The “resurrection method” for modification of specific proteins in higher plants

AbstractWe describe a new method designated “the resurrection method” by which a modified protein is expressed in higher plants in place of the original protein. The modified gene constructed by introducing synonymous codon substitutions throughout the original gene to prevent the sequence-specific degradation of its mRNA during RNA silencing is expressed while the expression of the original gene is suppressed. Here, we report the successful alteration of the biochemical properties of green fluorescent protein expressed in transgenic Nicotiana benthamiana, suggesting that this method could be useful for gene control in living plants

Universal dark halo scaling relation for the dwarf spheroidal satellites

Motivated by a recently found interesting property of the dark halo surface density within a radius, $r_{\rm max}$, giving the maximum circular velocity, $V_{\rm max}$, we investigate it for dark halos of the Milky Way's and Andromeda's dwarf satellites based on cosmological simulations. We select and analyze the simulated subhalos associated with Milky Way-sized dark halos and find that the values of their surface densities, $\Sigma_{V_{\rm max}}$, are in good agreement with those for the observed dwarf spheroidal satellites even without employing any fitting procedures. This implies that this surface density would not be largely affected by any baryonic feedbacks and thus universal. Moreover, all subhalos on the small scales of dwarf satellites are expected to obey the relation $\Sigma_{V_{\rm max}}\propto V_{\rm max}$, irrespective of differences in their orbital evolutions, host halo properties, and observed redshifts. Therefore, we find that the universal scaling relation for dark halos on dwarf galaxy mass scales surely exists and provides us important clues to understanding fundamental properties of dark halos. We also investigate orbital and dynamical evolutions of subhalos to understand the origin of this universal dark halo relation and find that most of subhalos evolve generally along the $r_{\rm max}\propto V_{\rm max}$ sequence, even though these subhalos have undergone different histories of mass assembly and tidal stripping. This sequence, therefore, should be the key feature to understand the nature of the universality of $\Sigma_{V_{\rm max}}$.Comment: 12 pages, 5 figures and 3 tables, submitted to Ap

Hopf Algebra Symmetry and String Theory

We investigate the Hopf algebra structure in string worldsheet theory and give a unified formulation of the quantization of string and the space-time symmetry. We reformulate the path integral quantization of string as a Drinfeld twist at the worldsheet level. The coboundary relation shows that the Drinfeld twist defines a module algebra which is equivalent to operators with normal ordering. Upon applying the twist, the space-time diffeomorphism is deformed into a twisted Hopf algebra, while the Poincar\'e symmetry is unchanged. This suggests a characterization of the symmetry: unbroken symmetries are twist invariant Hopf subalgebras, while broken symmetries are realized as twisted ones. We provide arguments that relate this twisted Hopf algebra to symmetries in path integral quantization.Comment: 35 pages, no figure, v2: references and comments added, typos corrected, v3: requires PTP style, title changed, final version published in PT

Effective treatment of a 13-year-old boy with steroid-dependent ocular myasthenia gravis using tacrolimus

Over the past several years, tacrolimus has attracted attention as a new therapeutic drug for myasthenia gravis (MG), but few reports have considered its use for MG in pediatric patients, and most of these have focused on severe systemic MG. In this case report, we used tacrolimus to successfully treat a 13-year-old boy with ocular MG who had suffered from severe steroid complications, including a failure of thrive and osteoporosis. He first showed symptoms of ocular MG at age 2 years 3 months. At age 13 years, he was receiving PSL (3.75 mg/day), but the symptoms of ocular MG recurred. We increased the dosage of oral PSL up to 30 mg/day, and three courses of mPSL pulse therapy were applied, but these therapies had only limited effect, and his symptoms worsened. Tacrolimus was started at 0.4mg/day (0.011mg/kg/day), and every two weeks the dose was gradually increased by 0.2mg/day. His symptoms of MG began to improve three weeks after the initial administration of tacrolimus. Approximately three months after the start of tacrolimus administration, PSL was discontinued. Currently, at one year and four months after the start of tacrolimus administration, while slight ptosis is observed in the evening, it does not influence his daily life, and his condition remains comparable to that when he stopped taking PSL. No adverse effects of tacrolimus have been recognized. In pediatric patients with steroid-dependent ocular MG without thymectomy, tacrolimus may be a safe and effective alternative to steroid and thymectomy

Fission yeast MOZART1/Mzt1 is an essential γ-tubulin complex component required for complex recruitment to the microtubule organizing center, but not its assembly

γ-Tubulin plays a universal role in microtubule nucleation from microtubule organizing centers (MTOCs) such as the animal centrosome and fungal spindle pole body (SPB). γ-Tubulin functions as a multiprotein complex called the γ-tubulin complex (γ-TuC), consisting of GCP1–6 (GCP1 is γ-tubulin). In fungi and flies, it has been shown that GCP1–3 are core components, as they are indispensable for γ-TuC complex assembly and cell division, whereas the other three GCPs are not. Recently a novel conserved component, MOZART1, was identified in humans and plants, but its precise functions remain to be determined. In this paper, we characterize the fission yeast homologue Mzt1, showing that it is essential for cell viability. Mzt1 is present in approximately equal stoichiometry with Alp4/GCP2 and localizes to all the MTOCs, including the SPB and interphase and equatorial MTOCs. Temperature-sensitive mzt1 mutants display varying degrees of compromised microtubule organization, exhibiting multiple defects during both interphase and mitosis. Mzt1 is required for γ-TuC recruitment, but not sufficient to localize to the SPB, which depends on γ-TuC integrity. Intriguingly, the core γ-TuC assembles in the absence of Mzt1. Mzt1 therefore plays a unique role within the γ-TuC components in attachment of this complex to the major MTOC site.This research was supported by Cancer Research UK (T.T.)

Possible Charge-Exchange X-Ray Emission in the Cygnus Loop Detected with Suzaku

X-ray spectroscopic measurements of the Cygnus Loop supernova remnant indicate that metal abundances throughout most of the remnant's rim are depleted to about 0.2 times the solar value. However, recent X-ray studies have revealed in some narrow regions along the outermost rim anomalously "enhanced" abundances (up to about 1 solar). The reason for these anomalous abundances is not understood. Here, we examine X-ray spectra in annular sectors covering nearly the entire rim of the Cygnus Loop using Suzaku (21 pointings) and XMM-Newton (1 pointing). We find that spectra in the "enhanced" abundance regions commonly show a strong emission feature at about 0.7 keV. This feature is likely a complex of He-like O K(gamma + delta + epsilon), although other possibilities cannot be fully excluded. The intensity of this emission relative to He-like O Kalpha appears to be too high to be explained as thermal emission. This fact, as well as the spatial concentration of the anomalous abundances in the outermost rim, leads us to propose an origin from charge-exchange processes between neutrals and H-like O. We show that the presence of charge-exchange emission could lead to the inference of apparently "enhanced" metal abundances using pure thermal emission models. Accounting for charge-exchange emission, the actual abundances could be uniformly low throughout the rim. The overall abundance depletion remains an open question.Comment: Published in Ap

Chandra Observations of the Northeastern Rim of the Cygnus Loop

We present results from spatially resolved spectral analyses of the northeastern (NE) rim of the Cygnus Loop supernova remnant (SNR) based on two Chandra observations. One pointing includes northern outermost abundance-enhanced regions discovered by recent Suzaku observations, while the other pointing is located on regions with "normal" abundances in the NE rim of the Cygnus Loop. The superior spatial resolving power of Chandra allows us to reveal that the abundance-enhanced region is concentrated in an about 200"-thickness region behind the shock front. We confirm absolute metal abundances (i.e., relative to H) as well as abundance ratios between metals are consistent with those of the solar values within a factor of about 2. Also, we find that the emission measure in the region gradually decreases toward the shock front. These features are in contrast with those of the ejecta fragments around the Vela SNR, which leads us to believe that the abundance enhancements are not likely due to metal-rich ejecta. We suggest that the origin of the plasma in this region is the interstellar medium (ISM). In the "normal" abundance regions, we confirm that abundances are depleted to the solar values by a factor of about 5 that is not expected in the ISM around the Cygnus Loop. Introduction of non-thermal emission in our model fitting can not naturally resolve the abundance-depletion problem. The origin of the depletion still remains as an open question.Comment: 18 pages, 6 figure

PPAR-α transcriptional activity is required to combat doxorubicin-induced podocyte injury in mice.

Immunosuppressants and inhibitors of the renin angiotensin system are major reagents to treat nephrotic syndrome but their clinical effects are not necessarily satisfactory. Injection of doxorubicin in several strains of mice causes nephrotic syndrome-like disorder. Zhou et al. report that PPAR-α expression is downregulated in murine doxorubicin nephropathy and a PPAR-α agonist, fenofibrate, partially ameliorates the disorder induced likely through stabilization of nephrin expression and suppression of apoptosis in podocytes, providing a new preventive strategy