubers was developed using Cy5-, FAM-, JOE- and ROX-labelled TaqMan probes. The copy numbers for transcripts were quantified with a dynamic range of 8x101 to 8x109 copies of PVX and PVS, 1x102 to 1x1010 copies of PLRV and 1x103 to 1x1010 copies of TSWV. The inter-assay reproducibility was high, with a coefficient of variation (CV) of <2%. Total RNA was rapidly and efficiently extracted from bulked tuber samples for the reliable detection of one or more of the viruses. These data indicate that this high-throughput test is accurate and sensitive, and will provide a cost-effective diagnostic tool for the seed potato industry

Quantitative analysis of anxiety levels of nursing students studying bioscience in Australia

Nursing students traditionally find bioscience difficult and anxiety provoking. This has important ramifications, as anxiety can hinder comprehension and lead to poor exam performance. The aim of the present study was to assess whether there was any difference between the anxiety levels of nursing students during their bioscience laboratory classes compared to their clinical laboratory classes. Students were recruited from a first year Bachelor of Nursing program. The self‐report State–Trait Anxiety Inventory (short form) was administered at the start of all classes throughout the semester. Anxiety scores of students between the units were compared using paired t‐tests, and repeated‐measures analysis of variance was used to measure anxiety scores within units over time. There were no significant differences in anxiety scores in the bioscience and clinical classes; however, the students were significantly more anxious in the theory classes. These findings suggest that nursing students do not find the subject of bioscience any more anxiety provoking than other nursing subjects. Bioscience educators should continue to focus on the integration of bioscience with nursing practice, while broader anxiety‐reduction strategies throughout the curriculum should be implemented

Is anxiety an issue for first year nursing students enrolled in bioscience units?

Knowledge of the biological sciences is an integral part of nursing care and hence an important component of the Bachelor of Nursing (BN) course. Although bioscience content has important relevance to nursing, students traditionally find bioscience difficult (Jordan et al 1999; Wharrad et al 1994) and anxiety-provoking (Nicoll and Butler 1996)

A single tube, quantitative real-time RT-PCR assay that detects four potato viruses simultaneously

A high throughput, real-time multiplex, single tube RT-PCR assay was developed for simultaneous detection of Potato leafroll virus (PLRV), Potato virus X (PVX) and Potato virus S (PVS) in potato leaves and tubers, and Tomato spotted wilt virus (TSWV) in potato tubers and tomato leaves. The test uses four different fluorescently labelled TaqMan® probes. Limits of detection sensitivity were established using a range of virus transcript copy numbers (8 × 101 to 8 × 109 copies of PVX and PVS, 1 × 102 to 1 × 1010 copies of PLRV and 1 × 103 to 1 × 1010 copies of TSWV). For each individual assay, the inter-assay reproducibility was high, with a coefficient of variation of the combined assays of <2%. Total RNA was extracted rapidly and efficiently from bulked samples equivalent to 300 dormant tubers to detect single infections of PLRV, PVX, PVS and TSWV simultaneously in a single assay. The multiplexed assay was validated in blind studies with leaves and tubers. This high-throughput test is accurate and sensitive, and provides seed potato industries with a cost-effective diagnostic tool to detect viruses reliably in bulked samples of dormant potato tubers

Recovery and borderline personality disorder: A description of the innovative open borders program

Although Recovery-oriented approaches to delivering mental health services are now promoted in health services across the globe, there is an ongoing need to adapt these approaches to meet the unique needs of consumers with a diagnosis of borderline personality disorder. The lived experience of borderline personality disorder includes emotional dysregulation, intense and unstable relationships, self-harming behaviours, fear of abandonment, and a limited capacity to cope with stress. These experiences present a range of challenges for those who deliver Recovery-oriented services and advocate the principles of empowerment and self-determination. This paper describes a novel crisis intervention program, “Open Borders,” which has been established to meet the unique needs of people with a borderline personality disorder diagnosis. Open Borders is a Recovery-oriented model that is run at a public, state-wide residential facility for mental health consumers in Western Australia, and offers alternative pathways to achieving mental health Recovery, including self-referral and short-term admission to a residential facility. The aims of the program are to break the cycle of hospital admission, reduce rates of self-harm, and support the complex Recovery journey of consumers with a diagnosis of borderline personality disorder. Open Borders provides an exemplar for other health service organisations seeking to establish Recovery-oriented crisis intervention alternatives

Open Borders

Borderline personality disorder is characterised by emotional instability, intense and unstable relationships and fear of abandonment..