Adenovirus-mediated gene delivery restores fertility in congenitally infertile female mice

Oogenesis depends on close interactions between oocytes and granulosa cells. Abnormal signaling between these cell types can result in infertility. However, attempts to manipulate oocyte-granulosa cell interactions have had limited success, likely due to the blood-follicle barrier (BFB), which prevents the penetration of exogenous materials into ovarian follicles. Here, we used adenoviruses (AVs) to manipulate the oocyte-granulosa cell interactions. AVs penetrated the BFB and transduced granulosa cells through ovarian microinjection. Although AVs caused transient inflammation, they did not impair fertility in wild-type mice. Introduction of Kitl-expressing AVs into congenitally infertile KitlSl-t/KitlSl-t mutant mouse ovaries, which contained only primordial follicles because of a lack of Kitl expression, restored fertility through natural mating. The offspring showed no evidence of AV integration and exhibited normal genomic imprinting patterns for imprinted genes. These results demonstrate the usefulness of AVs for manipulating oogenesis and suggest the possibility of gene therapies for human female infertility

Effects of nicotianamine on iron in the small intestine

Iron is an essential metal for all living organisms that is absorbed in the intestinal cells as a heme-chelated or free form. It is unclear how important plant-derived chelators, such as nicotianamine (NA), an organic small molecule that is ubiquitous in crops, vegetables, and various other foods, contribute to iron bioavailability in mammals. We performed electrophysiological assays with Xenopus laevis oocytes and radioactive tracer experiments with Caco-2 cells. The findings revealed that the proton-coupled amino acid transporter SLC36A1 (PAT1) transports iron in the form of NA-Fe (II) complex in vitro. Decreased expression of hPAT1 by RNA interference in Caco-2 cells reduced the uptake of NA-59Fe (II) complex. The uptake of inorganic 59Fe (II) was relatively unaffected. These results imply that PAT1 transports iron as a NA-Fe (II) complex. The rate of 59Fe absorption in the spleen, liver, and kidney was higher when mice were orally administered NA-59Fe (II) compared with free 59Fe (II). The profile of site-specific PAT1 expression in the mouse intestine coincided with those of NA and iron contents, which were the highest in the proximal jejunum. Orally administered NA-59Fe (II) complex in mice was detected in the proximal jejunum by thin layer chromatography. In contrast, much less 59Fe (or NA) was detected in the duodenum, where the divalent metal transporter SLC11A2 (DMT1) absorbs free Fe (II). The collective results revealed the role of PAT1 in NA-Fe (II) absorption in the intestine and potential implication of NA in iron uptake in mammals

Relation between urban volume and land surface temperature: A comparative study of planned and traditional cities in Japan

The horizontal two-dimensional (2D) urban land use approach is not sufficient to trace rapid changes in urban environment. Hence, a three-dimensional (3D) approach that is different from the traditional geographical method is necessary to understand the mechanism of compound urban diversity. Using remote sensing data captured in 2010/2011 and geospatial tools and techniques, we quantified the urban volume (UV, consisting of urban built volume (UBV) and urban green volume (UGV)) and retrieved and mapped the land surface temperature (LST) of two cities in Japan (Tsukuba, a planned city, and Tsuchiura, a traditional city). We compared these two cities in terms of (1) UBV and UGV and their relationships with mean LST; and (2) the relationship of the UGV–UBV ratio with mean LST. Tsukuba had a total UBV of 74 million m3, while Tsuchiura had a total of 89 million m3. In terms of UGV, Tsukuba had a total of 52 million m3, while Tsuchiura had a total of 29 million m3. In both cities, UBV had a positive relationship with mean LST (Tsukuba: R2 = 0.31, p < 0.001; Tsuchiura: R2 = 0.42, p < 0.001), and UGV had a negative relationship with mean LST (Tsukuba: R2 = 0.53, p < 0.001; Tsuchiura: R2 = 0.19, p < 0.001). Tsukuba also had a higher UGV–UBV ratio of 54.9% in comparison with Tsuchiura, with 28.7%. Overall, the results indicate that mean LST was more intense in the traditional city (Tsuchiura). This could have been due to the difference in urban spatial structure. As a planned city, Tsukuba is still a relatively young city that has more dispersed green spaces and a well-spread (so far) built-up area

An interplay of NOX1-derived ROS and oxygen determines the spermatogonial stem cell self-renewal efficiency under hypoxia

酸素による活性酸素制御が精子形成に果たす役割を解明 --活性酸素制御の破綻が男性不妊を引き起こす--. 京都大学プレスリリース. 2021-01-15.Reactive oxygen species (ROS) produced by NADPH1 oxidase 1 (NOX1) are thought to drive spermatogonial stem cell (SSC) self-renewal through feed-forward production of ROS by the ROS-BCL6B-NOX1 pathway. Here we report the critical role of oxygen on ROS-induced self-renewal. Cultured SSCs proliferated poorly and lacked BCL6B expression under hypoxia despite increase in mitochondria-derived ROS. Due to lack of ROS amplification under hypoxia, NOX1-derived ROS were significantly reduced, and Nox1-deficient SSCs proliferated poorly under hypoxia but normally under normoxia. NOX1-derived ROS also influenced hypoxic response in vivo because Nox1-deficient undifferentiated spermatogonia showed significantly reduced expression of HIF1A, a master transcription factor for hypoxic response. Hypoxia-induced poor proliferation occurred despite activation of MYC and suppression of CDKN1A by HIF1A, whose deficiency exacerbated self-renewal efficiency. Impaired proliferation of Nox1- or Hif1a-deficient SSCs under hypoxia was rescued by Cdkn1a depletion. Consistent with these observations, Cdkn1a-deficient SSCs proliferated actively only under hypoxia but not under normoxia. On the other hand, chemical suppression of mitochondria-derived ROS or Top1mt mitochondria-specific topoisomerase deficiency did not influence SSC fate, suggesting that NOX1-derived ROS play a more important role in SSCs than mitochondria-derived ROS. These results underscore the importance of ROS origin and oxygen tension on SSC self-renewal

Impact of Landscape Structure on the Variation of Land Surface Temperature in Sub-Saharan Region: A Case Study of Addis Ababa using Landsat Data (1986–2016)

Urbanization has bloomed across Asia and Africa of late, while two centuries ago, it was confined to developed regions in the largest urban agglomerations. The changing urban landscape can cause irretrievable changes to the biophysical environment, including changes in the spatiotemporal pattern of the land surface temperature (LST). Understanding these variations in the LST will help us introduce appropriate mitigation techniques to overcome negative impacts. The research objective was to assess the impact of landscape structure on the variation in LST in the African region as a geospatial approach in Addis Ababa, Ethiopia from 1986–2016 with fifteen-year intervals. Land use and land cover (LULC) mapping and LST were derived by using pre-processed Landsat data (Level 2). Gradient analysis was computed for the pattern of the LST from the city center to the rural area, while intensity calculation was facilitated to analyze the magnitude of LST. Directional variation of the LST was not covered by the gradient analysis. Hence, multidirectional and multitemporal LST profiles were employed over the orthogonal and diagonal directions. The result illustrated that Addis Ababa had undergone rapid expansion. In 2016, the impervious surface (IS) had dominated 33.8% of the total lands. The IS fraction ratio of the first zone (URZ1) has improved to 66.2%, 83.7%, and 87.5%, and the mean LST of URZ1 has improved to 25.2 °C, 26.6 °C, and 29.6 °C in 1986, 2001, and 2016, respectively. The IS fraction has gradually been declining from the city center to the rural area. The behavior of the LST is not continually aligning with a pattern of IS similar to other cities along the URZs. After the specific URZs (zone 17, 37, and 41 in 1986, 2001, and 2016, respectively), the mean LST shows an increasing trend because of a fraction of bare land. This trend is different from those of other cities even in the tropical regions. The findings of this study are useful for decision makers to introduce sustainable landscape and urban planning to create livable urban environments in Addis Ababa, Ethiopia

Impact of Urban Surface Characteristics and Socio-Economic Variables on the Spatial Variation of Land Surface Temperature in Lagos City, Nigeria

The urban heat island (UHI) and its consequences have become a key research focus of various disciplines because of its negative externalities on urban ecology and the total livability of cities. Identifying spatial variation of the land surface temperature (LST) provides a clear picture to understand the UHI phenomenon, and it will help to introduce appropriate mitigation technique to address the advanced impact of UHI. Hence, the aim of the research is to examine the spatial variation of LST concerning the UHI phenomenon in rapidly urbanizing Lagos City. Four variables were examined to identify the impact of urban surface characteristics and socio-economic activities on LST. The gradient analysis was employed to assess the distribution outline of LST from the city center point to rural areas over the vegetation and built-up areas. Partial least square (PLS) regression analysis was used to assess the correlation and statistically significance of the variables. Landsat data captured in 2002 and 2013 were used as primary data sources and other gridded data, such as PD and FFCOE, were employed. The results of the analyses show that the distribution pattern of the LST in 2002 and 2013 has changed over the study period as results of changing urban surface characteristics (USC) and the influence of socio-economic activities. LST has a strong positive relationship with NDBI and a strong negative relationship with NDVI. The rapid development of Lagos City has been directly affected by conversion more green areas to build up areas over the time, and it has resulted in formulating more surface urban heat island (SUHI). Further, the increasing population and their socio-economic activities including industrialization and infrastructure development have also caused a significant impact on LST changes. We recommend that the results of this research be used as a proxy tool to introduce appropriate landscape and town planning in a sustainable viewpoint to make healthier and livable urban environments in Lagos City, Nigeria

Quantifying Surface Urban Heat Island Formation in the World Heritage Tropical Mountain City of Sri Lanka

Presently, the urban heat island (UHI) phenomenon, and its adverse impacts, are becoming major research foci in various interrelated fields due to rapid changes in urban ecological environments. Various cities have been investigated in previous studies, and most of the findings have facilitated the introduction of proper mitigation measures to overcome the negative impact of UHI. At present, most of the mountain cities of the world have undergone rapid urban development, and this has resulted in the increasing surface UHI (SUHI) phenomenon. Hence, this study focuses on quantifying SUHI in Kandy City, the world heritage tropical mountain city of Sri Lanka, using Landsat data (1996 and 2017) based on the mean land surface temperature (LST), the difference between the fraction of impervious surfaces (IS), and the fraction of green space (GS). Additionally, we examined the relationship of LST to the green space/impervious surface fraction ratio (GS/IS fraction ratio) and the magnitude of the GS/IS fraction ratio. The SUHI intensity (SUHII) was calculated based on the temperature difference between main land use/cover categories and the temperature difference between urban-rural zones. We demarcated the rural zone based on the fraction of IS recorded, <10%, along with the urban-rural gradient zone. The result shows a SUHII increase from 3.9 °C in 1996 to 6.2 °C in 2017 along the urban-rural gradient between the urban and rural zones (10 < IS). These results relate to the rapid urban expansion of the study areas from 1996 to 2017. Most of the natural surfaces have changed to impervious surfaces, causing an increase of SUHI in Kandy City. The mean LST has a positive relationship with the fraction of IS and a negative relationship with the fraction of GS. Additionally, the GS/IS fraction ratio shows a rapid decline. Thus, the findings of this study can be considered as a proxy indicator for introducing proper landscape and urban planning for the World Heritage tropical mountain city of Kandy in Sri Lanka